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Species: Other
Genetic Insert: Ham1
Vector Backbone Description: Vector Backbone:pSALECT; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25790188
Proper citation: RRID:Addgene_59706 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:6215; Vector Backbone:pMZ283; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25352017
Comments: Please note that some batches of CspCI from New England Biolabs may cut inefficiently and alters the mobility of the DNA, possibly because it remains bound to the target. The two batches that are known to work are 0041303 and 0051407. This may be due to the particular target sequence present in pMZ283 and pMZ374.
Proper citation: RRID:Addgene_59896 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:6215; Vector Backbone:pMZ283; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25352017
Proper citation: RRID:Addgene_59898 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:6215; Vector Backbone:pMZ283; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25352017
Proper citation: RRID:Addgene_59897 Copy
Species: Other
Genetic Insert: ALIPHATIC AMIDASE
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3582; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25286161
Proper citation: RRID:Addgene_59836 Copy
Species: Other
Genetic Insert: ALIPHATIC AMIDASE
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3582; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25286161
Proper citation: RRID:Addgene_59839 Copy
Species: Other
Genetic Insert: ALIPHATIC AMIDASE
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3582; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25286161
Proper citation: RRID:Addgene_59841 Copy
Species: Other
Genetic Insert: ALIPHATIC AMIDASE
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3582; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25286161
Proper citation: RRID:Addgene_59840 Copy
Species: Other
Genetic Insert: TALEN-ROSA26
Vector Backbone Description: Backbone Marker:addgene; Vector Backbone:phRL-SV40; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25241166
Proper citation: RRID:Addgene_60026 Copy
Species: Other
Genetic Insert: enhanced Green Fluorescent Protein
Vector Backbone Description: Backbone Marker:Christopher Richie / OTTC / NIDA; Vector Backbone:pOTTC374 - pAAV EF1a DIO iRFP; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24746855
Proper citation: RRID:Addgene_60058 Copy
Species: Other
Genetic Insert: Integrase_9
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60580 Copy
Species: Other
Genetic Insert: T7 RNAP
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pBAD33; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23479654
Proper citation: RRID:Addgene_60717 Copy
Species: Other
Genetic Insert: dsRED
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2974; Vector Backbone:pJET1.2; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25324299
Comments: The plasmid contains an IS4-like element that does not interfere with function as described in the associated publication.
Proper citation: RRID:Addgene_60944 Copy
Species: Other
Genetic Insert: vas-phiC31 integrase.NLS
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript II; Vector Types:RMCE; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25324299
Proper citation: RRID:Addgene_60948 Copy
Species: Other
Genetic Insert: Chloramphenicol acetyltransferase
Vector Backbone Description: Backbone Marker:Ambion; Vector Backbone:pAMB; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26781350
Proper citation: RRID:Addgene_61002 Copy
Species: Other
Genetic Insert: LovTAP N538E
Vector Backbone Description: Vector Backbone:pCAL-N; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20562867
Proper citation: RRID:Addgene_61431 Copy
Species: Other
Genetic Insert: hSaCas9
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25830891
Comments: Note: This plasmid does not have an sgRNA expression cassette. Please refer to the associated publication, as well as the AAV portion of http://www.addgene.org/crispr/zhang/ , for more information on expressing sgRNAs.
Proper citation: RRID:Addgene_61594 Copy
Species: Other
Genetic Insert: N-mCherry-ME(pS)VD
Vector Backbone Description: Backbone Size:3937; Vector Backbone:pNAS1B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25272187
Proper citation: RRID:Addgene_61966 Copy
Species: Other
Genetic Insert: mRFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:25422271
Proper citation: RRID:Addgene_62247 Copy
Species: Other
Genetic Insert: mRFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:25422271
Proper citation: RRID:Addgene_62248 Copy
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