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Species: Synthetic
Genetic Insert: Leucine Zipper + C-fragment of DiB-RM
Vector Backbone Description: Backbone Size:4504; Vector Backbone:pMRBad; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34748541
Proper citation: RRID:Addgene_168477 Copy
Species: Synthetic
Genetic Insert: C-fragment of DiB-RM
Vector Backbone Description: Backbone Size:4504; Vector Backbone:pMRBad; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34748541
Proper citation: RRID:Addgene_168478 Copy
Species: Synthetic
Genetic Insert: N-fragment of DiB-RM + Leucine Zipper
Vector Backbone Description: Backbone Size:5641; Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34748541
Proper citation: RRID:Addgene_168475 Copy
Species: Synthetic
Genetic Insert: NLS-mEGFP-H-NSdbd-NLS
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:4798; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34734265
Comments: The pcDNA5/FRT/TO backbone has been modified by the addition of a Puromycin resistance gene (Luijsterburg lab, LUMC, Leiden, The Netherlands).
Proper citation: RRID:Addgene_168467 Copy
Species: Synthetic
Genetic Insert: NLS-eYFP-H-NSdbd-NLS
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:4798; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34734265
Comments: The pcDNA5/FRT/TO backbone has been modified by the addition of a Puromycin resistance gene (Luijsterburg lab, LUMC, Leiden, The Netherlands).
Proper citation: RRID:Addgene_168468 Copy
Species: Synthetic
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2812; Vector Backbone:pGem; Vector Types:Cre/Lox, Synthetic Biology, Expression in filamentous fungi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35168324
Comments: For transient Cre recombinase expression in Aspergillus nidulans, we created the helper vector pGem-PgpdA-Cre-TtrpC unable to replicate in filamentous fungi. The helper vector encodes a cassette for constitutive expression of Cre under the promoter gpdA (PgpdA) and the terminator trpC . As the recipient strain A. nidulans LP1 carries nkuA∆, which minimises random integration events, the helper vector presumably would be lost during fungal growth
Proper citation: RRID:Addgene_168784 Copy
Species: Synthetic
Genetic Insert: bar
Vector Backbone Description: Backbone Size:3033; Vector Backbone:pGEM; Vector Types:Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35168324
Comments: Vector used to create Aspergillus nidulans parental strains with LP1 by polyethylene glycol (PEG)-calcium-based transformation with NotI linearized vector pGemLP1-loxP-bar-Lox2272-71 containing 1 kb homology regions at 5′ and 3′ of the floxed bar to facilitate homologous recombination in A. nidulans LO8030 ∆St locus. Colonies are selected for resistance to glufosinate extracted from Basta.
Proper citation: RRID:Addgene_168785 Copy
Species: Synthetic
Genetic Insert: LifeAct-TagBFP-DiB-RM
Vector Backbone Description: Backbone Size:3948; Vector Backbone:pDendra2-N; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34748541
Proper citation: RRID:Addgene_168782 Copy
Species: Synthetic
Genetic Insert: TagBFP - DiB2-split N-frag
Vector Backbone Description: Backbone Size:5296; Vector Backbone:pICH47732; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32632329
Proper citation: RRID:Addgene_168973 Copy
Species: Synthetic
Genetic Insert: mEos3.2_H-NSdbd
Vector Backbone Description: Backbone Size:5625; Vector Backbone:pFPV27; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34734265
Proper citation: RRID:Addgene_168774 Copy
Species: Synthetic
Genetic Insert: mEos3.2
Vector Backbone Description: Backbone Size:5625; Vector Backbone:pFPV27; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34734265
Proper citation: RRID:Addgene_168775 Copy
Species: Synthetic
Genetic Insert: Fluorogen activating protein DiB3
Vector Backbone Description: Backbone Size:3958; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29147545
Proper citation: RRID:Addgene_168802 Copy
Species: Synthetic
Genetic Insert: EGFP , EGFP sgRNA
Vector Backbone Description: Vector Backbone:pRDA_221; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_169142 Copy
Species: Synthetic
Genetic Insert: 3xFlag-6His-nsp15
Vector Backbone Description: Vector Backbone:pBIG1a (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:34198324
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169166 Copy
Species: Synthetic
Genetic Insert: ReaChR-Citrine
Vector Backbone Description: Backbone Marker:Chi-Bin Chien lab; Backbone Size:7796; Vector Backbone:pDestTol2CG2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32516597
Proper citation: RRID:Addgene_169046 Copy
Species: Synthetic
Genetic Insert: 14His-SUMO-nsp10-GGSGGS-nsp14
Vector Backbone Description: Vector Backbone:pK27SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198326
Proper citation: RRID:Addgene_169161 Copy
Species: Synthetic
Genetic Insert: nsp10-6His-3xFlag
Vector Backbone Description: Vector Backbone:pBIG1b (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:34198326
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169162 Copy
Species: Synthetic
Genetic Insert: nsp14/nsp10-6His-3xFlag
Vector Backbone Description: Vector Backbone:pBIG2ab (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:34198326
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169164 Copy
Species: Synthetic
Genetic Insert: 3xFlag-nsp5CS[VRLQ]-nsp10
Vector Backbone Description: Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198326
Comments: We have included the predicted cleavage site for nsp5 found between nsp9 and nsp10 (VRLQ) between the 3xFlag tag and nsp10 in this construct that should allow a purified nsp5 protease to cleave-out the 3xFlag tag similarly to what might happen in cells.
Proper citation: RRID:Addgene_169157 Copy
Species: Synthetic
Genetic Insert: XimD and PcPT
Vector Backbone Description: Vector Backbone:pSE152; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:32786357
Proper citation: RRID:Addgene_169109 Copy
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