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Species: E. coli
Genetic Insert: FtsZ
Vector Backbone Description: Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27310678
Proper citation: RRID:Addgene_92327 Copy
Species: E. coli
Genetic Insert: MscL
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_92418 Copy
Species: E. coli
Genetic Insert: LacZalpha
Vector Backbone Description: Backbone Marker:Alexeyev lab; Backbone Size:3008; Vector Backbone:pMA3735; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35883613
Proper citation: RRID:Addgene_184848 Copy
Species: E. coli
Genetic Insert: anti rabbit secondary nanobody fused to Tn5
Vector Backbone Description: Vector Backbone:pET-29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36536148
Comments: Please visit https://doi.org/10.1101/2022.03.08.483459 for bioRxiv preprint.
Proper citation: RRID:Addgene_183638 Copy
Species: E. coli
Genetic Insert: Hfq
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29140461
Proper citation: RRID:Addgene_174661 Copy
Species: E. coli
Genetic Insert: rpoD
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Addgene NGS found a N149D amino acid variation in the rpoD translation, that the depositing laboratory confirms to be a naturally occurring sequence variant. This will not adversely affect plasmid function.
Please visit https://www.biorxiv.org/content/10.1101/2020.02.17.953182v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_129688 Copy
Species: E. coli
Genetic Insert: codon optimized S1 subunit of OZITX
Vector Backbone Description: Vector Backbone:pcDNA3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35322196
Proper citation: RRID:Addgene_184925 Copy
Species: E. coli
Genetic Insert: Scrambled sgRNA targeting sequences
Vector Backbone Description: Vector Backbone:L4440_BioBrick-sgRNA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35636511
Proper citation: RRID:Addgene_177811 Copy
Species: E. coli
Genetic Insert: None
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:31772280
Comments: Genotype: λDE3 Δtig100 Δ(araD–araB)567 ΔlacZ4787(::rrnB-3) lacIP-4000(lacIQ) rph-1 Δ(rhaD–rhaB)568 hsdR514
KTD101(DE3) is a λDE3 derivative of the trigger factor deficient (Δtig) strain KTD101 from Puertas et al 2010 Protein Expression and Purification 74: 122–128, PMID 20600941.
Puertas et al 2010 showed that deletion of trigger factor in the parent strain KTD101 increased the level of secreted recombinantly expressed protein. This strain may also improve the level of protein expression in the periplasm and in the outer membrane. This derivative strain KTD101(DE3) now allows expression that is based on the widely-used T7 promoter.
The parent strain KTD101 was provided by François Baneyx at the University of Washington.
DE3 lysogenization is apparent from positive expression that was obtained for the BBA57 protein upon expression from the T7 promoter plasmid pBBA57-TEV-His12, as was shown in Figure S5C, lane 7, of Robertson et al, Sci Rep. 2019 Nov 26;9(1):17606. doi: 10.1038/s41598-019-53830-x.
Proper citation: RRID:Addgene_138651 Copy
Species: E. coli
Genetic Insert: PPK
Vector Backbone Description: Vector Backbone:p416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35862758
Proper citation: RRID:Addgene_183943 Copy
Species: E. coli
Genetic Insert: PPK
Vector Backbone Description: Vector Backbone:pRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35862758
Proper citation: RRID:Addgene_183941 Copy
Species: E. coli
Genetic Insert: FtsZ
Vector Backbone Description: Vector Backbone:pDR175; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25848771
Proper citation: RRID:Addgene_98913 Copy
Species: E. coli
Genetic Insert: FtsZ
Vector Backbone Description: Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25848771
Proper citation: RRID:Addgene_98914 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91228 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91245 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91241 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91239 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91237 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91235 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91234 Copy
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