Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|
|
HL 2028 Resource Report Resource Website |
RRID:Addgene_61163 | See comments | None | PMID:22833608 | HL1745 + PLlacO-1::T710::yfp intergrated at galK+ ∆lacI | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | Addgene | 2026-09-26 02:35:52 | 0 | ||
|
HL 1745 Resource Report Resource Website |
RRID:Addgene_61160 | See comments | None | PMID:22833608 | MG1655 + PLlacO-1::T710::cfp intergrated at intS | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | Addgene | 2026-09-26 02:35:52 | 0 | ||
|
HL 5220 Resource Report Resource Website |
RRID:Addgene_61164 | See comments | None | PMID:22833608 | MG1655 + PLlacO-1::T710::yfp intergrated at arpB + ∆lacI | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | Addgene | 2026-09-26 02:35:52 | 0 | ||
|
E. coli K-12 MG1655 RARE Resource Report Resource Website 1+ mentions |
RRID:Addgene_61440 | None | PMID:25076127 | This strain carries the DE3 lysogen. | Backbone Size:4600000; Vector Backbone:E. coli K-12 MG1655 genome; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:35:56 | 8 | |||
|
ML14 Resource Report Resource Website |
RRID:Addgene_61911 | none | None | PMID:21925267 | Genotype = tyrA Precursor strain = C43(DE3) modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Tyr) Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:00 | 0 | ||
|
ML17 Resource Report Resource Website 1+ mentions |
RRID:Addgene_61912 | none | None | PMID:21925267 | Genotype = glnA Precursor strain = C43(DE3) modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = Gln Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:00 | 1 | ||
|
ML36 Resource Report Resource Website |
RRID:Addgene_61919 | none | None | PMID:21925267 | Genotype = cyo ilvE avtA aspC hisG metA Precursor strain = ML23 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Met *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:01 | 0 | ||
|
ML24 Resource Report Resource Website |
RRID:Addgene_61915 | none | None | PMID:21925267 | Genotype = cyo ilvE avtA aspC hisG asnA Precursor strain = ML23 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:00 | 0 | ||
|
ML25 Resource Report Resource Website |
RRID:Addgene_61916 | none | None | PMID:21925267 | Genotype = cyo ilvE avtA aspC hisG asnA asnB Precursor strain = ML24 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Asn *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:00 | 0 | ||
|
ML42 Resource Report Resource Website |
RRID:Addgene_61921 | none | None | PMID:21925267 | Genotype = cyo ilvE avtA aspC hisG argH metA lysA thrC asnB Precursor strain = ML41 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Arg, Met, Lys, Thr *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:01 | 0 | ||
|
RF11 Resource Report Resource Website 1+ mentions |
RRID:Addgene_61961 | none | None | PMID:26577727 | Genotype = metA Precursor strain = C43(DE3) Selective amino acid labeling (and/or requirement) = Met Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:01 | 1 | ||
|
RF2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_62070 | none | None | PMID:26577727 | Genotype = thrC Precursor strain = BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Thr Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:02 | 1 | ||
|
RF12 Resource Report Resource Website 1+ mentions |
RRID:Addgene_62077 | none | None | PMID:26577727 | Genotype = trpA trpB Precursor strain = BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Trp## ##RF12 strain requires Trp for growth in M63 minimal medium. For effective Trp labeling of a recombinant protein, the presence of 0.4-1 mM Tyr in growth medium to repress the tyrB gene is recommended. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:02 | 2 | ||
|
RF5 Resource Report Resource Website |
RRID:Addgene_62073 | none | None | PMID:26577727 | Genotype = aspC tyrB hisG Precursor strain = RF4 modified from the parent Escherichia coli BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Asp++++, Tyr, (Phe), His ++++RF4, RF5, and RF13 strains have knockouts in both aspC and tyrB genes and therefore require the presence of L-Asp (but not L-Glu) for growth in M63 minimal medium. These strains can be used for selective labeling of selected aromatic amino acid(s) such as Tyr (RF4, RF5, RF13) and/or Trp (RF13). We have NOT tested if these strains could also be used for selective Asp labeling studies (under investigation). Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:02 | 0 | ||
|
RF10 Resource Report Resource Website 1+ mentions |
RRID:Addgene_62076 | none | None | PMID:26577727 | Genotype = lysA Precursor strain = BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Lys Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:02 | 2 | ||
|
HL2483 Resource Report Resource Website |
RRID:Addgene_62819 | see comments | None | PMID:26261213 | MG1655 + lacIq intergrated at intS + Pcon::TetR inserted into the chromosome at galK + ΔglgCAP + ΔcsrB + ΔcsrC + ΔcsrD | Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:10 | 0 | ||
|
MG1655 Z1 malE clpA Resource Report Resource Website |
RRID:Addgene_65918 | MG1655 Z1 malE clpA | None | PMID:26900850 | F-, lambda-, rph-1, laciq, PN25-tetR, SpR, malE-, clpA- | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:41 | 0 | ||
|
MG1655 Z1 malE aat Resource Report Resource Website |
RRID:Addgene_65917 | MG1655 Z1 malE aat | None | PMID:26900850 | F-, lambda-, rph-1, laciq, PN25-tetR, SpR, malE-, aat- | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:36:41 | 0 | ||
|
Strain SIJ_488 Resource Report Resource Website 1+ mentions |
RRID:Addgene_68246 | arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI. | None | PMID:26643270 | E. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI. | Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None | Addgene | 2026-09-26 02:37:01 | 1 | ||
|
HL 1951 Resource Report Resource Website |
RRID:Addgene_69773 | None | PMID:27084942 | Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None | Addgene | 2026-09-26 02:37:15 | 0 |
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
