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Species: Saccharomyces cerevisiae
Genetic Insert: tHMGR
Vector Backbone Description: Vector Backbone:pLRD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_208266 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tHMGR
Vector Backbone Description: Vector Backbone:pLRD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_208268 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tHMGR
Vector Backbone Description: Vector Backbone:pLRD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_208271 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tHMGR
Vector Backbone Description: Vector Backbone:pLRD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_208272 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mms2
Vector Backbone Description: Vector Backbone:pET30; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38103558
Comments: pHU2807
Proper citation: RRID:Addgene_212716 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ubc7
Vector Backbone Description: Vector Backbone:pMAL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38103558
Comments: pHU3365
Proper citation: RRID:Addgene_212713 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dCas9-Set2(R195G, C201A)
Vector Backbone Description: Backbone Size:8173; Vector Backbone:hSyn-GW-IRES2-mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34480866
Proper citation: RRID:Addgene_202090 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BdGAPDHpro-Hshph-G4S-nanoluc-ScADH1ter
Vector Backbone Description: Backbone Marker:Edgar Medina; Backbone Size:9378; Vector Backbone:pGI3EM17; Vector Types:Bacterial Expression, Yeast Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38236738
Comments: Please visit https://doi.org/10.1101/2023.10.17.561934 for bioRxiv preprint.
Proper citation: RRID:Addgene_210582 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BdTUBA1pro-Hshph-G4S-nanoluc-ScADH1ter
Vector Backbone Description: Backbone Marker:Edgar Medina; Backbone Size:9378; Vector Backbone:pGI3EM17; Vector Types:Bacterial Expression, Yeast Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38236738
Comments: Please visit https://doi.org/10.1101/2023.10.17.561934 for bioRxiv preprint.
Proper citation: RRID:Addgene_210586 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter
Vector Backbone Description: Backbone Marker:Edgar Medina; Backbone Size:9375; Vector Backbone:pGI3EM30; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38236738
Comments: Please visit https://doi.org/10.1101/2023.10.17.561934 for bioRxiv preprint.
Proper citation: RRID:Addgene_210598 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
Vector Backbone Description: Backbone Marker:Edgar Medina; Backbone Size:9376; Vector Backbone:pGI3EM11; Vector Types:Bacterial Expression, Yeast Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38236738
Comments: Please visit https://doi.org/10.1101/2023.10.17.561934 for bioRxiv preprint.
Proper citation: RRID:Addgene_210569 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tRNA-gRNA scaffold
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:3500; Vector Backbone:pCR-Blunt II-TOPO; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37326023
Comments: A common PCR template used fo CRISPR Engineering of EPisomes in Yeast, or CREEPY, a method for rapid engineering of large synthetic episomal DNA constructs. This is designed and assembled based on the template consisting of gRNA scaffold with a tRNA sequence, as published in Zhang, Yueping, et al. "A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae." Nature communications 10.1 (2019): 1053.
Proper citation: RRID:Addgene_200227 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cas9 (codon optimized for human)
Vector Backbone Description: Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37326023
Proper citation: RRID:Addgene_192603 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cas9 (codon optimized for human)
Vector Backbone Description: Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37326023
Proper citation: RRID:Addgene_187970 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gal4 DBD(1-147)
Vector Backbone Description: Backbone Size:2924; Vector Backbone:pECE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25963659
Proper citation: RRID:Addgene_71728 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CUS2p
Vector Backbone Description: Backbone Marker:Midwest Center for Structural Genomics; Backbone Size:5704; Vector Backbone:p11; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26631165
Proper citation: RRID:Addgene_73060 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ste2p-Citrine, Cyc1 term, Ste3p-mCherry
Vector Backbone Description: Vector Backbone:p41Neo 3-fragment GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_58564 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ste2p-Citrine, Cyc1 term, Ste3p-mCherry
Vector Backbone Description: Vector Backbone:p41Nat 3-fragment GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_58563 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 2 x Osh2 PH domain
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15271978
Comments: This plasmid contains two copies of Osh2 PH domain. Osh2 PH domain= Proline 256 - Proline 424
Proper citation: RRID:Addgene_58830 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Scs2
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12727870
Proper citation: RRID:Addgene_58834 Copy
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