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Species: M. smegmatis
Genetic Insert: MSMEG_0576
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159067 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0581
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159068 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0758
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159083 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0760
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159084 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0754
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159082 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0736
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159080 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0732
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159079 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0690
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159076 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0678
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159074 Copy
Species: Synthetic
Genetic Insert: Streptomyces codon optimized spCas9n (D10A)
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:31548381
Proper citation: RRID:Addgene_131464 Copy
Vector Backbone Description: Vector Backbone:pJMD13; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:31705217
Proper citation: RRID:Addgene_138653 Copy
Species: Other
Genetic Insert: FnCpf1
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:31090220
Proper citation: RRID:Addgene_129554 Copy
Species: Other
Genetic Insert: Sth1Cas9
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:31090220
Comments: Please note that the Addgene verified sequence differs from the depositor's reference sequence. These differences do not affect plasmid function.
Proper citation: RRID:Addgene_129552 Copy
Species: Other
Genetic Insert: SaCas9
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:31090220
Proper citation: RRID:Addgene_129553 Copy
Vector Backbone Description: Backbone Size:6440; Vector Backbone:pUC19; Vector Types:Expression vector for targeted integration; Bacterial Resistance:Apramycin
Defining Citation: PMID:34890250
Comments: For cloning in homology arms, can use Gateway, restriction ligation, or homology-based cloning such as Gibson/Infusion cloning. eGFP can be swapped for your gene of interest or can be fused (recommend homology base cloning).
Original pSelAct backbone was developed here:
Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151
pSelAct-Express was develop and tested here:
Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345
Proper citation: RRID:Addgene_174375 Copy
Species: Synthetic
Genetic Insert: attL
Vector Backbone Description: Backbone Marker:Bierman M. et al. Gene 1992 Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.; Backbone Size:3469; Vector Backbone:pOJ260; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:35456877
Proper citation: RRID:Addgene_172193 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:6573; Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:35801861
Proper citation: RRID:Addgene_190814 Copy
Species: Synthetic
Genetic Insert: dCas9-XTEN-rpoA
Vector Backbone Description: Backbone Size:6578; Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:35801861
Proper citation: RRID:Addgene_190815 Copy
Species: Synthetic
Genetic Insert: sSpCas9
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:25458909
Proper citation: RRID:Addgene_61736 Copy
Species: Synthetic
Genetic Insert: sSpCas9
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:25458909
Proper citation: RRID:Addgene_61737 Copy
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