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Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21925267
Comments: Genotype = cyo ilvE avtA aspC hisG argH metA lysA
Precursor strain = ML31
Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Arg, Met, Lys
ML40(K1) contains an extra pACYC plasmid carrying the cat cassette plus extra tRNA genes (argU, ileY, leuW) from Agilent Technologies Inc.
*In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)].
#In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)].
Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system.
Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain.
Proper citation: RRID:Addgene_61920 Copy
Vector Backbone Description: Backbone Marker:Bacillus Genetic Stock Center; Backbone Size:4217; Vector Backbone:pNW33N; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:22578246
Proper citation: RRID:Addgene_61805 Copy
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium; Backbone Size:8362; Vector Backbone:pACYC-LIC+; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_62329 Copy
Genetic Insert: Cas9 nuclease
Vector Backbone Description: Vector Backbone:pdCas9-bacteria; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26463009
Comments: Please note that this plasmid runs as a dimer (>13kb). While this may reduce DNA yield, the plasmid still functions as expected.
Proper citation: RRID:Addgene_62655 Copy
Vector Backbone Description: Vector Backbone:pCC1BAC; Vector Types:Synthetic Biology, Episomal vector for Phaeodactylum tricornutum; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25897682
Comments: Contains yeast URA3 and HIS3 genes for selection in yeast. Contains ShBle cassette for selection in Phaeodactylum tricornutum.
Depositor states that the I-SceI restriction site in this plasmid (5’-AGTTACGCTAGGGATAACAGGTAATATAG) varies from the NEB listed recognition sequence and may reduce the efficiency of cleavage using this enzyme.
Proper citation: RRID:Addgene_62862 Copy
Vector Backbone Description: Backbone Marker:BioBrick ; Backbone Size:4100; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25756291
Proper citation: RRID:Addgene_63199 Copy
Species: Shewanella amazonensis SB2B
Genetic Insert: AS02_915
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49729 Copy
Species: E. coli
Genetic Insert: ACP transacylase
Vector Backbone Description: Backbone Size:3956; Vector Backbone:pACM4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23361000
Proper citation: RRID:Addgene_49809 Copy
Species: Burkholderia thailandensis E264
Genetic Insert: AS37_2513
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49751 Copy
Species: Pseudomonas aeruginosa PAO1
Genetic Insert: AS35_3582
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49750 Copy
Species: Streptomyces coelicolor A3(2)
Genetic Insert: AS27_4265
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49745 Copy
Species: Bacillus subtilis subsp. subtilis str. 168
Genetic Insert: AS30_35
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49746 Copy
Species: Xanthomonas axonopodis pv. citri str. 306
Genetic Insert: AS22_1147
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49740 Copy
Species: Pseudomonas fluorescens Pf-5
Genetic Insert: AS20_992
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49739 Copy
Species: Vibrio parahaemolyticus RIMD 2210633
Genetic Insert: AS11_987
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49733 Copy
Species: Chlorobium tepidum TLS
Genetic Insert: AS04_1609
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49732 Copy
Species: Porphyromonas gingivalis W83
Genetic Insert: AS03_1244
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49731 Copy
Species: Bacteroides thetaiotaomicron VPI-5482
Genetic Insert: AS03_1198
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49730 Copy
Species: Synechocystis sp. 6803
Genetic Insert: CcaS
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:1900; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24608181
Comments: Note that Addgene NGS identified a nonsense mutation in the CcaS insert
Proper citation: RRID:Addgene_50548 Copy
Species: Synthetic
Genetic Insert: Cph8
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:1995; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24608181
Comments: Encodes the Cph8 (Cph1/EnvZ fusion) light sensor kinase/phosphatase downstream of the pLTetO-1 promoter.
Requires pPLPCB(S)t or some other source of TetR
Requires PCB to function
Does NOT include the response regulator.
Proper citation: RRID:Addgene_50552 Copy
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