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Species: M. smegmatis
Genetic Insert: MSMEG_0754
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159082 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0736
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159080 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0732
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159079 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0690
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159076 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_0678
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
Proper citation: RRID:Addgene_159074 Copy
Vector Backbone Description: Vector Backbone:pIJ8668; Vector Types:Bacterial Expression, Streptomyces expression; Bacterial Resistance:Apramycin
Proper citation: RRID:Addgene_78576 Copy
Species: Synthetic
Genetic Insert: attL
Vector Backbone Description: Backbone Marker:Bierman M. et al. Gene 1992 Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.; Backbone Size:3469; Vector Backbone:pOJ260; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:35456877
Proper citation: RRID:Addgene_172193 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:6573; Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:35801861
Proper citation: RRID:Addgene_190814 Copy
Species: Synthetic
Genetic Insert: dCas9-XTEN-rpoA
Vector Backbone Description: Backbone Size:6578; Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:35801861
Proper citation: RRID:Addgene_190815 Copy
Vector Backbone Description: Backbone Size:6440; Vector Backbone:pUC19; Vector Types:Expression vector for targeted integration; Bacterial Resistance:Apramycin
Defining Citation: PMID:34890250
Comments: For cloning in homology arms, can use Gateway, restriction ligation, or homology-based cloning such as Gibson/Infusion cloning. eGFP can be swapped for your gene of interest or can be fused (recommend homology base cloning).
Original pSelAct backbone was developed here:
Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151
pSelAct-Express was develop and tested here:
Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345
Proper citation: RRID:Addgene_174375 Copy
Species: Other
Genetic Insert: streptomyces codon optimized spCas9, sgRNA casette, and ScaligD
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:25806970
Comments: Please note that an IS1 transposase sequence was found downstream of Cas9 during Addgene's quality control. The depositor noted that this sequence should NOT affect plasmid function.
Proper citation: RRID:Addgene_125688 Copy
Species: Other
Genetic Insert: streptomyces codon optimized spdCas9, sgRNA casette
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:25806970
Proper citation: RRID:Addgene_125687 Copy
Species: Synthetic
Genetic Insert: mCherry, aac(3)IV, tsr
Vector Backbone Description: Vector Backbone:pSG5; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Comments: Mobilizable, stable replicating expression vector for Streptomyces, strong constitutive SF14* promoter, codon optimized Strep-tagII-mCherry gene, tsr, aac(3)IV
Proper citation: RRID:Addgene_115683 Copy
Species: Streptomyces
Genetic Insert: gusA, aac(3)IV, tsr
Vector Backbone Description: Vector Backbone:pSG5; Vector Types:Gene knock out; Bacterial Resistance:Apramycin
Comments: Thermosensitive replication (< 37°C) in Streptomyces, Detection of single/double cross over by X-gluc colour reaction , aac(3)IV
Proper citation: RRID:Addgene_115678 Copy
Vector Backbone Description: Backbone Size:5613; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126044 Copy
Vector Backbone Description: Backbone Size:5681; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126595 Copy
Vector Backbone Description: Backbone Size:5220; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126596 Copy
Species: Synthetic
Genetic Insert: tcp830
Vector Backbone Description: Backbone Size:4460; Vector Backbone:pSET152; Vector Types:Streptomyce integration vector derived from phiC31 int/attP; Bacterial Resistance:Apramycin
Defining Citation: PMID:15917435
Comments: For research using this plasmid, please acknowledge the depositing laboratory and associated citation.
Proper citation: RRID:Addgene_127088 Copy
Vector Backbone Description: Vector Backbone:pJMD13; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:31705217
Proper citation: RRID:Addgene_138653 Copy
Species: Other
Genetic Insert: FnCpf1
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:31090220
Proper citation: RRID:Addgene_129554 Copy
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