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Species: Caenorhabditis elegans
Genetic Insert: DAF-16a
Vector Backbone Description: Backbone Size:6288; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24671950
Proper citation: RRID:Addgene_175285 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16 d/f
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:5990; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24671950
Proper citation: RRID:Addgene_175286 Copy
Species: Caenorhabditis elegans
Genetic Insert: fbf-2 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21390 Copy
Species: Caenorhabditis elegans
Genetic Insert: pal-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21391 Copy
Species: Caenorhabditis elegans
Genetic Insert: mCherry in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21376 Copy
Species: Caenorhabditis elegans
Genetic Insert: spe-11 promoter in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21379 Copy
Species: Caenorhabditis elegans
Genetic Insert: L3788 promoter::GFP in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21378 Copy
Species: Caenorhabditis elegans
Genetic Insert: gld-1 M1M2 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21387 Copy
Species: Caenorhabditis elegans
Genetic Insert: mCherry::histone H2B in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21386 Copy
Species: Caenorhabditis elegans
Genetic Insert: gld-1/cye-1 operon in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21388 Copy
Species: Caenorhabditis elegans
Genetic Insert: pie-1 promoter (short) in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21380 Copy
Species: Caenorhabditis elegans
Genetic Insert: phsp16.41::GFP-F64LS65T::(Gly)5Ala
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2643; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21544 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119:mCherry
Vector Backbone Description: Backbone Marker:Epicentre; Backbone Size:300; Vector Backbone:R6K origin PCR fragment; Vector Types:Worm Expression, Adds unc-119 marker to Amp resistant plasmids; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19520111
Comments: This plasmid has small deletions within the KanR gene. We generated these fragments by PCR so it is definitely possible that these were generated during amplification, these changes must not impair function as the construct is Kan resistant both as a plasmid and following recombination.
The other mismatches in 21596 are in a polylinker between the KanR gene and the unc-119 3’ UTR and should not cause any problems.
Proper citation: RRID:Addgene_21596 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmex-5 (w/o ATG)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2643; Vector Backbone:pDONR P4-P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21512 Copy
Species: Caenorhabditis elegans
Genetic Insert: (Gly)5Ala::GFP-F64LS65T::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2639; Vector Backbone:pDONR P2R-P3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21510 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmex-5::citrine (w introns)::(Gly)5Ala
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2643; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21508 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmex-5::GFP-F64LS65T::(Gly)5Ala
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2643; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21507 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmex-5::GFP (w introns)::(Gly)5Ala
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2643; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21506 Copy
Species: Caenorhabditis elegans
Genetic Insert: pie-1>mCherry::PH domain (PLC-delta)
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:5712; Vector Backbone:pBS Sk-; Vector Types:bacterial propagation; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19098902
Proper citation: RRID:Addgene_21743 Copy
Species: Caenorhabditis elegans
Genetic Insert: abu-1 promoter
Vector Backbone Description: Backbone Marker:Available at Addgene (#1494); Backbone Size:4487; Vector Backbone:pPD95_75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12186849
Comments: Addgene sequencing results show several mismatches with author's sequence.
abu-1::gfp C. elegans reporter gene. A 1.3-kb fragment of C. elegans genomic DNA immediately 5' of the predicted initiation ATG codon of abu-1 (AC3.3) was amplified by PCR using the oligonucleotides AC3.1S (5'-GGCATTGTGGCACGCATTGAACTG-3') and AC3Bam.2AS (5'-GATAGGATCCATTGTTAATATGCTTGAAGAGCTGC-3') and ligated in frame with the GFP coding region in the plasmid of pPD95.75.
Proper citation: RRID:Addgene_21824 Copy
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