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Vector Backbone Description: Vector Backbone:pTcTurboID-GW; Vector Types:Trypanosomatid expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: If you want to perform traditional cloning (instead of Gateway recombiantion), it can be achieved by replacing the GFP cassette from pTcTurboID-GW_GFP with the desired CDS using BamHI and EcoRV restriction enzymes. Also, any CDS can be cloned in frame to GFP using only EcoRV. 5' Cloning Site: ClaI (not destroyed), 3' Cloning Site: ClaI (not destroyed).
Proper citation: RRID:Addgene_234714 Copy
Vector Backbone Description: Vector Backbone:Custom (R6K origin); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40048245
Proper citation: RRID:Addgene_238493 Copy
Vector Backbone Description: Vector Backbone:Custom (R6K origin); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40048245
Proper citation: RRID:Addgene_238494 Copy
Genetic Insert: epegRNA
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40555761
Comments: Please visit https://doi.org/10.1101/2024.06.16.599253 for the bioRxiv preprint.
Proper citation: RRID:Addgene_240539 Copy
Species: Synthetic
Genetic Insert: attR1-CmR-ccdB-attR2
Vector Backbone Description: Vector Backbone:pCAGEN-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_242575 Copy
Vector Backbone Description: Vector Backbone:pCAGEN-DEST; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_242573 Copy
Vector Backbone Description: Vector Backbone:pCAGEN-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_242577 Copy
Vector Backbone Description: Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:11967; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The insert begins with a Gateway attR destination cloning cassette (for insertion of gene regulatory sequences), followed by a DSCP synthetic core promoter, a Syn21 translation enhancer, a QF2 transcription factor followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint.
Proper citation: RRID:Addgene_173728 Copy
Vector Backbone Description: Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:11484; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The insert begins with 20 copies of the UAS binding sequence followed by a DSCP synthetic core promoter, a Gateway attR destination cloning cassette followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint.
Proper citation: RRID:Addgene_173723 Copy
Vector Backbone Description: Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:11397; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The insert begins with 13 copies of the LexA binding sequence followed by a DSCP synthetic core promoter, a Gateway attR destination cloning cassette followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint.
Proper citation: RRID:Addgene_173724 Copy
Vector Backbone Description: Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:11189; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The insert begins with 10 copies of the UAS binding sequence followed by a DSCP synthetic core promoter, a Gateway attR destination cloning cassette followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint.
Proper citation: RRID:Addgene_173725 Copy
Vector Backbone Description: Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:12063; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The insert begins with a Gateway attR destination cloning cassette (for insertion of gene regulatory sequences), followed by a DSCP synthetic core promoter, a Syn21 translation enhancer, a LexA:QF hybrid transcription factor (LexA DNA binding domain, QF activation domain) followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint.
Proper citation: RRID:Addgene_173727 Copy
Vector Backbone Description: Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:13668; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The insert begins with a Gateway attR destination cloning cassette (for insertion of gene regulatory sequences), followed by a DSCP synthetic core promoter, a Syn21 translation enhancer, a QF:GAL4 hybrid transcription factor (QF DNA binding domain, Gal4 activation domain) followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint.
Proper citation: RRID:Addgene_173729 Copy
Vector Backbone Description: Vector Backbone:pUC57-pB-2xIns-Flk-iCre-WPRE-pA; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229818 Copy
Genetic Insert: epegRNA
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40555761
Comments: Please visit https://doi.org/10.1101/2024.06.16.599253 for the bioRxiv preprint.
Proper citation: RRID:Addgene_240538 Copy
Vector Backbone Description: Backbone Size:5110; Vector Backbone:pSTARR-seq_human; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:32958950
Proper citation: RRID:Addgene_157874 Copy
Genetic Insert: mir-1-1 reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression, RNAi, Expression of query pri-miRNA sequences in HEK293 cells; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23415231
Comments: Query pri-miRNA sequences are to be recombined into this plasmid using Gateway recombination. The human pri-mir-1-1 sequence is cloned downstream of the query pri-miRNA and is transcriptionally fused to the query pri-miRNA sequence. This plasmid was used for ectopic pri-miRNA expression in HEK293 cells
Proper citation: RRID:Addgene_46646 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4349; Vector Backbone:pMT/BiP/V5/His A; Vector Types:Insect Expression, Secreted expression in Drosophila Culture; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23827685
Comments: Inducible with 0.8 uM Copper Sulfate. Targets proteins to the extracellular millieu using the Drosophila melanogaster BiP signal peptide. Expresses in Drosophila melanogaster cell lines, such as Schneider 2 (S2) cells. Published in Ozkan et al, Cell 154(1): 228 (2013); PMID: 23827685; doi: 10.1016/j.cell.2013.06.006
After recombination, transform and grow in DH5alpha. The plasmid is no longer chloramphenicol resistant after recombination.
Proper citation: RRID:Addgene_47032 Copy
Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:5779; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47074 Copy
Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:5779; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47072 Copy
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