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Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25748654
Comments: Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check https://zlab.bio/ for the most up-to-date information.
Proper citation: RRID:Addgene_63592 Copy
Species: Leishmania
Genetic Insert: guide sequence
Vector Backbone Description: Vector Backbone:pSPneo; Vector Types:Leishmania Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199327
Comments: The guide sequence will be cloned into the two Bbs I sites ( See attached map). This plasmid contains secondary structure and needs to be sequenced with a modified protocol.
Proper citation: RRID:Addgene_63556 Copy
Species: Leishmania
Genetic Insert: guide sequence
Vector Backbone Description: Vector Backbone:pSPneo; Vector Types:Leishmania Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199327
Comments: The guide sequence will be cloned into the two Bbs I sites (See attached map). This plasmid contains secondary structure and needs to be sequenced with a modified protocol.
Proper citation: RRID:Addgene_63557 Copy
Species: S. pyogenes and F. okeanokoites
Genetic Insert: FokI-dCas9
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25997509
Proper citation: RRID:Addgene_63603 Copy
Species: S. pyogenes and F. okeanokoites
Genetic Insert: FokI-dCas9
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25997509
Proper citation: RRID:Addgene_63604 Copy
Species: S. pyogenes and F. okeanokoites
Genetic Insert: FokI-dCas9
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25997509
Proper citation: RRID:Addgene_63606 Copy
Species: Homo sapiens
Genetic Insert: H2A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22980979
Proper citation: RRID:Addgene_63566 Copy
Species: Homo sapiens
Genetic Insert: H2A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22980979
Proper citation: RRID:Addgene_63567 Copy
Species: Homo sapiens
Genetic Insert: H2A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22980979
Proper citation: RRID:Addgene_63568 Copy
Species: S. pyogenes
Genetic Insert: humanized S. pyogenes dCas9
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25997509
Proper citation: RRID:Addgene_63601 Copy
Species: Mus musculus
Genetic Insert: Bicaudal D protein
Vector Backbone Description: Backbone Size:7041; Vector Backbone:pBa-eGFP-FKBP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25624392
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.
Proper citation: RRID:Addgene_63569 Copy
Species: S. pyogenes and F. okeanokoites
Genetic Insert: FokI-dCas9
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25997509
Proper citation: RRID:Addgene_63602 Copy
Species: Synthetic
Genetic Insert: Stop-Lox71-Luciferase-pA-FRT-Lox66-reverseCAG
Vector Backbone Description: Backbone Size:1960; Vector Backbone:pUC; Vector Types:Mammalian Expression, Cre/Lox, FRT/Flpe recombination mediated insertion; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26492136
Proper citation: RRID:Addgene_63577 Copy
Species: Homo sapiens
Genetic Insert: USP7
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21981925
Comments: Please note that the USP7 gene in this plasmid contains mutations S600P, F679L and V1020A when compared to the annotated gene/protein sequences within the Genbank/Uniprot databases. This plasmid should function as described in the associated publication.
Proper citation: RRID:Addgene_63573 Copy
Species: Mus musculus
Genetic Insert: Col1A-frt-hygro-pA
Vector Backbone Description: Backbone Size:2888; Vector Backbone:pBluescriptII SK; Vector Types:Mammalian Expression, Bacterial Expression, Mouse Targeting, FRT/Flpe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26492136
Comments: The cassette in between the homologous recombination arms is the same as in AddGene Plasmid #20730.
The homologous recombination arms are derived from mouse strain FVB.
The Hygromycin gene in this construct does not have an ATG start codon nor is it expressed. Hygromycin can not be used for selection when this construct is used in a targeting experiment in mouse strain FVB derived ES cells.
The construct can be linearized with AscI, FseI or XhoI
Proper citation: RRID:Addgene_63575 Copy
Species: Homo sapiens
Genetic Insert: UBA1
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15723079
Proper citation: RRID:Addgene_63571 Copy
Species: Homo sapiens
Genetic Insert: UBE2-25K
Vector Backbone Description: Vector Backbone:pGEX4T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15723079
Proper citation: RRID:Addgene_63572 Copy
Genetic Insert: VfAU1-LOV
Vector Backbone Description: Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63632 Copy
Species: Drosophila melanogaster
Genetic Insert: TGF-β activated kinase 1
Vector Backbone Description: Backbone Marker:lifetechnologies; Backbone Size:3500; Vector Backbone:pMT-V5-His; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63753 Copy
Species: Other
Genetic Insert: EAggEC
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2189007
Comments: DNA probe for the identification of enteroaggregative E. coli
Proper citation: RRID:Addgene_63755 Copy
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