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  • Bacterial Resistance:apramycin (facet)

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pMSR:MSMEG_0576:Dendra
 
Resource Report
Resource Website 		RRID:Addgene_159067 MSMEG_0576 M. smegmatis Apramycin PMID:33653882 NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin 2026-08-15 01:08:26 0
pMSR:MSMEG_0581:Dendra
 
Resource Report
Resource Website 		RRID:Addgene_159068 MSMEG_0581 M. smegmatis Apramycin PMID:33653882 NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin 2026-08-15 01:08:24 0
pMSR:MSMEG_0758:Dendra
 
Resource Report
Resource Website 		RRID:Addgene_159083 MSMEG_0758 M. smegmatis Apramycin PMID:33653882 NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin 2026-08-15 01:08:24 0
pMSR:MSMEG_0760:Dendra
 
Resource Report
Resource Website 		RRID:Addgene_159084 MSMEG_0760 M. smegmatis Apramycin PMID:33653882 NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin 2026-08-15 01:08:26 0
pMSR:MSMEG_0754:Dendra
 
Resource Report
Resource Website 		RRID:Addgene_159082 MSMEG_0754 M. smegmatis Apramycin PMID:33653882 NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin 2026-08-15 01:08:24 0
pMSR:MSMEG_0736:Dendra
 
Resource Report
Resource Website 		RRID:Addgene_159080 MSMEG_0736 M. smegmatis Apramycin PMID:33653882 NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin 2026-08-15 01:08:24 0
pMSR:MSMEG_0732:Dendra
 
Resource Report
Resource Website 		RRID:Addgene_159079 MSMEG_0732 M. smegmatis Apramycin PMID:33653882 NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin 2026-08-15 01:08:26 0
pMSR:MSMEG_0690:Dendra
 
Resource Report
Resource Website 		RRID:Addgene_159076 MSMEG_0690 M. smegmatis Apramycin PMID:33653882 NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin 2026-08-15 01:08:26 0
pMSR:MSMEG_0678:Dendra
 
Resource Report
Resource Website 		RRID:Addgene_159074 MSMEG_0678 M. smegmatis Apramycin PMID:33653882 NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5; Bacterial Resistance:Apramycin 2026-08-15 01:08:24 0
pCRISPR-aBEST
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_131464 Streptomyces codon optimized spCas9n (D10A) Synthetic Apramycin PMID:31548381 Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin 2026-08-15 01:05:17 3
pJMD13
 
Resource Report
Resource Website 		RRID:Addgene_138653 Apramycin PMID:31705217 Vector Backbone:pJMD13; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-08-15 01:06:25 0
pCRISPomyces-FnCpf1
 
Resource Report
Resource Website 		RRID:Addgene_129554 FnCpf1 Other Apramycin PMID:31090220 Vector Backbone:pKC1139; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-08-15 01:04:58 0
pCRISPomyces-Sth1Cas9
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_129552 Sth1Cas9 Other Apramycin PMID:31090220 Please note that the Addgene verified sequence differs from the depositor's reference sequence. These differences do not affect plasmid function. Vector Backbone:pKC1139; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-08-15 01:04:58 1
pCRISPomyces-SaCas9
 
Resource Report
Resource Website 		RRID:Addgene_129553 SaCas9 Other Apramycin PMID:31090220 Vector Backbone:pKC1139; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-08-15 01:04:59 0
pSelAct-Express
 
Resource Report
Resource Website 		RRID:Addgene_174375 Apramycin PMID:34890250 For cloning in homology arms, can use Gateway, restriction ligation, or homology-based cloning such as Gibson/Infusion cloning. eGFP can be swapped for your gene of interest or can be fused (recommend homology base cloning). Original pSelAct backbone was developed here: Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151 pSelAct-Express was develop and tested here: Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345 Backbone Size:6440; Vector Backbone:pUC19; Vector Types:Expression vector for targeted integration; Bacterial Resistance:Apramycin 2026-08-15 01:23:40 0
pEA02
 
Resource Report
Resource Website 		RRID:Addgene_172193 attL Synthetic Apramycin PMID:35456877 Backbone Marker:Bierman M. et al. Gene 1992 Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.; Backbone Size:3469; Vector Backbone:pOJ260; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin 2026-08-15 01:23:43 0
pJEC726
 
Resource Report
Resource Website 		RRID:Addgene_190814 dCas9 Synthetic Apramycin PMID:35801861 Backbone Marker:Addgene; Backbone Size:6573; Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin S. pyogenes dCas9 codon optimized for Streptomyces expression. The D10A and H840A mutations were introduced to convert Cas9 to dCas9. 2026-08-15 01:23:55 0
pJEC819
 
Resource Report
Resource Website 		RRID:Addgene_190815 dCas9-XTEN-rpoA Synthetic Apramycin PMID:35801861 Backbone Size:6578; Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin 2026-08-15 01:23:55 0
pCRISPomyces-1
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_61736 sSpCas9 Synthetic Apramycin PMID:25458909 Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin 2026-08-15 01:17:45 1
pCRISPomyces-2
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_61737 sSpCas9 Synthetic Apramycin PMID:25458909 Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin 2026-08-15 01:17:46 19

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