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Species: Caenorhabditis elegans
Genetic Insert: pes-10delta::SV40 NLS::mScarlet_I::PEST(dpi)::-tbb-2 3'UTR
Vector Backbone Description: Backbone Size:2360; Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33677541
Proper citation: RRID:Addgene_154340 Copy
Species: Caenorhabditis elegans
Genetic Insert: Peft-3::wrmScarlet::tbb-2 UTR
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32816924
Comments: Protocols, sequence information, and updates can be found at: https://github.com/phillips-lab/SLP
Proper citation: RRID:Addgene_154824 Copy
Species: Caenorhabditis elegans
Genetic Insert: synthetic guide::∆HYGR::unc-54 3' UTR
Vector Backbone Description: Backbone Size:10683; Vector Backbone:pMS4; Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32816924
Comments: Additional protocol information can be found at: github.com/phillips-lab/SLP
Proper citation: RRID:Addgene_154838 Copy
Species: Caenorhabditis elegans
Genetic Insert: homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
Vector Backbone Description: Backbone Size:2470; Vector Backbone:pUC57; Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32816924
Comments: The LoxP site has a point mutation that in our hands does not noticeably impact function.
The rpl-28p::mKate2::unc-54 3'UTR transgene is flanked by restriction sites to facilitate removal and replacement of this gene.
Additional protocol information can be found at: github.com/phillips-lab/SLP
Proper citation: RRID:Addgene_154840 Copy
Species: Caenorhabditis elegans
Genetic Insert: wrmScarlet11 x3 tandem repeats
Vector Backbone Description: Backbone Marker:Genewiz; Backbone Size:2626; Vector Backbone:pUC57-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33693628
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.07.02.185249v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_158533 Copy
Species: Caenorhabditis elegans
Genetic Insert: Ptag-168::GCaMP6m::rpl-1a
Vector Backbone Description: Backbone Marker:C.I. Bargmann and S. MaCarroll; Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32574560
Proper citation: RRID:Addgene_158787 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prab-3::nls::GCaMP6m
Vector Backbone Description: Backbone Marker:C.I. Bargmann and S. MaCarroll; Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32574560
Proper citation: RRID:Addgene_158785 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pntc-1::GCaMP6m::rpl-1a
Vector Backbone Description: Backbone Marker:C.I. Bargmann and S. MaCarroll; Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32574560
Proper citation: RRID:Addgene_158786 Copy
Species: Caenorhabditis elegans
Genetic Insert: Unc104 (1-653)
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pET17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12351789
Proper citation: RRID:Addgene_14107 Copy
Species: Caenorhabditis elegans
Genetic Insert: hrp-1
Vector Backbone Description: Vector Backbone:pBlueScript; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139203 Copy
Species: Caenorhabditis elegans
Genetic Insert: hrp-1
Vector Backbone Description: Vector Backbone:pBlueScript; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139201 Copy
Species: Caenorhabditis elegans
Genetic Insert: hrp-1
Vector Backbone Description: Vector Backbone:pBlueScript; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139202 Copy
Species: Caenorhabditis elegans
Genetic Insert: hrp-1
Vector Backbone Description: Vector Backbone:pBlueScript; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139205 Copy
Species: Caenorhabditis elegans
Genetic Insert: hrp-1
Vector Backbone Description: Vector Backbone:pBlueScript; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139206 Copy
Species: Caenorhabditis elegans
Genetic Insert: hrp-1
Vector Backbone Description: Vector Backbone:pBlueScript; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139198 Copy
Species: Caenorhabditis elegans
Genetic Insert: ced-4
Vector Backbone Description: Backbone Size:3422; Vector Backbone:pSL1190; Vector Types:Bacterial Expression, Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8706125
Proper citation: RRID:Addgene_139645 Copy
Species: Caenorhabditis elegans
Genetic Insert: pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159838 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159799 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32908967
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159795 Copy
Species: Caenorhabditis elegans
Genetic Insert: [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159849 Copy
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