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Species: Arabidopsis thaliana
Genetic Insert: GAI
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22446836
Proper citation: RRID:Addgene_37309 Copy
Species: Arabidopsis thaliana
Genetic Insert: BICDN-CIB1
Vector Backbone Description: Backbone Size:3900; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25963241
Proper citation: RRID:Addgene_102253 Copy
Species: Arabidopsis thaliana
Genetic Insert: GFP-BICDN-CIB1
Vector Backbone Description: Backbone Size:3900; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25963241
Proper citation: RRID:Addgene_102254 Copy
Species: Arabidopsis thaliana
Genetic Insert: ABI2
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102383 Copy
Species: Arabidopsis thaliana
Genetic Insert: PBL27
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102388 Copy
Species: Arabidopsis thaliana
Genetic Insert: CBL4
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102396 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIPK5
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102392 Copy
Species: Arabidopsis thaliana
Genetic Insert: CBL1
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102393 Copy
Species: Arabidopsis thaliana
Genetic Insert: CBL9
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102400 Copy
Species: Arabidopsis thaliana
Genetic Insert: RabG3B
Vector Backbone Description: Vector Backbone:pUBN-RFP; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_102371 Copy
Species: Arabidopsis thaliana
Genetic Insert: RabG3B
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102372 Copy
Species: Arabidopsis thaliana
Genetic Insert: EFR
Vector Backbone Description: Vector Backbone:pEarleyGate103; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_102413 Copy
Species: Arabidopsis thaliana
Genetic Insert: Ath-HEN1
Vector Backbone Description: Backbone Size:7517; Vector Backbone:pCFJ350-ttTi5605; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29481550
Proper citation: RRID:Addgene_108362 Copy
Species: Arabidopsis thaliana
Genetic Insert: HDT1
Vector Backbone Description: Vector Backbone:pK7GWIWG2; Vector Types:Plant Expression, RNAi; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28855334
Comments: Full sequence is not available for this plasmid. Please see the diagnostic digest and partial sequencing results as references for verification
To build the pRCH1:HDT1HDT2 RNAi construct (hdt1,2i), the CaMV 35S promoter in pK7GWIWG2 vector (Limpens et al., 2005) was replaced with RCH1 promoter [pK7GWIWG2(II)]. The RCH1 promoter was first amplified on genomic DNA and cloned into pENTR-D-TOPO. Then it was cut out from the vector by HindIII (partial digestion) and XbaI and recombined with two fragments of the pK7GWIWG2(II) vector in a three-point ligation. The two fragments of pK7GWIWG2(II) vector were obtained by digestion either with HindII and NcoI, or with SpeI (compatible with XbaI) and HindIII. The whole RNAi cassette including the RCH1 promoter was cut out from the vector using ApaI and HindIII and ligated into the pBnRGW binary vector digested with the same enzymes and in such way creating pBnRRGWIWG vector. The RNAi target sequences of HDT1 and HDT2 coding sequences (0.6 kb for each) were combined in one amplicon using a two-step PCR, following the procedure described by Franssen et al. (2015). In brief, the first step PCRs introduced short overlaps (15 bp) in PCR fragments of HDT1 and HDT2 coding sequences with HDT1rnai-F and HDT1rnai-R primers, or with HDT2rnai-F and HDT2rnai-R primers. These two fragments were used as templates in the second-step PCR with HDT1rnai-F and HDT2rnai-R primers. The final PCR fragment was introduced into pENTR-D-TOPO vector and recombined in inverse-repeat orientation into the pBnRRGWIWG binary vector by a LR Gateway reaction (Invitrogen).
Proper citation: RRID:Addgene_108446 Copy
Species: Arabidopsis thaliana
Genetic Insert: HDT1
Vector Backbone Description: Vector Backbone:pBnRGW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28855334
Proper citation: RRID:Addgene_108565 Copy
Species: Arabidopsis thaliana
Genetic Insert: TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pAN19; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29355850
Proper citation: RRID:Addgene_108547 Copy
Species: Arabidopsis thaliana
Genetic Insert: RPS5A promoter
Vector Backbone Description: Vector Backbone:pJY-RpABE; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29867128
Comments: Induces Y85H at FT
Proper citation: RRID:Addgene_112874 Copy
Species: Arabidopsis thaliana
Genetic Insert: RPS5A promoter
Vector Backbone Description: Vector Backbone:pKI1.1R; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29867128
Comments: A binary vector for plant adenine base editing through sgRNA cloning using AarI
Proper citation: RRID:Addgene_112872 Copy
Species: Arabidopsis thaliana
Genetic Insert: RPS5A promoter
Vector Backbone Description: Vector Backbone:pJY-RpABE; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29867128
Comments: Induces mis-splicing at PDS3
Proper citation: RRID:Addgene_112875 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtU626-LACZ-sgRNA
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30610635
Proper citation: RRID:Addgene_112909 Copy
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