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Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62143 Copy
Species: Homo sapiens
Genetic Insert: WFS1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20876400
Proper citation: RRID:Addgene_62051 Copy
Species: Homo sapiens
Genetic Insert: MDM2
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4985; Vector Backbone:PGEX-6P-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25362358
Comments: Prescission cleavage.
Proper citation: RRID:Addgene_62063 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62113 Copy
Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:26577727
Comments: Genotype = proC
Precursor strain = BL21 CodonPlus (DE3)-RIL
Selective amino acid labeling (and/or requirement) = Pro
Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system.
Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain.
This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant.
Proper citation: RRID:Addgene_62074 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62128 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET28A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15824059
Comments: Includes a thrombin cleavage site
Proper citation: RRID:Addgene_62082 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62084 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62090 Copy
Species: Synthetic
Genetic Insert: human codon optimized Cas9
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62134 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62131 Copy
Species: S. Pyogenes
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pNH605; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62277 Copy
Species: Synthetic
Genetic Insert: iRFP
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62157 Copy
Genetic Insert: cas9
Vector Backbone Description: Backbone Marker:Johannes Bischof and Konrad Basler; Backbone Size:9200; Vector Backbone:pnos; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25002478
Comments: Please visit crisprflydesign.org for more information.
Please acknowledge Fillip Port and Simon Bullock when publishing work derived from the use of this plasmid.
Proper citation: RRID:Addgene_62208 Copy
Species: Synthetic
Genetic Insert: gRNA scaffold
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pCambia; Vector Types:Plant Expression, plant binary vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_62202 Copy
Species: Synthetic
Genetic Insert: gRNA scaffold
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pCambia; Vector Types:Plant Expression, plant binary vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_62203 Copy
Genetic Insert: PCP-VP64
Vector Backbone Description: Vector Backbone:pNH603; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62280 Copy
Genetic Insert: COM-VP64
Vector Backbone Description: Vector Backbone:pNH603; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62281 Copy
Species: Synthetic
Genetic Insert: gRNA scaffold
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pCambia; Vector Types:Plant Expression, plant binary vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_62201 Copy
Genetic Insert: MCP-VP64
Vector Backbone Description: Vector Backbone:pNH605; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62282 Copy
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