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Species: Caenorhabditis elegans
Genetic Insert: pmex-5::mCherryo::PH::tbb-2
Vector Backbone Description: Vector Backbone:modified pCFJ150; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27385332
Proper citation: RRID:Addgene_73511 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmex-5::TagRFP-T::PH::tbb-2
Vector Backbone Description: Vector Backbone:modified pCFJ150; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27385332
Proper citation: RRID:Addgene_73517 Copy
Species: Caenorhabditis elegans
Genetic Insert: Punc-17::2xNLS-FLP-D5::let-858 3'-UTR
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:Worm Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26837755
Proper citation: RRID:Addgene_73685 Copy
Species: Caenorhabditis elegans
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Erik Jorgensen; Backbone Size:8941; Vector Backbone:pCFJ355; Vector Types:Worm Expression, Cre/Lox, MosSCI; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26144318
Proper citation: RRID:Addgene_69156 Copy
Species: Caenorhabditis elegans
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Erik Jorgensen; Backbone Size:7517; Vector Backbone:pCFJ350; Vector Types:Worm Expression, Cre/Lox, MosSCI; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26144318
Comments: discrepancies between QC and full sequence should not have functional consequences
Proper citation: RRID:Addgene_69155 Copy
Species: Caenorhabditis elegans
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Erik Jorgensen; Backbone Size:7606; Vector Backbone:pCFJ356; Vector Types:Worm Expression, Cre/Lox, MosSCI; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26144318
Comments: discrepancies between QC and full sequence should not have functional consequences
Proper citation: RRID:Addgene_69154 Copy
Species: Caenorhabditis elegans
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Erik Jorgensen; Backbone Size:7517; Vector Backbone:pCFJ350; Vector Types:Worm Expression, Cre/Lox, MosSCI; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26144318
Proper citation: RRID:Addgene_69152 Copy
Species: Caenorhabditis elegans
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Erik Jorgensen; Backbone Size:7606; Vector Backbone:pCFJ356; Vector Types:Worm Expression, Cre/Lox, MosSCI; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26144318
Comments: Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_69151 Copy
Species: Caenorhabditis elegans
Genetic Insert: Cre recombinase
Vector Backbone Description: Backbone Marker:Erik Jorgensen; Backbone Size:8941; Vector Backbone:pCFJ355; Vector Types:Worm Expression, Cre/Lox, MosSCI; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26144318
Proper citation: RRID:Addgene_69255 Copy
Species: Caenorhabditis elegans
Genetic Insert: Cre recombinase
Vector Backbone Description: Backbone Marker:Erik Jorgensen; Backbone Size:8941; Vector Backbone:pCFJ355; Vector Types:Worm Expression, Cre/Lox, MosSCI; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26144318
Proper citation: RRID:Addgene_69254 Copy
Species: Caenorhabditis elegans
Genetic Insert: Cre recombinase
Vector Backbone Description: Backbone Marker:Erik Jorgensen; Backbone Size:8941; Vector Backbone:pCFJ355; Vector Types:Worm Expression, Cre/Lox, MosSCI; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26144318
Proper citation: RRID:Addgene_69253 Copy
Species: Caenorhabditis elegans
Genetic Insert: Cre recombinase
Vector Backbone Description: Backbone Marker:Erik Jorgensen; Backbone Size:8941; Vector Backbone:pCFJ355; Vector Types:Worm Expression, Cre/Lox, MosSCI; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26144318
Proper citation: RRID:Addgene_69258 Copy
Species: Caenorhabditis elegans
Genetic Insert: Cre recombinase
Vector Backbone Description: Backbone Marker:Erik Jorgensen; Backbone Size:8941; Vector Backbone:pCFJ355; Vector Types:Worm Expression, Cre/Lox, MosSCI; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26144318
Proper citation: RRID:Addgene_69256 Copy
Species: Caenorhabditis elegans
Genetic Insert: sgRNA(F+E) targeting pha-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3519; Vector Backbone:pCR-Blunt II-TOPO; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_69488 Copy
Species: Caenorhabditis elegans
Genetic Insert: mCherry::linker::H2B
Vector Backbone Description: Backbone Marker:Messing lab Plasmid 50005; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26187122
Proper citation: RRID:Addgene_70049 Copy
Species: Caenorhabditis elegans
Genetic Insert: TagRFP-T-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Comments: pDD286 is a derivative of our published TagRFP-SEC vector pDD284 (Dickinson et al. Genetics 2015, Addgene plasmid number 66825). It has a 3xMyc tag in place of 3xFlag, and Lox2272 sites in place of LoxP. These features allow pDD286 to be used in a genetic background that has already been modified using a green FP-SEC vector, without conflicts between epitope tags and Lox sites.
Proper citation: RRID:Addgene_70684 Copy
Species: Caenorhabditis elegans
Genetic Insert: mKate2-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26044593
Comments: pDD287is a derivative of our published TagRFP-SEC vector pDD285 (Dickinson et al. Genetics 2015, Addgene plasmid number 66825). It has a 3xMyc tag in place of 3xFlag, and Lox2272 sites in place of LoxP. These features allow pDD287 to be used in a genetic background that has already been modified using a green FP-SEC vector, without conflicts between epitope tags and Lox sites.
Proper citation: RRID:Addgene_70685 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16
Vector Backbone Description: Backbone Marker:Blelloch et al., 1999; Mathies et al., 2003; Vector Backbone:pJK590; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20110332
Proper citation: RRID:Addgene_71363 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16
Vector Backbone Description: Backbone Marker:Libina, et al. 2003. PMID 14622602; Vector Backbone:pNL205; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25960195
Proper citation: RRID:Addgene_71364 Copy
Species: Caenorhabditis elegans
Genetic Insert: sgRNA for APa13
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25249454
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_65591 Copy
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