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Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3174; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_170416 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3174; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_170413 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3174; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_170414 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3174; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_170412 Copy
Species: E. coli
Genetic Insert: LetA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3174; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_175965 Copy
Species: E. coli
Genetic Insert: LetA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3174; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_175966 Copy
Species: E. coli
Genetic Insert: LetA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3174; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_175968 Copy
Species: E. coli
Genetic Insert: LetA
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:4571; Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_175960 Copy
Species: E. coli
Genetic Insert: LetA
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:4571; Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_175961 Copy
Species: E. coli
Genetic Insert: LetA
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:4571; Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_175962 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3174; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_170425 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3174; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_170421 Copy
Species: E. coli
Genetic Insert: sgNeo3
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35228570
Proper citation: RRID:Addgene_177242 Copy
Species: E. coli
Genetic Insert: LetA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3174; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_175970 Copy
Species: E. coli
Genetic Insert: LetA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3174; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_175972 Copy
Species: E. coli
Genetic Insert: bglR, thi-1, rel-1 HfrPO1, ∆nuoA-N
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:16979134
Comments: Parent strain is 1100, from Humbert et al. 1983 J Bacteriol. doi.org/10.1128/jb.153.1.416-422.1983
Proper citation: RRID:Addgene_186997 Copy
Species: E. coli
Genetic Insert: AraC/PBAD
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:4559; Vector Backbone:pSAM_Ec; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:28614382
Comments: Mariner himar1C9 transposase driven by an arabinose promoter on an R6K gamma suicide plasmid. The transposon allows identification of fitness factors by Illumina sequencing. This transposon will randomly insert a kanamycin resistance cassette into genomic TA sites. Ampicillin resistance is contained on the plasmid backbone.
For further background, see:
Goodman, Andrew L., Meng Wu, and Jeffrey I. Gordon. “Identifying Microbial Fitness Determinants by Insertion Sequencing (INSeq) Using Genome-Wide Transposon Mutant Libraries.” Nature Protocols 6.12 (2011): 1969–1980. PMC. Web. 11 Apr. 2017.
Wiles, Travis J. et al. “Combining Quantitative Genetic Footprinting and Trait Enrichment Analysis to Identify Fitness Determinants of a Bacterial Pathogen.” Ed. Danielle A. Garsin. PLoS Genetics 9.8 (2013): e1003716. PMC. Web. 11 Apr. 2017.
Proper citation: RRID:Addgene_91569 Copy
Species: E. coli
Genetic Insert: FtsZ
Vector Backbone Description: Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18390602
Proper citation: RRID:Addgene_92038 Copy
Species: E. coli
Genetic Insert: ZapA
Vector Backbone Description: Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27310678
Proper citation: RRID:Addgene_92328 Copy
Species: E. coli
Genetic Insert: FtsZ
Vector Backbone Description: Backbone Marker:NBRP-E.coli at NIG; Backbone Size:4500; Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27310678
Proper citation: RRID:Addgene_92329 Copy
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