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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pTcTurboID-GW
 
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RRID:Addgene_234714 Chloramphenicol and Ampicillin If you want to perform traditional cloning (instead of Gateway recombiantion), it can be achieved by replacing the GFP cassette from pTcTurboID-GW_GFP with the desired CDS using BamHI and EcoRV restriction enzymes. Also, any CDS can be cloned in frame to GFP using only EcoRV. 5' Cloning Site: ClaI (not destroyed), 3' Cloning Site: ClaI (not destroyed). Vector Backbone:pTcTurboID-GW; Vector Types:Trypanosomatid expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:32:58 0
pLP-PH16_RS08125-pcepA-himar-chlorR
 
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RRID:Addgene_238493 Chloramphenicol and Ampicillin PMID:40048245 Vector Backbone:Custom (R6K origin); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:33:01 0
pLP-PH16_RS09790-pcepA-himar-chlorR
 
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RRID:Addgene_238494 Chloramphenicol and Ampicillin PMID:40048245 Vector Backbone:Custom (R6K origin); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:33:01 0
pLenti-U6-epegRNA-SV40-puro
 
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RRID:Addgene_240539 epegRNA Chloramphenicol and Ampicillin PMID:40555761 Please visit https://doi.org/10.1101/2024.06.16.599253 for the bioRxiv preprint. Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:33:57 0
pCAGEN-DV-IRES-myr-mKate (JDW 494)
 
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RRID:Addgene_242575 attR1-CmR-ccdB-attR2 Synthetic Chloramphenicol and Ampicillin PMID:41230856 Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint. Vector Backbone:pCAGEN-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:34:01 0
pCAGEN-DEST (JDW 471)
 
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RRID:Addgene_242573 Chloramphenicol and Ampicillin PMID:41230856 Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint. Vector Backbone:pCAGEN-DEST; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:34:04 0
pMef2c-AHF-DEST (JDW 476)
 
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RRID:Addgene_242577 Chloramphenicol and Ampicillin PMID:41230856 Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint. Vector Backbone:pCAGEN-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:34:01 0
pBID2_Gateway>QF2
 
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RRID:Addgene_173728 Chloramphenicol and Ampicillin The insert begins with a Gateway attR destination cloning cassette (for insertion of gene regulatory sequences), followed by a DSCP synthetic core promoter, a Syn21 translation enhancer, a QF2 transcription factor followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint. Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:11967; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin QF2 2026-08-15 01:34:03 0
pBID2_UAS>Gateway
 
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RRID:Addgene_173723 Chloramphenicol and Ampicillin The insert begins with 20 copies of the UAS binding sequence followed by a DSCP synthetic core promoter, a Gateway attR destination cloning cassette followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint. Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:11484; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin 20 x UAS sequences 2026-08-15 01:34:03 0
pBID2_LexOp>Gateway
 
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RRID:Addgene_173724 Chloramphenicol and Ampicillin The insert begins with 13 copies of the LexA binding sequence followed by a DSCP synthetic core promoter, a Gateway attR destination cloning cassette followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint. Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:11397; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin 13 x LexOp sequences 2026-08-15 01:34:05 0
pBID2_QUAS>Gateway
 
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RRID:Addgene_173725 Chloramphenicol and Ampicillin The insert begins with 10 copies of the UAS binding sequence followed by a DSCP synthetic core promoter, a Gateway attR destination cloning cassette followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint. Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:11189; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin 10 x QUAS sequences 2026-08-15 01:34:03 0
pBID2_Gateway>LexQF
 
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RRID:Addgene_173727 Chloramphenicol and Ampicillin The insert begins with a Gateway attR destination cloning cassette (for insertion of gene regulatory sequences), followed by a DSCP synthetic core promoter, a Syn21 translation enhancer, a LexA:QF hybrid transcription factor (LexA DNA binding domain, QF activation domain) followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint. Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:12063; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin LexA DNA binding domain, QF activation domain 2026-08-15 01:34:05 0
pBID2_Gateway>QFG4
 
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RRID:Addgene_173729 Chloramphenicol and Ampicillin The insert begins with a Gateway attR destination cloning cassette (for insertion of gene regulatory sequences), followed by a DSCP synthetic core promoter, a Syn21 translation enhancer, a QF:GAL4 hybrid transcription factor (QF DNA binding domain, Gal4 activation domain) followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint. Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:13668; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin QF DNA binding domain, GAL4 activation domain 2026-08-15 01:34:03 0
pB-2x-lns-DEST (JDW 1206)
 
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RRID:Addgene_229818 Chloramphenicol and Ampicillin PMID:41230856 Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint. Vector Backbone:pUC57-pB-2xIns-Flk-iCre-WPRE-pA; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:33:27 0
pLenti-U6-epegRNA-SV40-mCherry
 
Resource Report
Resource Website
RRID:Addgene_240538 epegRNA Chloramphenicol and Ampicillin PMID:40555761 Please visit https://doi.org/10.1101/2024.06.16.599253 for the bioRxiv preprint. Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:33:36 0
pDEST-hSTARR-luc-Pmyc
 
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RRID:Addgene_157874 Chloramphenicol and Ampicillin PMID:32958950 Backbone Size:5110; Vector Backbone:pSTARR-seq_human; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:33:36 0
pcDNA3.2 V5-DEST mir-1-1 reporter
 
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RRID:Addgene_46646 mir-1-1 reporter Chloramphenicol and Ampicillin PMID:23415231 Query pri-miRNA sequences are to be recombined into this plasmid using Gateway recombination. The human pri-mir-1-1 sequence is cloned downstream of the query pri-miRNA and is transcriptionally fused to the query pri-miRNA sequence. This plasmid was used for ectopic pri-miRNA expression in HEK293 cells Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression, RNAi, Expression of query pri-miRNA sequences in HEK293 cells; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:15:33 0
pECIA2
 
Resource Report
Resource Website
RRID:Addgene_47032 Chloramphenicol and Ampicillin PMID:23827685 Inducible with 0.8 uM Copper Sulfate. Targets proteins to the extracellular millieu using the Drosophila melanogaster BiP signal peptide. Expresses in Drosophila melanogaster cell lines, such as Schneider 2 (S2) cells. Published in Ozkan et al, Cell 154(1): 228 (2013); PMID: 23827685; doi: 10.1016/j.cell.2013.06.006 After recombination, transform and grow in DH5alpha. The plasmid is no longer chloramphenicol resistant after recombination. Backbone Marker:Invitrogen; Backbone Size:4349; Vector Backbone:pMT/BiP/V5/His A; Vector Types:Insect Expression, Secreted expression in Drosophila Culture; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:15:37 0
pBXNAC3H
 
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1+ mentions
RRID:Addgene_47074 Chloramphenicol and Ampicillin PMID:21410291 Backbone Marker:LM Guzman; Backbone Size:5779; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:15:37 1
pBXCA3H
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_47072 Chloramphenicol and Ampicillin PMID:21410291 Backbone Marker:LM Guzman; Backbone Size:5779; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:15:38 2

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