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Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::lys1-W151N-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210186 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::lys1-W151K-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210184 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::ade1-G54R-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210180 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::lys1-W151V-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210163 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::aro7-D147G-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210168 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::aro7-G141S-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210178 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::lys1-W353G-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210181 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::lys1-I36D-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210162 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::aro7-D147V-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210169 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::lys1-I245N-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210166 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::aro7-R33G-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210167 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YIp ADE2-LEU2 pTDH3::aro7-R33P-GFP::tADH1
Vector Backbone Description: Vector Backbone:pRH3022; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37729018
Proper citation: RRID:Addgene_210187 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Residues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension
Vector Backbone Description: Vector Backbone:pet3b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2145515
Comments: Dynamics of GCN4 facilitate DNA interaction: a model-free analysis of an intrinsically disordered region. Gill ML, Byrd RA, Palmer AG III. Phys Chem Chem Phys. 2016 Feb 17;18(8):5839-49. doi: 10.1039/c5cp06197k.
The GCN4 basic region leucine zipper binds DNA as a dimer of uninterrupted a-helices: crystal structure of the protein-DNA complex. Ellenberger, T. E., Brandl, C. J., Struhl, K. & Harrison, S. C. (1992). Cell, 71, 1223-1237. PMID 1473154.
Proper citation: RRID:Addgene_202461 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLP recombinase
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35688132
Comments: pEHA1338
Proper citation: RRID:Addgene_209087 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MDHM_YEAST
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5320; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: UniProt ID: https://www.uniprot.org/uniprotkb/P17505/.
Expression changes based on metabolite starting point. Involvement of MDH in the asp-malate shuttle in yeast extends replicative lifespan and may play a role in calorie-restricted life extension.
For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/.
Proper citation: RRID:Addgene_204231 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MDHP_YEAST
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5235; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: UniProt ID: https://www.uniprot.org/uniprotkb/P32419/.
The affinity of MDH3 for OAA is lower than those of MDH1 and MDH2. The structure of the ternary complex suggests that the active-site loop is in the open conformation, differing from the closed conformations in mitochondrial and cytosolic malate dehydrogenases.
For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/.
Proper citation: RRID:Addgene_204233 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPO41
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21454631
Proper citation: RRID:Addgene_205076 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tHMGR
Vector Backbone Description: Vector Backbone:pLRD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_208259 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tHMGR
Vector Backbone Description: Vector Backbone:pLRD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_208260 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tHMGR
Vector Backbone Description: Vector Backbone:pLRD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_208263 Copy
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