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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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RF10 Resource Report Resource Website 1+ mentions |
RRID:Addgene_62076 | none | None | PMID:26577727 | Genotype = lysA Precursor strain = BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Lys Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:17:49 | 2 | ||
|
HL2483 Resource Report Resource Website |
RRID:Addgene_62819 | see comments | None | PMID:26261213 | MG1655 + lacIq intergrated at intS + Pcon::TetR inserted into the chromosome at galK + ΔglgCAP + ΔcsrB + ΔcsrC + ΔcsrD | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2026-08-15 01:17:56 | 0 | ||
|
HL 1951 Resource Report Resource Website |
RRID:Addgene_69773 | None | PMID:27084942 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2026-08-15 01:19:01 | 0 | ||||
|
HL 6779 Resource Report Resource Website |
RRID:Addgene_69783 | None | PMID:27084942 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2026-08-15 01:18:55 | 0 | ||||
|
HL 6776 Resource Report Resource Website |
RRID:Addgene_69780 | None | PMID:27084942 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2026-08-15 01:18:55 | 0 | ||||
|
HL 6825 Resource Report Resource Website |
RRID:Addgene_69784 | None | PMID:27084942 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2026-08-15 01:18:55 | 0 | ||||
|
CY008 Resource Report Resource Website |
RRID:Addgene_72401 | None | PMID:26315440 | No antibiotic resistance. | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:19:19 | 0 | |||
|
Strain SIJ_488 Resource Report Resource Website 1+ mentions |
RRID:Addgene_68246 | arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI. | None | PMID:26643270 | E. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI. | Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:18:46 | 1 | ||
|
EcAR7 Resource Report Resource Website 1+ mentions |
RRID:Addgene_52055 | None | None | PMID:22982858 | We recommend growth of the EcAR7 strain at 30 °C in LB supplemented with 0.08% glucose. The EcAR7 strains normally take 1.5-‐2 days to grow at 30°C, after transformation or streaking the strain on an agar plate (with the appropriate antibiotics). See supplemental information from referenced article for protocol on creating EcAR7 from EcNR2. | Vector Backbone:None; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:16:19 | 5 | ||
|
Oplac2 (37)-KI Resource Report Resource Website |
RRID:Addgene_52702 | Oplac2 (37)-KI strain | None | PMID:22605776 | To test whether the mRNA secondary structure of our codon redesigned sequences affected expression, we substituted the first 37 nucleotides of KIlac with those of OpLac2. | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:16:25 | 0 | ||
|
delta-YA Resource Report Resource Website |
RRID:Addgene_52708 | ΔYA strain | None | PMID:22605776 | lacY and lacA deletion | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:16:25 | 0 | ||
|
delta-ZA Resource Report Resource Website |
RRID:Addgene_52707 | ΔZA strain | None | PMID:22605776 | lacZ and lacA deletion | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:16:25 | 0 | ||
|
W999L Resource Report Resource Website |
RRID:Addgene_52713 | W999L strain | None | PMID:22605776 | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:16:25 | 0 | |||
|
E537Q Resource Report Resource Website |
RRID:Addgene_52714 | E537Q strain | None | PMID:22605776 | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:16:25 | 0 | |||
|
G794A Resource Report Resource Website |
RRID:Addgene_52711 | G794A strain | None | PMID:22605776 | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:16:25 | 0 | |||
|
W999F Resource Report Resource Website |
RRID:Addgene_52712 | W999F strain | None | PMID:22605776 | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:16:30 | 0 | |||
|
delta-ZYA Resource Report Resource Website |
RRID:Addgene_52695 | ΔZYA strain | None | PMID:22605776 | MG1655 was transformed with pKD46 (carrying phage lambda Red recombinase) and a PCR fragment encoding a kanamycin resistance gene with flanking nucleotide sequences homologous to those flanking the lac operon. Upon recombination and selection for resistant strains, the kanamycin-resistant gene was eliminated following transformation with pCP20 (encoding the FLP recombinase), which is subsequently cured by growth at 30°C. The deletion in our ΔZYA strain started 20bp upstream of lacI and ran 40bp downstream of lacA. | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:16:25 | 0 | ||
|
OpLac1-delta-6 Resource Report Resource Website |
RRID:Addgene_52698 | OpLac1-Δ6 strain | None | PMID:22605776 | Oplac1Δ6 were erroneously synthesized missing the first 6 nucleotides of Oplac1. These deletions correspond to the first 2 N-terminal amino acid residues (Methionine and Threonine), and instead begin at the Methionine at position 3. | Vector Backbone:na; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:16:25 | 0 | ||
|
HL 1285 Resource Report Resource Website |
RRID:Addgene_52942 | none | E.coli | None | PMID:25087841 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2026-08-15 01:16:31 | 0 | ||
|
HL3721 Resource Report Resource Website |
RRID:Addgene_52950 | none | E.coli | None | PMID:23878244 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | 2026-08-15 01:16:31 | 0 |
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