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Species: Rattus norvegicus
Genetic Insert: G-alpha-s-EE-mCerulean
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15297467
Comments: Reference for alpha-s-EE: Wilson, P. T., and Bourne, H. R. (1995) Fatty acylation of αz. Effects of palmitoylation and myristoylation on αz signaling. J. Biol. Chem. 270, 9667-9675
Reference for mCerulean: Rizzo MA, Springer GH, Granada B, Piston DW. An improved cyan fluorescent protein variant useful for FRET. Nat Biotechnol. 2004;22(4):445-9. PubMed PMID: 14990965.
Proper citation: RRID:Addgene_66084 Copy
Species: Rattus norvegicus
Genetic Insert: G-alpha-s-EE-EGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15297467
Comments: Reference for alpha-s-EE: Wilson, P. T., and Bourne, H. R. (1995) Fatty acylation of αz. Effects of palmitoylation and myristoylation on αz signaling. J. Biol. Chem. 270, 9667-9675
Proper citation: RRID:Addgene_66083 Copy
Species: Rattus norvegicus
Genetic Insert: Opto Mu opioid receptor
Vector Backbone Description: Backbone Size:6330; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25937173
Proper citation: RRID:Addgene_66847 Copy
Species: Rattus norvegicus
Genetic Insert: ErbB2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16352662
Proper citation: RRID:Addgene_66945 Copy
Species: Rattus norvegicus
Genetic Insert: ErbB2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1D/V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17626164
Comments: A24T is a genetic variation and has no effect on function.
Proper citation: RRID:Addgene_66946 Copy
Species: Rattus norvegicus
Genetic Insert: NCX3.1
Vector Backbone Description: Vector Backbone:pBS?; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8798769
Proper citation: RRID:Addgene_67147 Copy
Species: Rattus norvegicus
Genetic Insert: OCT-HA-eGFP
Vector Backbone Description: Vector Backbone:pCMV(0); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27057421
Comments: Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the matrix using the leader sequence from ornithine carbamylase transferase (OCT): Horwich, et al (1985) EMBO J (PMCID: 554314); and (3) Harder, et al (2004) Current Biology 14:340-348
Proper citation: RRID:Addgene_67479 Copy
Species: Rattus norvegicus
Genetic Insert: OCT-HA-eGFP
Vector Backbone Description: Vector Backbone:pCMV(delta4); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27057421
Comments: Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the matrix using the leader sequence from ornithine carbamylase transferase (OCT): Horwich, et al (1985) EMBO J (PMCID: 554314); and (3) Harder, et al (2004) Current Biology 14:340-348
Proper citation: RRID:Addgene_67483 Copy
Species: Rattus norvegicus
Genetic Insert: OCT-HA-eGFP
Vector Backbone Description: Vector Backbone:pCMV(delta2); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27057421
Comments: Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the matrix using the leader sequence from ornithine carbamylase transferase (OCT): Horwich, et al (1985) EMBO J (PMCID: 554314); and (3) Harder, et al (2004) Current Biology 14:340-348
Proper citation: RRID:Addgene_67481 Copy
Species: Rattus norvegicus
Genetic Insert: OCT-HA-eGFP
Vector Backbone Description: Vector Backbone:pCMV(delta3); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27057421
Comments: Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the matrix using the leader sequence from ornithine carbamylase transferase (OCT): Horwich, et al (1985) EMBO J (PMCID: 554314); and (3) Harder, et al (2004) Current Biology 14:340-348
Proper citation: RRID:Addgene_67482 Copy
Species: Rattus norvegicus
Genetic Insert: mRuby2-P2A-GCaMP6f
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27284193
Comments: The discrepancies between the full sequence and Addgene's QC sequence should not have any functional consequence.
mRuby2:
Article: Improving FRET dynamic range with bright green and red fluorescent proteins. Lam et al (Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171. PubMed)
Addgene Plasmid 40260
GCaMP6f:
Article: Ultrasensitive fluorescent proteins for imaging neuronal activity. Chen et al (Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354. PubMed)
Addgene Plasmid 40755
Proper citation: RRID:Addgene_68719 Copy
Species: Rattus norvegicus
Genetic Insert: Gephyrin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pCR3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23408424
Proper citation: RRID:Addgene_68818 Copy
Species: Rattus norvegicus
Genetic Insert: Gephyrin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pCR3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23408424
Proper citation: RRID:Addgene_68817 Copy
Species: Rattus norvegicus
Genetic Insert: Collybistin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29069083
Comments: CB2SH3+ contains the SH3 domain splice cassette, CB2SH3- lacks the Sh3 domain splice cassette. That is the only difference between these two splice isoforms.
Proper citation: RRID:Addgene_68822 Copy
Species: Rattus norvegicus
Genetic Insert: Cx43
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26265468
Comments: Note all connexins tested that have a fluorescent protein fused to the connexin amino-terminus do not form functional channels. This plasmid forms gap junction plaque structures but does not produce intercellular coupling.
Proper citation: RRID:Addgene_69024 Copy
Species: Rattus norvegicus
Genetic Insert: Gja2
Vector Backbone Description: Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26265468
Comments: Note all connexins tested that have a fluorescent protein fused to the connexin amino-terminus do not form functional channels. This plasmid forms gap junction plaque structures but does not produce intercellular coupling.
Proper citation: RRID:Addgene_69021 Copy
Species: Rattus norvegicus
Genetic Insert: Cx26
Vector Backbone Description: Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26265468
Proper citation: RRID:Addgene_69026 Copy
Species: Rattus norvegicus
Genetic Insert: BE2
Vector Backbone Description: Backbone Marker:origene; Backbone Size:3399; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27096365
Proper citation: RRID:Addgene_73020 Copy
Species: Rattus norvegicus
Genetic Insert: BE1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5334; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27096365
Proper citation: RRID:Addgene_73018 Copy
Species: Rattus norvegicus
Genetic Insert: Psd95 shRNA
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16815335
Comments: Map: FHUGW-(pUb)-HinDIII-PstI-SalI-XbaI-XbaI-(p95)-KpnI-ApaI-SmaI-BamHI-(EGFP)-BglII-BsrBI-EcoRV-HinDIII-ClaI
Also associated with Xu, et al. Neuron. 2008 Jan 24;57(2):248-62. doi: 10.1016 (PMID 18215622).
Proper citation: RRID:Addgene_74013 Copy
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