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Species: Caenorhabditis elegans
Genetic Insert: hlh-12_genomic region
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:0; Vector Backbone:pCR-XL-TOPO; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19688 Copy
Species: Caenorhabditis elegans
Genetic Insert: <1xNLS-lacZ::unc-54(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pPD95.03; Vector Types:Worm Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19654 Copy
Species: Caenorhabditis elegans
Genetic Insert:
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19656 Copy
Species: Caenorhabditis elegans
Genetic Insert: <1xNLS-lacZ::unc-54(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pPD95.03; Vector Types:Worm Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19655 Copy
Species: Caenorhabditis elegans
Genetic Insert: <*iA-1xNLS-DsRed2::let-858(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC293; Vector Types:Worm Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19658 Copy
Species: Caenorhabditis elegans
Genetic Insert: <*iA-4xNLS-DsRed2::let-858(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC292; Vector Types:Worm Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19657 Copy
Species: Caenorhabditis elegans
Genetic Insert: <*-DsRed2::let-858(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC306; Vector Types:Worm Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19659 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pceh-22::tdimer2(12)::let-858(3')
Vector Backbone Description: Backbone Size:0; Vector Backbone:pPD117.01; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19709 Copy
Species: Caenorhabditis elegans
Genetic Insert: <*-CFP::let-858(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC307; Vector Types:Worm Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19663 Copy
Species: Caenorhabditis elegans
Genetic Insert: <*iA-1xNLS-CFP::let-858(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC294; Vector Types:Worm Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19662 Copy
Species: Caenorhabditis elegans
Genetic Insert: <*-YFP::let-858(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC308; Vector Types:Worm Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19667 Copy
Species: Caenorhabditis elegans
Genetic Insert: <*iA-1xNLS-YFP::let-858(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC295; Vector Types:Worm Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19666 Copy
Species: Caenorhabditis elegans
Genetic Insert: <*iA-4xNLS-mCherry::let-858(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC292; Vector Types:Worm Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19668 Copy
Species: Caenorhabditis elegans
Genetic Insert: lsm-1
Vector Backbone Description: Backbone Size:0; Vector Backbone:unknown; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18692039
Proper citation: RRID:Addgene_26939 Copy
Species: Caenorhabditis elegans
Genetic Insert: cgh-1
Vector Backbone Description: Backbone Size:0; Vector Backbone:unknown; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18692039
Proper citation: RRID:Addgene_26940 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119 rescuing fragment/pie-1promoter-gateway destination cassette B- pie-1 3’
Vector Backbone Description: Backbone Size:17037; Vector Backbone:pie-1; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18692039
Comments: Second Generation pie-1 vector for expressing ORFs in the germline
Proper citation: RRID:Addgene_26955 Copy
Species: Caenorhabditis elegans
Genetic Insert: beta-tubulin
Vector Backbone Description: Backbone Size:0; Vector Backbone:pie-1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18692039
Comments: 1st generation pie-1 vector
Proper citation: RRID:Addgene_26951 Copy
Species: Caenorhabditis elegans
Genetic Insert: TRA-2 GFP
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pPD95.79; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20921392
Proper citation: RRID:Addgene_27128 Copy
Species: Caenorhabditis elegans
Genetic Insert: TRA-2 FLAG
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pPD95.79; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20921392
Comments: Cloning of this plasmid was achieved in the following steps:
(1)the sequence 3' (3'UTR) of the tags was added by (EcoRI/SpeI)
(2) 1.2 kb XhoI/KpnI tra-2 pcr fragment into SalI/KpnI cut vector (destroying one SalI/XhoI site but adding another in the insert) immediately 5' of the tag
(3) 7 kb HindIII/SalI fragment into same that partially replaced fragment added in step 2 containing most of the TRA-2 coding sequence
(4) 5 kb Xho/AgeI that added the promoter and upstream gene in the operon as well as some Tra-2 coding sequence
Proper citation: RRID:Addgene_27129 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmyo-3::cfp::lacZ::unc-54_3'UTR
Vector Backbone Description: Backbone Size:2000; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20493184
Comments: Please note that there are some slight discrepancies between Addgene's quality control sequence and the theoretical sequence from the Fire lab. These differences should not affect function.
Proper citation: RRID:Addgene_27510 Copy
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