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Species: E. coli
Genetic Insert: dnaA
Vector Backbone Description: Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_257529 Copy
Species: E. coli
Genetic Insert: dnaG
Vector Backbone Description: Vector Backbone:pET-3d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_257530 Copy
Species: E. coli
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41535170
Proper citation: RRID:Addgene_254019 Copy
Species: E. coli
Genetic Insert: CmR(TAG)
Vector Backbone Description: Backbone Size:9200; Vector Backbone:BAC-phagemid; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42067623
Proper citation: RRID:Addgene_253999 Copy
Species: E. coli
Genetic Insert: Ec dinB (Pol IV)
Vector Backbone Description: Vector Backbone:pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_258212 Copy
Species: E. coli
Genetic Insert: Ec dinA (Pol II)
Vector Backbone Description: Vector Backbone:pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_258211 Copy
Species: E. coli
Genetic Insert: yfp
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 3 parts coding sequences in the CTK system
Proper citation: RRID:Addgene_248444 Copy
Species: E. coli
Genetic Insert: PTet
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2a parts are the first of tandem promoters in the CTK system
Proper citation: RRID:Addgene_248384 Copy
Species: E. coli
Genetic Insert: PSrpR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2a parts are the first of tandem promoters in the CTK system
Proper citation: RRID:Addgene_248386 Copy
Species: E. coli
Genetic Insert: Q1-QacR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2cd34 parts are expression parts in the CTK system. They just need promoters to be fully functional
Proper citation: RRID:Addgene_248510 Copy
Species: E. coli
Genetic Insert: B3-bm3R1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2cd34 parts are expression parts in the CTK system. They just need promoters to be fully functional
Please note: This plasmid contains a T nucleotide deletion in L3S2P55. This deletion is not known to impact plasmid function.
Proper citation: RRID:Addgene_248500 Copy
Species: E. coli
Genetic Insert: E coli csg operon
Vector Backbone Description: Vector Backbone:pTACxc; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40604283
Proper citation: RRID:Addgene_258143 Copy
Species: E. coli
Genetic Insert: phnD based Phosphonate Biosensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22019591
Comments:
The EcPhnD-cpGFP insertion variants were constructed by overlap PCR using the synthetic EcPhnD sequence and the cpGFP146 variant from GCaMP2. Detailed sequences are provided in attached sheets. The linker regions were mutated by single-stranded template mutagenesis
Proper citation: RRID:Addgene_33376 Copy
Species: E. coli
Genetic Insert: MBP based Maltose Biosensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21989929
Comments: The MBP-cpGFP insertion variant was constructed by overlap PCR using the wild-type MBP sequence from the pMalE-p4E vector (New England Biolabs) and the cpGFP146 variant from GCaMP2 into pRSET-A between BamHI and EcoRI sites. The N-terminal periplasmic leader sequence of MBP was excluded, and a BamHI site (encoding Gly-Ser) was included at the 5′-end. At the C-terminus, an additional His6Gly tag was included after the terminus of MBP (a cloning oversight). An EcoRI site was included after the stop codon.
Proper citation: RRID:Addgene_33372 Copy
Species: E. coli
Genetic Insert: EF-Sep
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6700; Vector Backbone:pMAL-c2x; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21868676
Comments: To construct pMAL-EFSep the gene encoding EF-Sep, was cloned between the NdeI and BamHI sites in the pET20b plasmid (Novaven) to
add a C-terminal His6 tag. This fusion construct was then PCR-amplified using primers adding MfeI and PstI restriction sites. The PCR product was cloned in-frame between EcoRI and PstI in pMALc2x (New England Biolabs) to add an N-terminal maltose binding protein (MBP) tag.
To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which is catalyzing the last step in serine biosynthesis, was deleted from Escherichia coli strains Top10 (Top10∆serB - Addgene #34928) and BL21 (BL21∆serB - Addgene #34929). These strains are required hosts when using this plasmid.
Proper citation: RRID:Addgene_34627 Copy
Species: e. coli
Genetic Insert: cytochrome b562
Vector Backbone Description: Vector Backbone:pUC 18; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1761034
Proper citation: RRID:Addgene_35041 Copy
Species: E. coli
Genetic Insert: CspRecT, mutL_E32K, E. coli SSB
Vector Backbone Description: Vector Backbone:pORTMAGE-Ec1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39237706
Proper citation: RRID:Addgene_227187 Copy
Species: E. coli
Genetic Insert: EcTyrRS
Vector Backbone Description: Vector Backbone:pAcBac3; Vector Types:Mammalian Expression, Baculoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36905325
Proper citation: RRID:Addgene_228788 Copy
Species: E. coli
Genetic Insert: EcTrpRS
Vector Backbone Description: Vector Backbone:pAcBac1; Vector Types:Mammalian Expression, Baculoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36905325
Proper citation: RRID:Addgene_228780 Copy
Species: E. coli
Genetic Insert: IraD (R70A)
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pSKB2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229459 Copy
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