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Species: Synthetic
Genetic Insert: RaTG13 N
Vector Backbone Description: Vector Backbone:pCG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33974846
Proper citation: RRID:Addgene_179987 Copy
Species: Synthetic
Genetic Insert: SARS-CoV-2 Nsp7
Vector Backbone Description: Vector Backbone:pCG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33974846
Proper citation: RRID:Addgene_179974 Copy
Species: Synthetic
Genetic Insert: SARS-CoV-1 ORF6
Vector Backbone Description: Vector Backbone:pCG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33974846
Proper citation: RRID:Addgene_179970 Copy
Species: Synthetic
Genetic Insert: CMV promoter sgRNA
Vector Backbone Description: Backbone Marker:Dr. Feng Zhang Lab; Vector Backbone:pX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35789697
Comments: This plasmid was cloned with the one step BbsI restriction enzyme cloning method. Please note that gRNA sequences that do not start with a G, need a G added to the sequence before cloning for U6 promoter transcription.
Proper citation: RRID:Addgene_179915 Copy
Species: Synthetic
Genetic Insert: mGreenLantern sgRNA
Vector Backbone Description: Backbone Marker:Dr. Feng Zhang Lab; Vector Backbone:pX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35789697
Comments: This plasmid was cloned with the one step BbsI restriction enzyme cloning method. Please note that gRNA sequences that do not start with a G, need a G added to the sequence before cloning for U6 promoter transcription.
Proper citation: RRID:Addgene_179914 Copy
Species: Synthetic
Genetic Insert: tdTomato sgRNA
Vector Backbone Description: Backbone Marker:Dr. Feng Zhang Lab; Vector Backbone:pX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35789697
Comments: This plasmid was cloned with the one step BbsI restriction enzyme cloning method. Please note that gRNA sequences that do not start with a G, need a G added to the sequence before cloning for U6 promoter transcription.
Proper citation: RRID:Addgene_179919 Copy
Species: Synthetic
Genetic Insert: CMV promoter sgRNA
Vector Backbone Description: Backbone Marker:Dr. Feng Zhang Lab; Vector Backbone:pX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35789697
Comments: This plasmid was cloned with the one step BbsI restriction enzyme cloning method. Please note that gRNA sequences that do not start with a G, need a G added to the sequence before cloning for U6 promoter transcription.
Proper citation: RRID:Addgene_179917 Copy
Species: Synthetic
Genetic Insert: GBP nanobody
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pUASp-attB-K10; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35020877
Proper citation: RRID:Addgene_179995 Copy
Species: Synthetic
Genetic Insert: GBP nanobody
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pUASt-attB-K10; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35020877
Proper citation: RRID:Addgene_179996 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:CMVTO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35177637
Proper citation: RRID:Addgene_179912 Copy
Species: Synthetic
Genetic Insert: TadA8e-dSiCas12i(D1049A)
Vector Backbone Description: Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37378659
Proper citation: RRID:Addgene_179992 Copy
Species: Synthetic
Genetic Insert: Kir2.1
Vector Backbone Description: Vector Backbone:CMVTO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35177637
Proper citation: RRID:Addgene_179707 Copy
Species: Synthetic
Genetic Insert: TrpLE-M-nMSP1E3D1
Vector Backbone Description: Backbone Marker:Gene Universal; Backbone Size:5310; Vector Backbone:pET-24a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34848201
Proper citation: RRID:Addgene_179949 Copy
Species: Synthetic
Genetic Insert: Kir2.1
Vector Backbone Description: Vector Backbone:CMVTO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35177637
Proper citation: RRID:Addgene_179705 Copy
Species: Synthetic
Genetic Insert: PEmax-P2A-EGFP
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34653350
Proper citation: RRID:Addgene_180020 Copy
Species: Synthetic
Genetic Insert: Gpb6 nanobody
Vector Backbone Description: Vector Backbone:CMVTO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35177637
Proper citation: RRID:Addgene_180022 Copy
Species: Synthetic
Genetic Insert: EGFR sgRNA
Vector Backbone Description: Vector Backbone:pXPR_502; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35113687
Proper citation: RRID:Addgene_180267 Copy
Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Bacterial Expression, Mouse Targeting, dre/rox; Bacterial Resistance:Ampicillin
Comments: A reporter expression cassette with short homology arms to Rosa26 on either side. Constructed originally for generating Rosa26 reporter allele targeting vector by recombineering. Untested for other purposes, but may be useful for CRISPR knock-in as well.
Proper citation: RRID:Addgene_180154 Copy
Species: Synthetic
Genetic Insert: td-sfGFP
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32183906
Comments: Similar to the situation with some other plasmids in this collection, the missing four nucleotides at the 5' end of the WPRE renders it unrecognizable to SnapGene, but the expression vector is functional.
Proper citation: RRID:Addgene_180150 Copy
Species: Synthetic
Genetic Insert: td-sfGFP
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Bacterial Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: A reporter expression cassette with short homology arms to Hipp11 on either side. Constructed originally for generating Hipp11 reporter allele targeting vector by recombineering. Untested for other purposes, but may be useful for CRISPR knock-in as well.
Proper citation: RRID:Addgene_180155 Copy
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