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Species: Synthetic
Genetic Insert: ProC mCherry TT
Vector Backbone Description: Vector Backbone:pFPV-mCherry; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29201859
Proper citation: RRID:Addgene_112521 Copy
Species: Homo sapiens
Genetic Insert: RIPK1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_112489 Copy
Species: Synthetic
Genetic Insert: Tol2 Transposase
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27042235
Proper citation: RRID:Addgene_112486 Copy
Species: Lactococcus lactis
Genetic Insert: HrtR
Vector Backbone Description: Backbone Size:2305; Vector Backbone:pZA1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29798884
Proper citation: RRID:Addgene_112529 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Stable ACT1 intron (Mut)
Vector Backbone Description: Backbone Size:5731; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30651636
Proper citation: RRID:Addgene_127609 Copy
Genetic Insert: Gal80
Vector Backbone Description: Backbone Marker:Justin Bosch and Norbert Perrimon; Vector Backbone:pCRISPaint-T2A-Gal4-3xP3-RFP digested with NheI/KpnI; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31685521
Proper citation: RRID:Addgene_127562 Copy
Genetic Insert: FLPo
Vector Backbone Description: Backbone Marker:Justin Bosch and Norbert Perrimon; Vector Backbone:pCRISPaint-T2A-Gal4-3xP3-RFP digested with NheI/KpnI; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31685521
Proper citation: RRID:Addgene_127561 Copy
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Marker:Justin Bosch and Norbert Perrimon; Vector Backbone:pCRISPaint-T2A-Gal4-3xP3-RFP digested with NotI/KpnI; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31685521
Proper citation: RRID:Addgene_127566 Copy
Genetic Insert: p65
Vector Backbone Description: Backbone Marker:Justin Bosch and Norbert Perrimon; Vector Backbone:pCRISPaint-T2A-Gal4-3xP3-RFP digested with NheI/KpnI; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31685521
Proper citation: RRID:Addgene_127565 Copy
Genetic Insert: Gal4DBD
Vector Backbone Description: Backbone Marker:Justin Bosch and Norbert Perrimon; Vector Backbone:pCRISPaint-T2A-Gal4-3xP3-RFP digested with NheI/KpnI; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31685521
Proper citation: RRID:Addgene_127564 Copy
Genetic Insert: Nluc
Vector Backbone Description: Backbone Marker:Justin Bosch and Norbert Perrimon; Vector Backbone:pCRISPaint-T2A-Gal4-3xP3-RFP digested with NheI/KpnI; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31685521
Proper citation: RRID:Addgene_127563 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HNT2 intron
Vector Backbone Description: Backbone Size:4778; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30651636
Proper citation: RRID:Addgene_127603 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ACT1 intron
Vector Backbone Description: Backbone Size:4778; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30651636
Proper citation: RRID:Addgene_127602 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ECM33 intron with MS2 hairpins inserted near 3′ end of intron
Vector Backbone Description: Backbone Size:5876; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30651636
Proper citation: RRID:Addgene_127601 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron
Vector Backbone Description: Backbone Size:5876; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30651636
Proper citation: RRID:Addgene_127600 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL27B intron
Vector Backbone Description: Backbone Size:4778; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30651636
Proper citation: RRID:Addgene_127604 Copy
Species: Synthetic
Genetic Insert: F3-flanked spectinomycin resistance cassette
Vector Backbone Description: Backbone Marker:KEIO collection material; Backbone Size:2000; Vector Backbone:pKD13; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38488830
Comments: Please visit https://doi.org/10.1101/2024.01.17.576040 for bioRxiv preprint.
Proper citation: RRID:Addgene_127551 Copy
Species: Synthetic
Genetic Insert: F5-flanked tetracycline resistance cassette
Vector Backbone Description: Backbone Marker:KEIO collection material; Backbone Size:2000; Vector Backbone:pKD13; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_127552 Copy
Genetic Insert: QF2
Vector Backbone Description: Backbone Marker:Justin Bosch and Norbert Perrimon; Vector Backbone:pCRISPaint-T2A-Gal4-3xP3-RFP digested with NheI/KpnI; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31685521
Proper citation: RRID:Addgene_127559 Copy
Genetic Insert: Integrase 9 coding sequence codon optimized for A. thaliana expression.
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The Actin 2 promoter came from Aragao, F. J. L., et al. Plant Science 168, 1227-1233 (2005) and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). The original integrase 9 coding sequence (before A. thaliana codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).
Proper citation: RRID:Addgene_127533 Copy
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