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Species: Other
Genetic Insert: HCoV-HKU1-nsp9
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168898 Copy
Species: Other
Genetic Insert: HCoV-HKU1-nsp10
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168899 Copy
Species: Other
Genetic Insert: HCoV-NL63-nsp3
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168930 Copy
Species: Other
Genetic Insert: HCoV-OC43-nsp14
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168939 Copy
Species: Other
Genetic Insert: HCoV-OC43-nsp7
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168935 Copy
Species: Other
Genetic Insert: HCoV-OC43-nsp8
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168936 Copy
Species: Other
Genetic Insert: HCoV-NL63-nsp14
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168920 Copy
Species: Other
Genetic Insert: HCoV-NL63-nsp15
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168921 Copy
Species: Other
Genetic Insert: HCoV-NL63-nsp16
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Please note that a transposon was detected in the plasmid backbone. The plasmid is still useful for gateway cloning. Please see the Addgene NGS result for reference.
Proper citation: RRID:Addgene_168922 Copy
Species: Other
Genetic Insert: HCoV-NL63-nsp13
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168928 Copy
Species: Other
Genetic Insert: HCoV-NL63-Spike
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168929 Copy
Species: Other
Genetic Insert: HCoV-NL63-Membrane
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168925 Copy
Species: Other
Genetic Insert: HCoV-NL63-Nucleocapsid
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168926 Copy
Species: Candidatus Methanomethylophilus alvus
Genetic Insert: Pyrrolysyl-tRNA synthetase
Vector Backbone Description: Backbone Marker:Peter Schultz; Backbone Size:4029; Vector Backbone:pUltra; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37264106
Proper citation: RRID:Addgene_200225 Copy
Species: Other
Genetic Insert: RcaT-Sen2 toxin
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38724726
Proper citation: RRID:Addgene_202602 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: tsi5
Vector Backbone Description: Backbone Marker:de Lorenzo Lab (https://vdl-lab.com/); Vector Backbone:pSEVA424; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36335275
Comments: Please visit https://doi.org/10.21203/rs.3.rs-967174/v1 for preprint.
Proper citation: RRID:Addgene_192960 Copy
Species: Synthetic
Genetic Insert: cCadC-GCN4
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36215660
Proper citation: RRID:Addgene_194800 Copy
Genetic Insert: cCadC-GCN4*
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36215660
Proper citation: RRID:Addgene_194802 Copy
Genetic Insert: Mphot
Vector Backbone Description: Vector Backbone:pMpGE010; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29885839
Proper citation: RRID:Addgene_186728 Copy
Species: F. Novicida
Genetic Insert: Cas4, Cas1 and Cas2
Vector Backbone Description: Vector Backbone:pY002; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35833800
Proper citation: RRID:Addgene_186439 Copy
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