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Vector Backbone Description: Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:13668; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The insert begins with a Gateway attR destination cloning cassette (for insertion of gene regulatory sequences), followed by a DSCP synthetic core promoter, a Syn21 translation enhancer, a QF:GAL4 hybrid transcription factor (QF DNA binding domain, Gal4 activation domain) followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint.
Proper citation: RRID:Addgene_173729 Copy
Genetic Insert: epegRNA
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40555761
Comments: Please visit https://doi.org/10.1101/2024.06.16.599253 for the bioRxiv preprint.
Proper citation: RRID:Addgene_240539 Copy
Species: Synthetic
Genetic Insert: attR1-CmR-ccdB-attR2
Vector Backbone Description: Vector Backbone:pCAGEN-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_242575 Copy
Vector Backbone Description: Vector Backbone:pCAGEN-DEST; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_242573 Copy
Vector Backbone Description: Vector Backbone:pCAGEN-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_242577 Copy
Vector Backbone Description: Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:11967; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The insert begins with a Gateway attR destination cloning cassette (for insertion of gene regulatory sequences), followed by a DSCP synthetic core promoter, a Syn21 translation enhancer, a QF2 transcription factor followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint.
Proper citation: RRID:Addgene_173728 Copy
Vector Backbone Description: Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:11484; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The insert begins with 20 copies of the UAS binding sequence followed by a DSCP synthetic core promoter, a Gateway attR destination cloning cassette followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint.
Proper citation: RRID:Addgene_173723 Copy
Vector Backbone Description: Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:11397; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The insert begins with 13 copies of the LexA binding sequence followed by a DSCP synthetic core promoter, a Gateway attR destination cloning cassette followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint.
Proper citation: RRID:Addgene_173724 Copy
Vector Backbone Description: Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:11189; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The insert begins with 10 copies of the UAS binding sequence followed by a DSCP synthetic core promoter, a Gateway attR destination cloning cassette followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint.
Proper citation: RRID:Addgene_173725 Copy
Vector Backbone Description: Backbone Marker:Evelyne Ruchti and Brian McCabe; Backbone Size:12063; Vector Backbone:pBID2; Vector Types:Insect Expression, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The insert begins with a Gateway attR destination cloning cassette (for insertion of gene regulatory sequences), followed by a DSCP synthetic core promoter, a Syn21 translation enhancer, a LexA:QF hybrid transcription factor (LexA DNA binding domain, QF activation domain) followed by a p10 3' untranslated region. The insert is flanked by Gypsy insulator sequences to enable more homogeneous transgene expression at diverse genetic insertion sites. The backbone includes a phiC31 attB sequence for targeted transgenesis and a mini-white gene to allow transgene selection. Please visit https://doi.org/10.64898/2026.02.02.703225 for bioRxiv preprint.
Proper citation: RRID:Addgene_173727 Copy
Vector Backbone Description: Vector Backbone:Other; Vector Types:subcloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_231170 Copy
Vector Backbone Description: Vector Backbone:Other; Vector Types:subcloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_231172 Copy
Vector Backbone Description: Backbone Marker:David Root (Addgene #41392); Vector Backbone:pLEX307; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:42156379
Proper citation: RRID:Addgene_247684 Copy
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:42056505
Comments: Please visit https://doi.org/10.1101/2025.07.23.666448 for bioRxiv preprint.
Proper citation: RRID:Addgene_253774 Copy
Species: Synthetic
Genetic Insert: TRE: iCre expression cassette
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pTolDEST_R4R3 (Gateway); Vector Types:Cre/Lox, Gateway Cloning, vertebrate expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_257881 Copy
Vector Backbone Description: Backbone Size:7522; Vector Backbone:pKW32; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pWK32 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing a hemoglobin tag.
Proper citation: RRID:Addgene_18669 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7694; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pcDNA3 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a mammalian expression clone.
Proper citation: RRID:Addgene_18672 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:11282; Vector Backbone:pPIC3.5K; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pPIC3.5K Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction for integration of gene of interest into Pichia.
Proper citation: RRID:Addgene_18707 Copy
Vector Backbone Description: Backbone Size:9313; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_19065 Copy
Vector Backbone Description: Backbone Size:9778; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_19067 Copy
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