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Species: Synthetic
Genetic Insert: protein G - NanoLuc
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31909607
Proper citation: RRID:Addgene_169105 Copy
Species: Synthetic
Genetic Insert: protein G - mNeonGreen - NanoLuc
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31909607
Proper citation: RRID:Addgene_169106 Copy
Species: Synthetic
Genetic Insert: nsp7
Vector Backbone Description: Vector Backbone:pBIG1b (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:34198323
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169178 Copy
Species: Synthetic
Genetic Insert: nsp8
Vector Backbone Description: Vector Backbone:pBIG1c (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:34198323
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169179 Copy
Species: Synthetic
Genetic Insert: nsp7-Linker-nsp8
Vector Backbone Description: Vector Backbone:pBIG1b (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:34198323
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169181 Copy
Species: Synthetic
Genetic Insert: 6xHis mCherry F30-Broccoli
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2665; Vector Backbone:pEXP5-NT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35057626
Comments: Plasmid used for monitoring transcription and translation dynamics. Broccoli aptamer provides a fluorescent signal with DFHBI for mRNA level and mCherry provides fluorescent signal for protein level. Design and sequence of the Broccoli aptamer is from Filonov et al. (2015, doi: 10.1016/j.chembiol.2015.04.018).
Proper citation: RRID:Addgene_169234 Copy
Species: Synthetic
Genetic Insert: 3xFlag-His6-PLpro
Vector Backbone Description: Vector Backbone:pLIB (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198325
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169194 Copy
Species: Synthetic
Genetic Insert: GST-PLproCS-MBP
Vector Backbone Description: Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198325
Proper citation: RRID:Addgene_169195 Copy
Species: Synthetic
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:SIN40C; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34570885
Proper citation: RRID:Addgene_169307 Copy
Species: Synthetic
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:SIN40C; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34570885
Proper citation: RRID:Addgene_169308 Copy
Species: Synthetic
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:SIN40C; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34570885
Proper citation: RRID:Addgene_169317 Copy
Species: Synthetic
Genetic Insert: Double SapI flanked mNeongreen-P2A-Puro
Vector Backbone Description: Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35089911
Proper citation: RRID:Addgene_169228 Copy
Species: Synthetic
Genetic Insert: Double SapI flanked mTurquoise2-P2A-Puro
Vector Backbone Description: Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35089911
Proper citation: RRID:Addgene_169224 Copy
Species: Synthetic
Genetic Insert: Double SapI flanked mTurquoise2-P2A-Hygro
Vector Backbone Description: Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35089911
Proper citation: RRID:Addgene_169225 Copy
Species: Synthetic
Genetic Insert: Double SapI flanked mNeongreen
Vector Backbone Description: Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35089911
Proper citation: RRID:Addgene_169226 Copy
Species: Synthetic
Genetic Insert: 14His-SUMO-nsp12
Vector Backbone Description: Vector Backbone:pK27Sumo; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198323
Proper citation: RRID:Addgene_169188 Copy
Species: Synthetic
Genetic Insert: Double SapI flanked mScarlet-P2A-Blast
Vector Backbone Description: Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35089911
Proper citation: RRID:Addgene_169221 Copy
Species: Synthetic
Genetic Insert: 3xFlag-His6-nsp13
Vector Backbone Description: Vector Backbone:pLIB (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198322
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169189 Copy
Species: Synthetic
Genetic Insert: Double SapI flanked mTurquoise2
Vector Backbone Description: Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35089911
Proper citation: RRID:Addgene_169222 Copy
Species: Synthetic
Genetic Insert: nsp12-His6-3xFlag/nsp7-Linker-nsp8
Vector Backbone Description: Vector Backbone:pBIG2abc (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:34198323
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169184 Copy
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