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Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161898 Copy
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161897 Copy
Vector Backbone Description: Vector Backbone:PB-TAG-ERN; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33080218
Proper citation: RRID:Addgene_164521 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmec-4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5020; Vector Backbone:Gateway Destination Vector; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20203177
Proper citation: RRID:Addgene_110883 Copy
Vector Backbone Description: Backbone Marker:Vidali Lab; Backbone Size:11239; Vector Backbone:pGAPi; Vector Types:RNAi; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:32764132
Proper citation: RRID:Addgene_127547 Copy
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_128322 Copy
Species: Aequorea victoria
Genetic Insert: Venus
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:7470; Vector Backbone:pDEST15; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27455993
Comments: 5' cloning site: AgeI (unknown if destroyed), 3' cloning site: MluI (unknown if destroyed)
Proper citation: RRID:Addgene_128567 Copy
Species: Aequorea victoria
Genetic Insert: Venus
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:7470; Vector Backbone:pDEST15; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27455993
Comments: 5' cloning site: AgeI (unknown if destroyed), 3' cloning site: MluI (unknown if destroyed)
Proper citation: RRID:Addgene_128566 Copy
Genetic Insert: cat, sacB
Vector Backbone Description: Backbone Size:0; Vector Backbone:pTP809; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:10767554
Comments: pKM154 is a derivative of pBR322 that had sequence between the EcoR1 site and NdeI site removed, with a NotI site inserted (pTP809).
Proper citation: RRID:Addgene_13036 Copy
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:5778; Vector Backbone:na; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_13071 Copy
Genetic Insert: 6x Myc Tag
Vector Backbone Description: Backbone Size:5818; Vector Backbone:na; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_13070 Copy
Vector Backbone Description: Backbone Marker:Gregory Newby; Backbone Size:10365; Vector Backbone:pGAN049; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29056345
Comments: This is one of the empty dual yTRAP sensor plasmids. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pSK247. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the LEU2 locus, and can be selected for using 100 ug/ml G418. It can be used in combination with pSK260.
Proper citation: RRID:Addgene_131148 Copy
Vector Backbone Description: Backbone Marker:Szilvia Kiriakov; Backbone Size:10377; Vector Backbone:pSK247; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29056345
Comments: This is the empty dual yTRAP sensor plasmid. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pSK234. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the LEU2 locus, and can be selected for using 100 ug/ml G418. It can be used in combination with an expression clone resulting from LR reaction using pGAN147 basic yTRAP sensor.
Proper citation: RRID:Addgene_131149 Copy
Vector Backbone Description: Backbone Marker:Gregory Newby; Backbone Size:11381; Vector Backbone:pGAN147; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29056345
Comments: This is the empty reverse yTRAP sensor plasmid. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pGAN230. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the HO locus, and can be selected for using nourseothricin. To achieve dual color reporting, combine with pSK221.
Proper citation: RRID:Addgene_131122 Copy
Genetic Insert: Hygromycin resistance
Vector Backbone Description: Backbone Size:7654; Vector Backbone:pCFJ201; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31378567
Proper citation: RRID:Addgene_135096 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7892; Vector Backbone:pHTN HaloTag CMV-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30217970
Proper citation: RRID:Addgene_136403 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7662; Vector Backbone:pNLF1-N CMV-hygro; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30217970
Proper citation: RRID:Addgene_136404 Copy
Genetic Insert: Gateway cassette
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4720; Vector Backbone:pQE-2; Vector Types:Bacterial Expression, Co-Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17311810
Comments: Please note that the chloramphenicol resistance gene outside of the attB sites in this plasmid is incomplete. It is lacking a promoter, therefore it is supposed to be inactive.
Proper citation: RRID:Addgene_13668 Copy
Genetic Insert: Gateway cassette
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:5356; Vector Backbone:pQE-2; Vector Types:Bacterial Expression, Co-Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17311810
Comments: Please note that the chloramphenicol resistance gene outside the attB sites in this plasmid is incomplete. It is lacking a promoter, therefore it is supposed to be inactive.
Proper citation: RRID:Addgene_13673 Copy
Vector Backbone Description: Backbone Size:11698; Vector Backbone:pCR8/GW/TOPO; Vector Types:Mammalian Expression, Other, Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134975 Copy
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