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Species: Gallus gallus
Genetic Insert: Fusion Glycoprotein type C
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCAGGS vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21806473
Proper citation: RRID:Addgene_67512 Copy
Species: Gallus gallus
Genetic Insert: ednrbE2
Vector Backbone Description: Vector Backbone:pTK; Vector Types:Enhancer reporter construct; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31792380
Proper citation: RRID:Addgene_127772 Copy
Species: Gallus gallus
Genetic Insert: tfap2b
Vector Backbone Description: Vector Backbone:pTK; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31792380
Proper citation: RRID:Addgene_127776 Copy
Species: Gallus gallus
Genetic Insert: LH2B
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9335341
Comments: full length LH-2B.
Proper citation: RRID:Addgene_16271 Copy
Species: Gallus gallus
Genetic Insert: NgCam
Vector Backbone Description: Vector Backbone:pBeta Actin-Halo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32959500
Comments: pBa vector is susceptible to recombination and should not be grown in fast growing bacteria or cloned using recombination.
Proper citation: RRID:Addgene_162719 Copy
Species: Gallus gallus
Genetic Insert: Gallus gallus vinculin
Vector Backbone Description: Vector Backbone:pET15b/GVcl 1-1066 (Addgene #46171); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_162781 Copy
Species: Gallus gallus
Genetic Insert: ER81
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9814709
Comments: To make an antisense probe, cut with NotI and transcribe with T7.
Proper citation: RRID:Addgene_16274 Copy
Species: Gallus gallus
Genetic Insert: islet1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To make a sense probe, cut with XhoI and transcribe with T3. To make an antisense probe, cut with NotI transcribe with T7.
Proper citation: RRID:Addgene_16273 Copy
Species: Gallus gallus
Genetic Insert: LH-2A
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_16280 Copy
Species: Gallus gallus
Genetic Insert: AP-SEMA3C
Vector Backbone Description: Backbone Marker:modified from PCDNA3 see methods in associated publication.; Backbone Size:6000; Vector Backbone:pAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9331347
Proper citation: RRID:Addgene_29449 Copy
Species: Gallus gallus
Genetic Insert: AP-SEMA3A (chick)
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9331347
Proper citation: RRID:Addgene_29448 Copy
Species: Gallus gallus
Genetic Insert: SEMA3A (chick)
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:3000; Vector Backbone:pBLUESCRIPT (SK); Vector Types:pBlue for ISH; Bacterial Resistance:Ampicillin
Comments: To generate the sense probe for ISH, linearize the plasmid with XbaI and transcribe with T3 RNA polymerase. To generate the antisense probe, linearize the plasmid with NotI and transcribe with T7 RNA polymerase.
Please note that T7 is the 5' sequencing primer for the vector; however, the SEMA3A insert is cloned in reverse orientation. For cloning purposes, NotI is at the 5' end of SEMA3A while XbaI is at the 3' end of SEMA3A.
Proper citation: RRID:Addgene_29564 Copy
Species: Gallus gallus
Genetic Insert: venusYFP, TVA950, Rabies Glycoprotein
Vector Backbone Description: Backbone Size:3520; Vector Backbone:pCAG-venusYFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20797534
Comments: Please note that the Addgene sequencing result shows a CMV promoter is present. A CAG promoter was not identified as the plasmid name implies.
Proper citation: RRID:Addgene_26721 Copy
Species: Gallus gallus
Genetic Insert: c-Src
Vector Backbone Description: Backbone Marker:Addgene Plasmid 1765; Backbone Size:5558; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19573813
Comments: GenBank: V00402.1
Proper citation: RRID:Addgene_26983 Copy
Species: Gallus gallus
Genetic Insert: pyruvate kinase
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11152524
Proper citation: RRID:Addgene_24163 Copy
Species: Gallus gallus
Genetic Insert: Bromodomain containing 7
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18602913
Comments: Probe 2_93
Proper citation: RRID:Addgene_19232 Copy
Species: Gallus gallus
Genetic Insert: Alpha 2 globin
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18602913
Comments: Probe 2_89
Proper citation: RRID:Addgene_19231 Copy
Species: Gallus gallus
Genetic Insert: Survivin
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18602913
Comments: Probe 2_94
Proper citation: RRID:Addgene_19233 Copy
Species: Gallus gallus
Genetic Insert: Growth differentiation factor 10 precursor
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18602913
Comments: Probe 1_4
Proper citation: RRID:Addgene_19186 Copy
Species: Gallus gallus
Genetic Insert: CLN8
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18602913
Comments: Probe 2_29
Proper citation: RRID:Addgene_19212 Copy
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