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Species: Other
Genetic Insert: CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36088516
Comments: L2 4X opattB middle iridiod cytosol v3: P1_4X opattB-TMV-CrISY-35Sterm + P2_4X opattB-TMV-NmMLP-masterm + P3_4X opattB-TMV-Cr7DLGT-nosterm + P4_4X opattB-TMV-Cr8HGO/GOR-g7term + P5_4X opattB-TMV-CrIO-35Sterm + P6_4X opattB-TMV-CrG8H-ocsterm
Proper citation: RRID:Addgene_177090 Copy
Species: Other
Genetic Insert: P19, CrDXS2, PaGPPS1, and CrGES
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36088516
Comments: L2 P19 + 4X opattB early iridiod chloroplast v6: P1_nosP-TMV-P19-nosterm + P2_4X opattB-TMV-cTP_CrDXS2-masterm + P3_4X opattB-TMV-cTP_PaGPPS1-ocsterm + P4_4X opattB-TMV-cTP_CrGES-g7term)
Proper citation: RRID:Addgene_177088 Copy
Genetic Insert: MG1655 PlacZYA::Plac-gfp-muNS-kan
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24361104
Comments: GFP-muNS expression is under the control of Plac promoter integrated into the endogenous lac locus. The native lac operon (lacZYA) is deleted. Kanamycin resistance casette is inserted downstream of the gfp-muNS gene for cloning purposes.
Cloning method: Lambda RED recombination. The gfp-μNS fragment was amplified from plasmid pER12 (pBAD322A-gfp-μNS), which encodes an N-terminal GFP fusion to residues 471-721 of μNS (Broering et al., 2005) and then digested by KpnI/XhoI. The nptI gene was amplified from pKD4 plasmid and digested by XhoI/SmaI. Then, gfp-μNS and nptI gene fragments were triple ligated into the pKS plasmid. The resulting plasmid was used as a PCR template to generate a gfp-μNS-nptI fragment with flanking regions complementary to lacI and cynX, respectively. This fragment was then used to replace the lac operon (lacZYA) by one-step λ-recombination (Datsenko and Wanner, 2000). The resulting gfp-μNS-nptI replacement of lacZYA was transduced back into MG1655 by P1-phage to generate strain CJW4617. See Parry et al., 2014 for more details. This strain doesn't contain any plasmid.
Proper citation: RRID:Addgene_177118 Copy
Species: Bacillus circulans
Genetic Insert: Chitinase A1 Chitin Binding Domain
Vector Backbone Description: Backbone Size:5365; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.29.437438v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_169211 Copy
Genetic Insert: pLeft
Vector Backbone Description: Vector Backbone:pMV306; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33362741
Proper citation: RRID:Addgene_169409 Copy
Genetic Insert: pLeft
Vector Backbone Description: Vector Backbone:pMV306; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33362741
Proper citation: RRID:Addgene_169411 Copy
Genetic Insert: mWasabi
Vector Backbone Description: Vector Backbone:pMV306; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33362741
Proper citation: RRID:Addgene_169412 Copy
Genetic Insert: pLeft
Vector Backbone Description: Vector Backbone:pMV306; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33362741
Proper citation: RRID:Addgene_169410 Copy
Species: Mus musculus
Genetic Insert: HaloTAG mGFAP (R236H)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_169475 Copy
Vector Backbone Description: Backbone Size:6472; Vector Backbone:pPS04; Vector Types:Allelic exchange vector; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_169472 Copy
Species: Synthetic
Genetic Insert: anti-caffeine nanobody
Vector Backbone Description: Backbone Size:4900; Vector Backbone:mEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33552855
Proper citation: RRID:Addgene_169685 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7027; Vector Backbone:Modified pGBKT7; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_169710 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MatA
Vector Backbone Description: Vector Backbone:pCR™-Blunt II-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29150593
Proper citation: RRID:Addgene_169457 Copy
Species: Synthetic
Genetic Insert: sgRNA towards glpR in Pseudomonas putida
Vector Backbone Description: Vector Backbone:pBBR1k-GFP; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34175462
Proper citation: RRID:Addgene_169873 Copy
Vector Backbone Description: Backbone Marker:BIOFACT; Vector Backbone:All in One™ vector; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32190101
Proper citation: RRID:Addgene_169786 Copy
Vector Backbone Description: Backbone Marker:BIOFACT; Vector Backbone:All in One™ vector; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32190101
Proper citation: RRID:Addgene_169781 Copy
Vector Backbone Description: Backbone Marker:BIOFACT; Vector Backbone:All in One™ vector; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32190101
Proper citation: RRID:Addgene_169782 Copy
Vector Backbone Description: Backbone Marker:BIOFACT; Vector Backbone:All in One™ vector; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32190101
Proper citation: RRID:Addgene_169780 Copy
Vector Backbone Description: Backbone Marker:BIOFACT; Vector Backbone:All in One™ vector; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32190101
Proper citation: RRID:Addgene_169779 Copy
Species: Homo sapiens
Genetic Insert: CARD8
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34019797
Proper citation: RRID:Addgene_169965 Copy
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