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Species: Homo sapiens
Genetic Insert: ST1-dCas9-VPR
Vector Backbone Description: Vector Backbone:pcDNA3.3 TOPO; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25730490
Comments: Cas9 insert contains K604R, D839A and N863A mutations relative to reference sequence WP_010922251.1. Depositor states that these mutations should not affect function.
Proper citation: RRID:Addgene_63799 Copy
Species: Other
Genetic Insert: rpoS, mRFP1 and GFPmut3b
Vector Backbone Description: Vector Backbone:P15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63839 Copy
Species: Synthetic
Genetic Insert: CBP-3xFLAG-TEV-Protein A followed by KlLEU2
Vector Backbone Description: Backbone Marker:Fermentas/Thermo ; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:E. coli cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25486077
Proper citation: RRID:Addgene_63796 Copy
Species: Synthetic
Genetic Insert: eGFP-Twin-Strep tag followed by URA3
Vector Backbone Description: Backbone Size:4940; Vector Backbone:pGS1212; Vector Types:E. coli cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24969434
Comments: Derived from plasmid pGS1212 by site-directed mutagenesis. The Twin-Strep tag sequence is adapted from the sequence of commercial Twin-Strep tag plasmids offered by IBA, Goettingen, Germany.
Proper citation: RRID:Addgene_63791 Copy
Species: Other
Genetic Insert: yqhD, mRFP1 and GFPmut3b
Vector Backbone Description: Vector Backbone:P15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63845 Copy
Species: Other
Genetic Insert: dps, mRFP1 and GFPmut3b
Vector Backbone Description: Vector Backbone:P15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63843 Copy
Species: Other
Genetic Insert: katG, mRFP1 and GFPmut3b
Vector Backbone Description: Vector Backbone:P15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63844 Copy
Species: Other
Genetic Insert: rpoS, mRFP1 and GFPmut3b
Vector Backbone Description: Vector Backbone:P15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63840 Copy
Species: Synthetic
Genetic Insert: No-aptamer control mRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26621913
Proper citation: RRID:Addgene_63856 Copy
Species: Synthetic
Genetic Insert: TMR7 TMR riboswitch
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26621913
Proper citation: RRID:Addgene_63857 Copy
Species: Synthetic
Genetic Insert: TMR10 TMR riboswitch
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26621913
Proper citation: RRID:Addgene_63858 Copy
Vector Backbone Description: Backbone Size:5693; Vector Backbone:pYC12; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25986172
Proper citation: RRID:Addgene_63913 Copy
Vector Backbone Description: Backbone Size:5693; Vector Backbone:pYC12; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25986172
Proper citation: RRID:Addgene_63914 Copy
Vector Backbone Description: Backbone Marker:Zordan, et al, 2013; Backbone Size:4910; Vector Backbone:pGRB2.0; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25986172
Proper citation: RRID:Addgene_63919 Copy
Vector Backbone Description: Backbone Size:5693; Vector Backbone:pYC12; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25986172
Proper citation: RRID:Addgene_63917 Copy
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression, phage display; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1896445
Comments: pComb8 is nearly identical to the original pComb3 vector but contains the phage pVIII fusion protein for multi-valent expression along the sides of the phage via fusion with pVIII. It was designed for phage display of Fabs which are cloned into two separate cloning cassettes one chain at a time using SacI/XbaI restriction sites (for the light chain) and XhoI/SpeI restriction sites (for the heavy chain). The heavy chain is expressed fused to the pVIII. Both cassettes have the pelB leader sequence, the light chain and heavy chain-geneVIII fusion product assemble in the periplasm to form the Fab fragment. Soluble Fab can be expressed by removing the gene for the pVIII phage fusion protein by SpeI/NheI digest. Sufficient soluble protein is also found in the periplasmic space as a result of proteolysis.
Proper citation: RRID:Addgene_63889 Copy
Species: Synthetic
Genetic Insert: ER-localized low-affinity GCaMP3(10.19)
Vector Backbone Description: Backbone Marker:OTTC/NIDA; Vector Backbone:pOTTC374 - pAAV EF1a DIO iRFP; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26451944
Proper citation: RRID:Addgene_63885 Copy
Vector Backbone Description: Backbone Size:4509; Vector Backbone:pMJ22; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25986172
Proper citation: RRID:Addgene_63920 Copy
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression, phage display; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1896445
Comments: The original pComb3 vector was designed for phage display of Fabs which are cloned into two separate cloning cassettes one chain at a time using SacI/XbaI restriction sites (for the light chain) and XhoI/SpeI restriction sites (for the heavy chain). The heavy chain is expressed fused to the pVIII. Both cassettes have the pelB leader sequence, the light chain and heavy chain-geneIII fusion product assemble in the periplasm to form the Fab fragment. This vector system has also been used for the display of a wide variety of other proteins like zinc fingers, peptides, and cDNA fragments. Phage can be produced which express Fab or other proteins or peptides of interest fused to the phage pIII protein for expression on the head of the phage. Soluble Fab can be expressed by removing the gene for the pIII phage fusion protein by SpeI/NheI digest. Sufficient soluble protein is also found in the periplasmic space as a result of proteolysis.
Proper citation: RRID:Addgene_63888 Copy
Vector Backbone Description: Backbone Size:4509; Vector Backbone:pMJ22; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25986172
Proper citation: RRID:Addgene_63928 Copy
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