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Species: Synthetic
Genetic Insert: eCFP
Vector Backbone Description: Vector Backbone:pK; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31367038
Proper citation: RRID:Addgene_176559 Copy
Species: Synthetic
Genetic Insert: eCFP
Vector Backbone Description: Vector Backbone:pM; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35314781
Proper citation: RRID:Addgene_176558 Copy
Species: Synthetic
Genetic Insert: PE2-NG variant
Vector Backbone Description: Vector Backbone:pLenti-PE2-BSD; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34446856
Proper citation: RRID:Addgene_176933 Copy
Species: Synthetic
Genetic Insert: moxGFP-P2A-T2A
Vector Backbone Description: Backbone Marker:Eric Kowarz; Backbone Size:5857; Vector Backbone:pSBbi-Pur (Addgene Plasmid #60523); Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34877484
Comments: The moxGFP-P2A-T2A construct was designed by Jorge L. Arias-Arias from Universidad de Costa Rica ([email protected]). It was codon optimized for human and mouse expression and includes two additional cloning sites (5' EcoRV - 3' SalI and 5' SmaI - 3' XbaI, see the map uploaded by the depositor) for the polycistronic expression of up to three genes: moxGFP and two more. This vector is suitable for transient transfection, but it was intended to be co-transfected with the transposase vector pCMV(CAT)T7-SB100 (Addgene Plasmid #34879) in order to generate mammalian cells with stable expression of the genes of interest. Enjoy!
Proper citation: RRID:Addgene_176893 Copy
Species: Synthetic
Genetic Insert: yeGFP
Vector Backbone Description: Backbone Marker:EUROSCARF; Backbone Size:6091; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34939781
Comments: This plasmid was used as a template to amplify the reporter cassette for the genomic integration.
Proper citation: RRID:Addgene_177163 Copy
Species: Synthetic
Genetic Insert: yeGFP
Vector Backbone Description: Backbone Marker:EUROSCARF; Backbone Size:6091; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34939781
Comments: This plasmid was used as a template to amplify the reporter cassette for the genomic integration.
Proper citation: RRID:Addgene_177162 Copy
Species: Synthetic
Genetic Insert: yeCherry
Vector Backbone Description: Backbone Marker:EUROSCARF; Backbone Size:6088; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34939781
Comments: This plasmid was used as a template to amplify the reporter cassette for the genomic integration.
Proper citation: RRID:Addgene_177165 Copy
Species: Synthetic
Genetic Insert: yeCherry
Vector Backbone Description: Backbone Marker:EUROSCARF; Backbone Size:6088; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34939781
Comments: This plasmid was used as a template to amplify the reporter cassette for the genomic integration.
Proper citation: RRID:Addgene_177164 Copy
Species: Synthetic
Genetic Insert: PCP-VP64
Vector Backbone Description: Backbone Marker:EUROSCARF; Backbone Size:7364; Vector Backbone:pRS42K; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34939781
Proper citation: RRID:Addgene_177156 Copy
Species: Synthetic
Genetic Insert: PCP-VP64
Vector Backbone Description: Backbone Marker:EUROSCARF; Backbone Size:7364; Vector Backbone:pRS42K; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34939781
Proper citation: RRID:Addgene_177157 Copy
Species: Synthetic
Genetic Insert: Tandem dimer superfolder GFP
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, dre / rox ; piggyBac; Bacterial Resistance:Ampicillin
Comments: When utilized in a reporter knocked in into a genomic locus, the stop cassette in this expression vector leaked in vivo (https://doi.org/10.6084/m9.figshare.16570695.v5).
Four nucleotides that were previously missing in the 5' end of the WPRE were added back, and SnapGene now recognizes it as WPRE.
Proper citation: RRID:Addgene_177150 Copy
Species: Synthetic
Genetic Insert: sgRNA targeting the PEAR-GFP plasmid
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35196219
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.04.26.441486v1.
Proper citation: RRID:Addgene_177183 Copy
Species: Synthetic
Genetic Insert: pegRNA targeting the PEAR-GFP plasmid
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35196219
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.04.26.441486v1.
Proper citation: RRID:Addgene_177182 Copy
Species: Synthetic
Genetic Insert: EGFP split with an intron between amino acids 95-96
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35196219
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.04.26.441486v1.
Proper citation: RRID:Addgene_177186 Copy
Species: Synthetic
Genetic Insert: hyperPE2
Vector Backbone Description: Backbone Size:3331; Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34556671
Proper citation: RRID:Addgene_177173 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-iCreERT2
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Cre/Lox, piggyBac; Bacterial Resistance:Ampicillin
Comments: Optimized T2A sequence: https://doi.org/10.6084/m9.figshare.13636142.v79.
pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSAS) (#137864, https://www.addgene.org/137864/) was updated with (1) improved Kozak consensus sequence, (2) a slightly different codon usage in the 5' linker of the T2A, (3) a silently mutated XhoI site in iCre was reverted, and (4) four nucleotides were restored to the 5' of the WPRE.
Proper citation: RRID:Addgene_177308 Copy
Species: Synthetic
Genetic Insert: ZnGreen1
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27539450
Comments: When selecting zinc biosensors for your particular application, please pay attention to the Kd. The Kd of the sensor has to match the concentration range of your particular experiments.
Proper citation: RRID:Addgene_169470 Copy
Species: Synthetic
Genetic Insert: pMag-Dre247
Vector Backbone Description: Vector Backbone:Adapted from pT2/BH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33318209
Proper citation: RRID:Addgene_169705 Copy
Species: Synthetic
Genetic Insert: Dre246-nMag-T2A-pMag-Dre247
Vector Backbone Description: Vector Backbone:Adapted from px330; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33318209
Proper citation: RRID:Addgene_169706 Copy
Species: Synthetic
Genetic Insert: a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
Vector Backbone Description: Vector Backbone:Adapted from px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33318209
Proper citation: RRID:Addgene_169708 Copy
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