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Genetic Insert: sgSOD1, CAG promoter, mCherry, puro
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25658371
Proper citation: RRID:Addgene_63711 Copy
Species: Ciona
Genetic Insert: CC6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25568129
Proper citation: RRID:Addgene_63718 Copy
Species: Ciona
Genetic Insert: Triple mutant
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA 3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25568129
Proper citation: RRID:Addgene_63719 Copy
Species: Synthetic
Genetic Insert: EGFP-2A-PuroR
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26678082
Proper citation: RRID:Addgene_63672 Copy
Species: Mus musculus
Genetic Insert: HDAC3
Vector Backbone Description: Backbone Size:33000; Vector Backbone:pAdeno-X; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16982804
Proper citation: RRID:Addgene_63675 Copy
Species: Streptococcus pneumoniae
Genetic Insert: acpS
Vector Backbone Description: Backbone Marker:Novagen ; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63687 Copy
Species: Mus musculus
Genetic Insert: BLBP
Vector Backbone Description: Backbone Size:1700; Vector Backbone:pCAG-mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23345401
Proper citation: RRID:Addgene_63721 Copy
Species: Staphylococcus aureus
Genetic Insert: ACP
Vector Backbone Description: Backbone Marker:Novagen ; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63686 Copy
Genetic Insert: FLIPQTV3.0 sensor, 2mM affinity
Vector Backbone Description: Backbone Size:5427; Vector Backbone:pcDNA3.1 (-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22723868
Proper citation: RRID:Addgene_63731 Copy
Species: V. cholerae
Genetic Insert: nanH
Vector Backbone Description: Backbone Marker:Franklin lab; Vector Backbone:pCVD315; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2832365
Comments: See also Galen et. al. 1990. Advances in Research on Cholera and related Diarrheas, 7, eds., R.B. Sack, Y. Zinnaka p143-153 KTK Scientific Publishers, Tokyo
Proper citation: RRID:Addgene_63739 Copy
Species: Stickleback (G. aculeatus)
Genetic Insert: 3.2 kb marine stickleback armor plate enhancer
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4890; Vector Backbone:pTA-Luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25629660
Proper citation: RRID:Addgene_63696 Copy
Species: Drosophila melanogaster
Genetic Insert: immune deficiency
Vector Backbone Description: Backbone Marker:lifetechnologies; Backbone Size:3500; Vector Backbone:pMT-V5-His; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: *Note: Addgene's quality control sequencing finds two amino acid residue substitutions, L47M and H212Y, in the Drosophila IMD insert. These residue changes are not thought to affect plasmid function, and the protein has been shown to activate the Imd pathway as expected.
Proper citation: RRID:Addgene_63748 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dCas9-VPR
Vector Backbone Description: Vector Backbone:pAG414GPD; Vector Types:Yeast Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25730490
Comments: Cas9 contains D839A and N863A mutations relative to reference sequence (WP_010922251.1). Depositor states that these mutations do not affect function.
Proper citation: RRID:Addgene_63801 Copy
Species: Drosophila melanogaster
Genetic Insert: SP-dCas9-VPR
Vector Backbone Description: Vector Backbone:pAWG; Vector Types:Insect Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25730490
Comments: Please note that this plasmid contains a stop codon between dCas9-VPR and eGFP and does NOT produce a GFP fusion protein.
Cas9 contains D839A and N863A mutations. Depositor states that these mutations should not affect function.
Proper citation: RRID:Addgene_63802 Copy
Species: Homo sapiens
Genetic Insert: dCas9-VPR
Vector Backbone Description: Vector Backbone:PB-TRE; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology, Piggybac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25730490
Proper citation: RRID:Addgene_63800 Copy
Species: Other
Genetic Insert: PhlF and LacI
Vector Backbone Description: Vector Backbone:P15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63815 Copy
Species: E. coli cloning vector
Genetic Insert: eCitrine-Strep-tag II followed by URA3 selection marker
Vector Backbone Description: Backbone Marker:Mark A. Sheff and Kurt S. Thorn; Backbone Size:4900; Vector Backbone:pKT175; Vector Types:E. coli cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24969434
Comments: The Strep-tag II sequence is adapted from the sequence of commercial Strep-tag II plasmids offered by IBA, Goettingen, Germany.
Proper citation: RRID:Addgene_63787 Copy
Species: Synthetic
Genetic Insert: eVenus-Strep-tag II followed by URA3
Vector Backbone Description: Backbone Size:4940; Vector Backbone:pGS1212; Vector Types:E. coli cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24969434
Comments: The sequence of the eVenus gene has been optimized for S. cerevisiae and generated by gene synthesis. The Strep-tag II sequence is adapted from the sequence of commercial Strep-tag II plasmids offered by IBA, Goettingen, Germany.
Proper citation: RRID:Addgene_63788 Copy
Species: Synthetic
Genetic Insert: eSapphire-Strep-tag II followed by URA3
Vector Backbone Description: Backbone Size:4940; Vector Backbone:pGS1212; Vector Types:E. coli cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24969434
Comments: The sequence of the eSapphire gene has been optimized for S. cerevisiae and generated by gene synthesis. The Strep-tag II sequence is adapted from the sequence of commercial Strep-tag II plasmids offered by IBA, Goettingen, Germany.
Proper citation: RRID:Addgene_63784 Copy
Species: Homo sapiens
Genetic Insert: SP-dCas9-VPR
Vector Backbone Description: Vector Backbone:pcDNA3.3 TOPO; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25730490
Comments: Cas9 insert contains K604R, D839A and N863A mutations relative to reference sequence WP_010922251.1. Depositor states that these mutations should not affect function.
This plasmid contains a human codon optimized form of cas9.
Proper citation: RRID:Addgene_63798 Copy
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