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  • Bacterial Resistance:ampicillin (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pHIS2-KL EAT
 
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RRID:Addgene_46042 Atg1 EAT domain K. lactis Ampicillin PMID:23219485 Backbone Size:5550; Vector Backbone:pHIS-parallel.2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:28 0
pMM287
 
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RRID:Addgene_46035 WRN K577M Homo sapiens Ampicillin PMID:15084309 Bidirectional vector expresses WRN and GFP at similar expression levels. In the associated article, cells were transiently transfected with this plasmid together with a tTA transactivator plasmid (Gossen, 1992). This is a mammalian expression vector for helicase-dead human WRN protein. It was constructed from pBI, a Clontech plasmid with a bi-directional promoter+ TRE backbone, modified by insertion of a polylinker containing BspD1 and Xhol sites followed by insertion of a myc-WRN K577M ORF- created by making K577M (A>T) mutation in pMM290 sequence. The S2G mutation was a result of the cloning strategy to add the myc tag. The WRN protein containing S2G was biochemically verified and is wild-type in terms of activities, as described in the associated publication. Backbone Marker:Clontech; Vector Backbone:pBI; Vector Types:Mammalian Expression, TetOn, bidirectional promoter; Bacterial Resistance:Ampicillin K577M helicase-dead mutant; S2G introduced during cloning 2026-08-15 01:15:28 0
tetO-GATA4
 
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RRID:Addgene_46030 GATA4 Homo sapiens Ampicillin PMID:23591016 Please note that Addgene's sequencing identified R43Q, G50S and N197S mutations in the GATA4 insert in this plasmid when compared to GenBank reference sequence NP_002043.2. These difference were present in the original GATA4 sample and the plasmid functions as described in the associated publication. Vector Backbone:tetO-Gateway; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin R43Q, G50S and N197S 2026-08-15 01:15:28 6
tetO-MEF2C
 
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RRID:Addgene_46031 MEF2C Homo sapiens Ampicillin PMID:23591016 Vector Backbone:tetO-Gateway; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:15:28 4
pE-FLP
 
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10+ mentions
RRID:Addgene_45978 flp Ampicillin PMID:24050148 Can be used to remove the integration module (including the antibiotic resistance cassette) from pOSIP integrants. Reference: St-Pierre F, Cui L et al., "One-step cloning and chromosomal integration of DNA", ACS Synthetic Biology, http://pubs.acs.org/doi/abs/10.1021/sb400021j Vector Backbone:pKD46; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin flp driven by the constitutive promoter pE from phage P2 2026-08-15 01:15:28 15
pGEX-parkin W403A-C431S
 
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RRID:Addgene_45977 Parkin W403A-C431S Rattus norvegicus Ampicillin PMID:23661642 Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Codon usage optimized for E.coli expression. Double mutation W403A-C431S 2026-08-15 01:15:28 0
pGEX-parkin 219-465
 
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RRID:Addgene_45972 Parkin 219-465 Rattus norvegicus Ampicillin PMID:23661642 Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Codon usage optimized for E.coli expression. Deleted a.a. 1-219 2026-08-15 01:15:28 0
CMV-O-GECO1
 
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1+ mentions
RRID:Addgene_46025 O-GECO1 Synthetic Ampicillin PMID:23452507 Backbone Size:3200; Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin R-GECO1 N45I/A73V/S142P/M146R/M223T/M339L/K378R/E386V/K397N 2026-08-15 01:15:28 2
pGEX-parkin F146A
 
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RRID:Addgene_45973 Parkin F146A Rattus norvegicus Ampicillin PMID:23661642 Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Codon usage optimized for E.coli expression. Changed Phe146 to alanine. 2026-08-15 01:15:28 0
tetO-Hand2
 
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1+ mentions
RRID:Addgene_46028 Hand2 Mus musculus Ampicillin PMID:23591016 Vector Backbone:tetO-Gateway; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:15:28 1
pGEX-parkin F463A
 
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RRID:Addgene_45975 Parkin F463A Rattus norvegicus Ampicillin PMID:23661642 Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Codon usage optimized for E.coli expression. Changed Phe463 to alanine. 2026-08-15 01:15:28 0
CMV-mito-CAR-GECO1
 
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10+ mentions
RRID:Addgene_46022 CAR-GECO1 Synthetic Ampicillin PMID:23452507 The vector of the CMV-mito-CAR-GECO1 is based on the mitATeam1.03 pcDNA plasmid reported here: (http://www.pnas.org/content/106/37/15651.abstract). Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1 (-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P 2026-08-15 01:15:28 14
CMV-mito-R-GECO1
 
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RRID:Addgene_46021 R-GECO1 Synthetic Ampicillin PMID:23452507 The vector of the CMV-mito-R-GECO1 is based on the mitATeam1.03 pcDNA plasmid reported here: (http://www.pnas.org/content/106/37/15651.abstract). Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1 (-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Substitutions relative to the mApple-derived analogue of GCaMP3: T47A/L60P/E61V/S63V/E64S/R81G/K83R/Y134C/M158L/N164aD/V228A/ S290P/I366F/K380N/S404G/N414D/E430V 2026-08-15 01:15:28 22
pGEX-parkin WT
 
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RRID:Addgene_45969 Parkin Rattus norvegicus Ampicillin PMID:23661642 Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Codon usage optimized for E.coli expression 2026-08-15 01:15:28 0
pCMV-Luc
 
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1+ mentions
RRID:Addgene_45968 Luciferase Firefly Ampicillin Backbone Marker:Promega; Vector Backbone:pGL3 basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:27 8
pUAST YFP mouse Rab35 DN
 
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RRID:Addgene_46016 Rab35 Mus musculus Ampicillin PMID:19729655 Backbone Size:8900; Vector Backbone:pUAST; Vector Types:Insect Expression, Drosophila; Bacterial Resistance:Ampicillin S22N (dominant negative) 2026-08-15 01:15:28 0
pCM351
 
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RRID:Addgene_46013 Ampicillin PMID:12449384 This allelic exchange vector is used with Cre expression vectors (Addgene plasmids 45863 or 45864) to generate unmarked mutants in a broad bacterial host range. Backbone Marker:Lidstrom Lab (PMID 8021187); Backbone Size:6000; Vector Backbone:pAYC61; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin 2026-08-15 01:15:28 0
pUAST YFP mouse Rab35 WT
 
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RRID:Addgene_46014 Rab35 Mus musculus Ampicillin PMID:19729655 Backbone Size:8900; Vector Backbone:pUAST; Vector Types:Insect Expression, Drosophila; Bacterial Resistance:Ampicillin 2026-08-15 01:15:28 0
pCAG-TALEN-G-pA
 
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RRID:Addgene_45962 TALEN Synthetic Ampicillin Backbone Size:2500; Vector Backbone:pBluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:27 0
pUASP YFP Rab9Fb WT
 
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RRID:Addgene_46017 Rab9Fb Drosophila melanogaster Ampicillin PMID:17409086 Backbone Size:9939; Vector Backbone:pUASP; Vector Types:Insect Expression, Drosophila; Bacterial Resistance:Ampicillin 2026-08-15 01:15:28 0

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