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ML40K1
 
Resource Report
Resource Website 		RRID:Addgene_61920 none Chloramphenicol PMID:21925267 Genotype = cyo ilvE avtA aspC hisG argH metA lysA Precursor strain = ML31 Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Arg, Met, Lys ML40(K1) contains an extra pACYC plasmid carrying the cat cassette plus extra tRNA genes (argU, ileY, leuW) from Agilent Technologies Inc. *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. Vector Backbone:none; Vector Types:; Bacterial Resistance:Chloramphenicol 2026-08-15 01:17:47 0
pMU102
 
Resource Report
Resource Website 		RRID:Addgene_61805 Chloramphenicol PMID:22578246 Backbone Marker:Bacillus Genetic Stock Center; Backbone Size:4217; Vector Backbone:pNW33N; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:17:46 0
pACYC-GST
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_62329 Chloramphenicol Backbone Marker:Structural Genomics Consortium; Backbone Size:8362; Vector Backbone:pACYC-LIC+; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:17:52 1
pCas9-CR4
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_62655 Cas9 nuclease Chloramphenicol PMID:26463009 Please note that this plasmid runs as a dimer (>13kb). While this may reduce DNA yield, the plasmid still functions as expected. Vector Backbone:pdCas9-bacteria; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:17:54 23
p0521s
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_62862 Chloramphenicol PMID:25897682 Contains yeast URA3 and HIS3 genes for selection in yeast. Contains ShBle cassette for selection in Phaeodactylum tricornutum. Depositor states that the I-SceI restriction site in this plasmid (5’-AGTTACGCTAGGGATAACAGGTAATATAG) varies from the NEB listed recognition sequence and may reduce the efficiency of cleavage using this enzyme. Vector Backbone:pCC1BAC; Vector Types:Synthetic Biology, Episomal vector for Phaeodactylum tricornutum; Bacterial Resistance:Chloramphenicol 2026-08-15 01:17:56 2
pAV10.F3
 
Resource Report
Resource Website 		RRID:Addgene_63199 Chloramphenicol PMID:25756291 Backbone Marker:BioBrick ; Backbone Size:4100; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:17:59 0
pVRc02_915
 
Resource Report
Resource Website 		RRID:Addgene_49729 AS02_915 Shewanella amazonensis SB2B Chloramphenicol PMID:24169405 Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol Codon optimized for E.coli 2026-08-15 01:16:01 0
pACM4-ACA
 
Resource Report
Resource Website 		RRID:Addgene_49809 ACP transacylase E. coli Chloramphenicol PMID:23361000 Backbone Size:3956; Vector Backbone:pACM4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol Silent site-directed mutagenesis to remove SalI site (GTCGAC to GTGGAC) 2026-08-15 01:16:02 0
pVRc37_2513
 
Resource Report
Resource Website 		RRID:Addgene_49751 AS37_2513 Burkholderia thailandensis E264 Chloramphenicol PMID:24169405 Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol Codon optimized for E.coli 2026-08-15 01:16:03 0
pVRc35_3582
 
Resource Report
Resource Website 		RRID:Addgene_49750 AS35_3582 Pseudomonas aeruginosa PAO1 Chloramphenicol PMID:24169405 Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol Codon optimized for E.coli 2026-08-15 01:16:02 0
pVRc27_4265
 
Resource Report
Resource Website 		RRID:Addgene_49745 AS27_4265 Streptomyces coelicolor A3(2) Chloramphenicol PMID:24169405 Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol Codon optimized for E.coli 2026-08-15 01:16:03 0
pVRc30_35
 
Resource Report
Resource Website 		RRID:Addgene_49746 AS30_35 Bacillus subtilis subsp. subtilis str. 168 Chloramphenicol PMID:24169405 Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol Codon optimized for E.coli 2026-08-15 01:16:02 0
pVRc22_1147
 
Resource Report
Resource Website 		RRID:Addgene_49740 AS22_1147 Xanthomonas axonopodis pv. citri str. 306 Chloramphenicol PMID:24169405 Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol Codon optimized for E.coli 2026-08-15 01:16:02 0
pVRc20_992
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_49739 AS20_992 Pseudomonas fluorescens Pf-5 Chloramphenicol PMID:24169405 Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol Codon optimized for E.coli 2026-08-15 01:16:03 1
pVRc11_987
 
Resource Report
Resource Website 		RRID:Addgene_49733 AS11_987 Vibrio parahaemolyticus RIMD 2210633 Chloramphenicol PMID:24169405 Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol Codon optimized for E.coli 2026-08-15 01:16:03 0
pVRc04_1609
 
Resource Report
Resource Website 		RRID:Addgene_49732 AS04_1609 Chlorobium tepidum TLS Chloramphenicol PMID:24169405 Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol Codon optimized for E.coli 2026-08-15 01:16:01 0
pVRc03_1244
 
Resource Report
Resource Website 		RRID:Addgene_49731 AS03_1244 Porphyromonas gingivalis W83 Chloramphenicol PMID:24169405 Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol Codon optimized for E.coli 2026-08-15 01:16:01 0
pVRc03_1198
 
Resource Report
Resource Website 		RRID:Addgene_49730 AS03_1198 Bacteroides thetaiotaomicron VPI-5482 Chloramphenicol PMID:24169405 Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol Codon optimized for E.coli 2026-08-15 01:16:03 0
pBL4
 
Resource Report
Resource Website 		RRID:Addgene_50548 CcaS Synechocystis sp. 6803 Chloramphenicol PMID:24608181 Note that Addgene NGS identified a nonsense mutation in the CcaS insert Backbone Marker:Clontech; Backbone Size:1900; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:16:09 0
pCph8
 
Resource Report
Resource Website 		RRID:Addgene_50552 Cph8 Synthetic Chloramphenicol PMID:24608181 Encodes the Cph8 (Cph1/EnvZ fusion) light sensor kinase/phosphatase downstream of the pLTetO-1 promoter. Requires pPLPCB(S)t or some other source of TetR Requires PCB to function Does NOT include the response regulator. Backbone Marker:Clontech; Backbone Size:1995; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:16:07 0

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