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Species: Homo sapiens
Genetic Insert: RAB14 3' UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7350; Vector Backbone:pmirGLO Dual-Luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25312678
Proper citation: RRID:Addgene_61489 Copy
Genetic Insert: aadA
Vector Backbone Description: Vector Backbone:pDIM-C8-BLA; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23284860
Proper citation: RRID:Addgene_61531 Copy
Species: Homo sapiens
Genetic Insert: HaloTag-GFP-Nuf2
Vector Backbone Description: Backbone Marker:Dr. Eugene Makeyev; Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25400104
Proper citation: RRID:Addgene_61499 Copy
Species: Mus musculus
Genetic Insert: Bmi1
Vector Backbone Description: Backbone Size:8376; Vector Backbone:TetO-FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25454632
Proper citation: RRID:Addgene_61532 Copy
Species: Homo sapiens
Genetic Insert: NOTCH-ICD
Vector Backbone Description: Backbone Size:8376; Vector Backbone:TetO-FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25454632
Proper citation: RRID:Addgene_61540 Copy
Species: Synthetic
Genetic Insert: HrpL-rbs34-CI-LVA-t-plam-30gfp-t
Vector Backbone Description: Backbone Marker:BioBrick registry; Backbone Size:3547; Vector Backbone:pSB4A3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22009040
Proper citation: RRID:Addgene_61439 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtU6-26:sgRNA
Vector Backbone Description: Backbone Marker:Promega Corp.; Backbone Size:3000; Vector Backbone:pGEM-T-easy; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24836556
Proper citation: RRID:Addgene_61432 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:7100; Vector Backbone:pPZP221; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:24836556
Proper citation: RRID:Addgene_61433 Copy
Species: Synthetic
Genetic Insert: chromatin insulator flanked TRE-LSL-tdTomato
Vector Backbone Description: Backbone Size:2682; Vector Backbone:pUC57; Vector Types:Recombinase-mediated cassette exchange using Flp recombinase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25741722
Proper citation: RRID:Addgene_61576 Copy
Species: Mus musculus
Genetic Insert: Slc17a7-IRES2-Cre
Vector Backbone Description: Backbone Size:2657; Vector Backbone:minimal cloning vector; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25741722
Comments: The Zeng lab recommends amplifying this plasmid in 15 microg/ml kanamycin to reduce recombination. Addgene is not able to supply the plasmid under these selection conditions and the stab you receive will be under Amp selection. We recommend using the low conc. Kan selection and checking your plasmid prep for recombination.
Proper citation: RRID:Addgene_61574 Copy
Species: Homo sapiens
Genetic Insert: BRCA1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: In frame stop codon between the HindIII site and ATG of BRCA1 cDNA.
Proper citation: RRID:Addgene_61586 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Lyn-AKAR4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20838685
Proper citation: RRID:Addgene_61620 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AKAR4-Kras
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20838685
Proper citation: RRID:Addgene_61621 Copy
Species: Synthetic
Genetic Insert: ntdTomato
Vector Backbone Description: Backbone Size:2580; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25643149
Comments: Please note that Addgene NGS results identified as stop codon after the second EGFP ORF. This stop codon was present in the original, published version of the plasmid, such that the insert is n2GFP rather than n3GFP. The plasmid functions as described in the associated publication. Co-expression with the helper plasmid pSOUP is suggested for expression in Agrobacterium.
The diagnostic digest indicates this plasmid may be bigger than the assembled sequence suggests.
Proper citation: RRID:Addgene_61628 Copy
Species: Homo sapiens
Genetic Insert: Cezanne2
Vector Backbone Description: Backbone Marker:OPPF-UK; Backbone Size:6299; Vector Backbone:pOPINK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23827681
Comments: This construct is the Isoform 2 of Cezanne2.
Proper citation: RRID:Addgene_61582 Copy
Vector Backbone Description: Backbone Size:2959; Vector Backbone:pBS SK2+; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25741722
Comments: The Zeng lab recommends amplifying this plasmid in 15 microg/ml kanamycin to reduce recombination. Addgene is not able to supply the plasmid under these selection conditions and the stab you receive will be under Amp selection. We recommend using the low conc. Kan selection and checking your plasmid prep for recombination.
Proper citation: RRID:Addgene_61580 Copy
Species: Homo sapiens
Genetic Insert: rhoA
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7600581
Proper citation: RRID:Addgene_61632 Copy
Species: Staphylococcus aureus
Genetic Insert: hSaCas9
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25830891
Proper citation: RRID:Addgene_61593 Copy
Species: Other
Genetic Insert: hSaCas9
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25830891
Comments: Note: This plasmid does not have an sgRNA expression cassette. Please refer to the associated publication, as well as the AAV portion of http://www.addgene.org/crispr/zhang/ , for more information on expressing sgRNAs.
Proper citation: RRID:Addgene_61594 Copy
Species: Synthetic
Genetic Insert: pHoran4
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25385186
Comments: There is a C terminal Myc tag in frame with pHoran.
Proper citation: RRID:Addgene_61557 Copy
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