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Vector Backbone Description: Backbone Size:11563; Vector Backbone:pNOC-ARS-CRISPR-35S; Vector Types:Algae, Nannochloropsis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29518315
Proper citation: RRID:Addgene_101009 Copy
Species: Homo sapiens
Genetic Insert: histone cluster 1 H2B family member j
Vector Backbone Description: Backbone Size:5858; Vector Backbone:pSNAP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Originally cloned at New England Biolabs. The H2B insert is a N-terminal insert.
This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at [email protected]. For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/
Proper citation: RRID:Addgene_101124 Copy
Vector Backbone Description: Backbone Size:5543; Vector Backbone:pACP-tag (m)- 2 Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Originally cloned at NEB. The ACP-tag is N-terminal in the vector.
For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/
This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
Proper citation: RRID:Addgene_101126 Copy
Species: Synthetic
Genetic Insert: chimeric fungal ITS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2374; Vector Backbone:pMA-T; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101174 Copy
Species: Synthetic
Genetic Insert: V4 16S rRNA chimeric spike
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2374; Vector Backbone:pMA-T; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101172 Copy
Species: Synthetic
Genetic Insert: chimeric 18S rRNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2380; Vector Backbone:pMA-T; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101173 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9 variant K848A/K1003A/R1060A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101215 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101217 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9 variant N692A/M694A/Q695A/H698A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101218 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cry2PHR
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28041848
Comments: Our Cry2WT contains an additional 29 amino acids at the C-terminus of Cry2PHR. These additional residues are also retained in other constructs we used in the associated study.
Proper citation: RRID:Addgene_101221 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cry2olig
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28041848
Comments: Our Cry2WT contains an additional 29 amino acids at the C-terminus of Cry2PHR. These additional residues are also retained in other constructs we used in the associated study.
Proper citation: RRID:Addgene_101222 Copy
Species: Homo sapiens
Genetic Insert: FUS(aa1-214)
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28041848
Comments: Our Cry2WT contains an additional 29 amino acids at the C-terminus of Cry2PHR. These additional residues are also retained in other constructs we used in the associated study.
Proper citation: RRID:Addgene_101224 Copy
Species: Homo sapiens
Genetic Insert: DDX4(1-236)
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28041848
Comments: Our Cry2WT contains an additional 29 amino acids at the C-terminus of Cry2PHR. These additional residues are also retained in other constructs we used in the associated study.
Proper citation: RRID:Addgene_101225 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9 variant N497A/R661A/Q695A/Q926A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101209 Copy
Species: Other
Genetic Insert: MCP-YFP
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28290446
Proper citation: RRID:Addgene_101160 Copy
Species: Other
Genetic Insert: YPet(short)-HP35st-mCherry
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6238; Vector Backbone:pLPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26523364
Proper citation: RRID:Addgene_101251 Copy
Species: Homo sapiens
Genetic Insert: kappa-HA-dL5-myc-ADRB2
Vector Backbone Description: Backbone Size:6437; Vector Backbone:pBABE-SacLac; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29283245
Comments: Originally used in publication:
John Holleran, Dara Brown,* Margaret H. Fuhrman, Sally A. Adler, Gregory W. Fisher, and Jonathan W. Jarvik
Fluorogen-Activating Proteins as Biosensors of Cell-Surface Proteins in Living Cells
Cytometry A. 2010 Aug; 77(8): 776–782.
Proper citation: RRID:Addgene_101253 Copy
Species: Other
Genetic Insert: YPet(short)-HP35-mCherry
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6238; Vector Backbone:pLPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26523364
Proper citation: RRID:Addgene_101250 Copy
Vector Backbone Description: Backbone Size:5802; Vector Backbone:pSNAP-tag (m) Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Codon usage of the gene is optimized for expression in mammalian cells. pSNAP-tag(m) contains two multiple cloning sites to allow cloning of the fusion partner as a fusion to the N- or C-terminus of the SNAP-tag. to allow cloning of the fusion partner as a fusion to the N- or C-terminus of the SNAP-tag. For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/
This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
Proper citation: RRID:Addgene_101135 Copy
Vector Backbone Description: Backbone Size:3725; Vector Backbone:pSNAP-tag (T7) Vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: pSNAP-tag(T7) is a high copy number E. coli expression plasmid encoding SNAP26b, a SNAP-tag protein, which can be expressed under the control of the T7 promoter. Codon usage of the gene is optimized for expression in E. coli. The fusion partner should be cloned as a fusion to the C-terminus of the SNAP-tag. For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/
This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
Proper citation: RRID:Addgene_101137 Copy
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