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Species: Synthetic
Genetic Insert: nsp12-His6-3xFlag/nsp7/nsp8
Vector Backbone Description: Vector Backbone:pBIG2abc (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:34198323
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169185 Copy
Species: Synthetic
Genetic Insert: 14His-SUMO-nsp10-GSGSGS-nsp16
Vector Backbone Description: Vector Backbone:pK27SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198328
Proper citation: RRID:Addgene_169191 Copy
Species: Synthetic
Genetic Insert: 14His-SUMO-nsp3_E746-K1060 (pp1ab_E1564-K1878)
Vector Backbone Description: Vector Backbone:pK27SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198325
Proper citation: RRID:Addgene_169193 Copy
Species: Synthetic
Genetic Insert: Double SapI flanked Dendra2-P2A-Puro
Vector Backbone Description: Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35089911
Proper citation: RRID:Addgene_169217 Copy
Species: Synthetic
Genetic Insert: Double SapI flanked mScarlet
Vector Backbone Description: Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35089911
Proper citation: RRID:Addgene_169218 Copy
Species: Synthetic
Genetic Insert: Double SapI flanked mScarlet
Vector Backbone Description: Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35089911
Proper citation: RRID:Addgene_169219 Copy
Species: Synthetic
Genetic Insert: GFP-Cre recombinase
Vector Backbone Description: Backbone Size:4561; Vector Backbone:pAAV hSyn; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_175381 Copy
Species: Synthetic
Genetic Insert: TVA-P2A-eGFP-P2A-N2cG
Vector Backbone Description: Backbone Marker:Jordane Dimidschstein (Addgene plasmid # 170856); Vector Backbone:pAAV-VTKS5; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34758329
Proper citation: RRID:Addgene_175440 Copy
Species: Synthetic
Genetic Insert: TVA-P2A-eGFP-P2A-N2cG
Vector Backbone Description: Backbone Marker:Jordane Dimidschstein (Addgene plasmid # 170853); Vector Backbone:pAAV-VTKS2; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34758329
Proper citation: RRID:Addgene_175439 Copy
Species: Synthetic
Genetic Insert: tcTRP24sub8_SH2sub6
Vector Backbone Description: Backbone Marker:Stoddard Lab; Backbone Size:5727; Vector Backbone:pET15HE; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34716407
Proper citation: RRID:Addgene_175799 Copy
Species: Synthetic
Genetic Insert: tcTRP9
Vector Backbone Description: Backbone Marker:Stoddard Lab; Backbone Size:5727; Vector Backbone:pET15HE; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34716407
Proper citation: RRID:Addgene_175793 Copy
Species: Synthetic
Genetic Insert: tcTRP24sub6
Vector Backbone Description: Backbone Marker:Stoddard Lab; Backbone Size:5727; Vector Backbone:pET15HE; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34716407
Proper citation: RRID:Addgene_175795 Copy
Species: Synthetic
Genetic Insert: tcTRP24sub8
Vector Backbone Description: Backbone Marker:Stoddard Lab; Backbone Size:5727; Vector Backbone:pET15HE; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34716407
Proper citation: RRID:Addgene_175796 Copy
Species: Synthetic
Genetic Insert: tcTRP24sub8_Cap
Vector Backbone Description: Backbone Marker:Stoddard Lab; Backbone Size:5727; Vector Backbone:pET15HE; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34716407
Proper citation: RRID:Addgene_175797 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176016 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34633413
Proper citation: RRID:Addgene_176015 Copy
Species: Synthetic
Genetic Insert: LifeAct
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_176105 Copy
Species: Synthetic
Genetic Insert: iLID
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37449837
Comments: Please visit https://doi.org/10.1101/2022.10.17.512253 for bioRxiv preprint.
Proper citation: RRID:Addgene_176125 Copy
Species: Synthetic
Genetic Insert: SR6v15
Vector Backbone Description: Backbone Size:5639; Vector Backbone:pETx; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34535642
Proper citation: RRID:Addgene_176212 Copy
Species: Synthetic
Genetic Insert: 41 bp non-coding DNA spacer from pYTK048
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015).
Proper citation: RRID:Addgene_176178 Copy
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