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Species: Synthetic
Genetic Insert: epNTR
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24848468
Proper citation: RRID:Addgene_62213 Copy
Genetic Insert: Fok1-dCas9
Vector Backbone Description: Backbone Marker:gift from Silvia Aldaz; Backbone Size:12429; Vector Backbone:pact; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Please visit crisprflydesign.org for more information.
Please acknowledge Fillip Port and Simon Bullock when publishing work derived from the use of this plasmid.
Proper citation: RRID:Addgene_62211 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Luciferase, MuLE Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62179 Copy
Species: Synthetic
Genetic Insert: sgRNA
Vector Backbone Description: Backbone Size:2000; Vector Backbone:Trc99a; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25636838
Proper citation: RRID:Addgene_62226 Copy
Species: Synthetic
Genetic Insert: ER-mRFP
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4000; Vector Backbone:clontech C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16617114
Comments: Red localization marker for mammalian endoplasmic reticulum.
Proper citation: RRID:Addgene_62236 Copy
Species: Synthetic
Genetic Insert: 3xFLAG-dCas9
Vector Backbone Description: Backbone Size:5385; Vector Backbone:p414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36325175
Comments: This plasmid is compatible with gRNA expression plasmids for budding yeast, such as Addgene Plasmid #43803 ( http://www.addgene.org/43803 ).
Proper citation: RRID:Addgene_62190 Copy
Species: Synthetic
Genetic Insert: hamster BiP-mGFP-KDEL
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4000; Vector Backbone:clontech N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20410136
Comments: Avoid overexpression. Low expressing cells are best for imaging. High expression leads to protein aggregation in the nuclear envelope. See supplementary data for original paper.
Do NOT use this as an ER marker. BiP is a functional protein and significantly impacts the ER environment increasing stress resistance. Use ER-GFP if you simply want a marker for the ER when imaging cells.
Proper citation: RRID:Addgene_62231 Copy
Species: Synthetic
Genetic Insert: hamster BiP-mCherry-KDEL
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4000; Vector Backbone:clontech N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20410136
Comments: Avoid overexpression. Low expressing cells are best for imaging. High expression leads to protein aggregation in the nuclear envelope. See supplementary data for original paper.
Do NOT use this as an ER marker. BiP is a functional protein and significantly impacts the ER environment increasing stress resistance. Use ER-mCherry if you simply want a marker for the ER when imaging cells.
Proper citation: RRID:Addgene_62233 Copy
Species: Synthetic
Genetic Insert: Left CLYBL TALEN
Vector Backbone Description: Backbone Size:4681; Vector Backbone:pZT; Vector Types:TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25587899
Comments: pZT-C13/CLYBL TALENs were assembled using RVD monomers from Golden Gate TALEN kit 1.0 (TALEN Kit #1000000016) and a mammalian TALEN expression vector pZT
Proper citation: RRID:Addgene_62196 Copy
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium; Backbone Size:6767; Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin
Comments: The pFBOH-MHL vector is a derivative of the pFBOH-LIC vector (SGC). It is a donor vector for generation of recombinant baculovirus by site-specific transposition in an E. coli host. This vector adds an 18 amino acid N-terminal fusion tag containing a 6X His followed by a TEV cleavage site. Two stop codons are included in the vector at the C-terminal cloning site.
Insertion of a DNA sequence into the cloning/expression region is performed using Clontech’s In-fusion enzyme mediated directional recombination between complementary 15 nucleotide DNA sequences at the ends of the insert (PCR product) and BseRI linearized vector. Insertion of a target sequence involves replacement of a SacB gene stuffer sequence, which provides for negative selection of the original plasmid on 5% sucrose.
Proper citation: RRID:Addgene_62304 Copy
Species: Synthetic
Genetic Insert: su9-tagBFP
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378625
Proper citation: RRID:Addgene_62383 Copy
Genetic Insert: sgRNA + 2x PP7 RNA binding module
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62317 Copy
Genetic Insert: sgRNA + 1x com RNA binding module
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62318 Copy
Genetic Insert: sgRNA + 1x MS2 binding module
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62313 Copy
Genetic Insert: sgRNA + 2x MS2 binding module
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62314 Copy
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium; Backbone Size:8362; Vector Backbone:pACYC-LIC+; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_62329 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5144; Vector Backbone:pDONR P4r-P3r; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25869647
Proper citation: RRID:Addgene_62289 Copy
Genetic Insert: sgRNA
Vector Backbone Description: Vector Backbone:MP177_U6 (derived from pSico); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62338 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5147; Vector Backbone:pDONR P4r-P3r; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25869647
Proper citation: RRID:Addgene_62291 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5103; Vector Backbone:pDONR P4r-P3r; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25869647
Proper citation: RRID:Addgene_62292 Copy
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