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  • Organism:mus musculus (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pcDNA3-mouse PKA-RIbeta-mEGFP
 
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RRID:Addgene_45526 PKA regulatory subunit RI beta tagged by mEGFP Mus musculus Ampicillin PMID:19447092 Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:24 1
pcDNA4/TO mTRPM7
 
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RRID:Addgene_45482 TRPM7 Mus musculus Ampicillin PMID:11385574 For the purpose of expressing LTRPC7 in eukaryotic cells, the depositing lab used PCR to produce an epitope tagged expression construct from two overlapping murine LTRPC7 clones. The LTRPC7 coding sequence was modified by removing the initiating methionine and replacing it with a sequence encoding a Kozak sequence, the FLAG tag and the additional sequence GCGGCCGCAT, and by placing a SpeI site just after the stop codon. These modifications result in an expressed protein which started with the following amino acid sequence: MGDYKDDDDKRPH followed by the murine LTRPC7 coding sequence starting at the second amino acid. This construct is expressed from the pcDNA4/TO vector which provides tetracycline-controlled expression from a CMV promotor. Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA4/TO (modified); Vector Types:Mammalian Expression, Tetracycline Inducible; Bacterial Resistance:Ampicillin 2026-08-15 01:15:24 2
pcDNA3.1-Flag-PGC-1alpha1
 
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RRID:Addgene_45501 PGC-1alpha Isoform1 Mus musculus Ampicillin PMID:23217713 Note that Addgene NGS results identified a Q339K mutation relative to the canonical GenBank sequence. This mutation has not been reported to affect function. Backbone Size:5424; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Q339K relative to NM_008904 2026-08-15 01:15:24 1
pCAGGS-Hoxc6
 
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RRID:Addgene_45553 Hoxc6 Mus musculus Ampicillin PMID:23359544 Backbone Size:4800; Vector Backbone:pCAGGs; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:15:25 0
pCMV-Kl-EGFP
 
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RRID:Addgene_45532 alpha Klotho Mus musculus Ampicillin and Kanamycin PMID:26881702 Backbone Size:5710; Vector Backbone:pCR3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:15:24 1
pD40-His/V5-c-Myc
 
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RRID:Addgene_45597 c-Myc Mus musculus Ampicillin PMID:15048125 Alternate plasmid names: pD40-His-c-Myc; pcDNA-DEST40 Myc wt This His6-tagged c-Myc plasmid was created by PCR amplification of the coding sequence for wild-type murine c-Myc2 from the CMVMyc plasmid (Hann et al., Genes Dev. 1994, PMID: 7958908) with a CACC 5′ extension added to the 5′ primer for cloning into the TOPO entry clone from Invitrogen (Carlsbad, CA). This sequence was then moved into the pDEST-40 mammalian expression vector using Gateway technology (Invitrogen), creating pD40-His-c-Myc. For plasmid usage information, see the associated article (Yeh et al. Nat Cell Biol. 2004) as well as Arnold et al. EMBO 2009 (PMID: 19131971). Backbone Marker:Invitrogen; Backbone Size:7143; Vector Backbone:pcDNA DEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:25 9
pCRII-Topo Lpl in situ probe
 
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RRID:Addgene_45630 Lpl in situ probe Mus musculus Ampicillin PMID:19793993 The Lpl fragment was amplified by RT-PCR using the following primer pair: TGCTGTGCAAAGAGAAGAGC CGGACACAAAGTTAGCACCA For in vitro transcription, use the NotI restriction digest and Sp6 promoter for sense probe generation and the HindIII restriction digest and T7 promoter for antisense probe generation. Please note that Addgene's sequencing results match bp# 3249-3900 of NM_008509.2, not bp# 3169-3826 as indicated on plasmid map. The plasmid functions as described in the associated publication. Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin fragment contains bp# 3249-3900 of NM_008509.2 2026-08-15 01:15:25 0
pCRII-Topo Nectin-3 in situ probe
 
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RRID:Addgene_45633 Nectin-3 in situ probe Mus musculus Ampicillin PMID:19793993 The Nectin-3 fragment was amplified by RT-PCR using the following primer pair: AAACAACCTGATCCGCAAAG CAGTGAAAACTGTAAAGCAGCTC For in vitro transcription, use the BamHI restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and Sp6 promoter for antisense probe generation. Please note that Addgene's sequencing results match bp# 1626-2039 of NM_021495, not bp# 1624-2036 as indicated on plasmid map. The plasmid functions as described in the associated publication. Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin fragment contains bp# 1626-2039 of NM_021495 2026-08-15 01:15:25 0
pCRII-Topo Ptn in situ probe
 
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RRID:Addgene_45632 Ptn in situ probe Mus musculus Ampicillin PMID:19793993 The Ptn fragment was amplified by RT-PCR using the following primer pair: GCCTACCCGTCCAAATATCC GCCAGTTCTGGTCTTCAAGG For in vitro transcription, use the NotI restriction digest and SP6 promoter for sense probe generation and the HindIII restriction digest and T7 promoter for antisense probe generation. Please note that Addgene's sequencing results match bp# 1241-1830 of NM_008973, not bp# 1238-1827 as indicated on plasmid map. The plasmid functions as described in the associated publication. Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin fragment contains bp# 1241-1830 of NM_008973 2026-08-15 01:15:25 0
pCRII-Topo Gp88 in situ probe
 
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RRID:Addgene_45628 Gpr88 in situ probe Mus musculus Ampicillin PMID:19793993 The Gpr88 fragment was amplified by RT-PCR using the following primer pair: CAAATGAAACCAATGGTCAGG TATCTGTTTCCCGTGTCTCC For in vitro transcription, use the HindIII restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and Sp6 promoter for antisense probe generation. Please note that Addgene's sequencing results match bp# 2742-3255 of AB042408.1 or bp# 2772-3285 of NM_022427, not bp# 2742-3255 of NM_022427 as indicated on plasmid map. The plasmid functions as described in the associated publication. Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin fragment contains bp# 2742-3255 of AB042408.1 or bp# 2772-3285 of NM_022427 2026-08-15 01:15:25 0
pCRII-Topo Foxp2 in situ probe
 
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RRID:Addgene_45620 Foxp2 in situ probe Mus musculus Ampicillin PMID:16157277 The Fox2p fragment was amplified by RT-PCR using the following primer pair: CAGTGTGGACTGTGGACGAA TTCCAGGTCCTCAGATAAAGG For in vitro transcription, use the HindIII restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and SP6 promoter for antisense probe generation. Please note that Addgene's sequencing results match bp#1709-2142 of AF339106, not bp# 1707-2142 as indicated on plasmid map. The plasmid functions as described in the associated publication. Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin fragment contains bp#1709-2142 of AF339106 2026-08-15 01:15:25 0
pCRII-Topo Tcrb-V13 in situ probe
 
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RRID:Addgene_45622 Tcrb-V13 in situ probe Mus musculus Ampicillin PMID:16157277 The Tcrb-V13 fragment was amplified by RT-PCR using the following primer pair: CTGAGCTGGTGGGTGAATG AAGGTGTCAACGAGGAAGGA For in vitro transcription, use the NotI restriction digest and Sp6 promoter for sense probe generation and the HindIII restriction digest and T7 promoter for antisense probe generation. Please note that Addgene's sequencing results match bp# 533-1058 of X67128, not bp# 532-1059 as indicated on plasmid map. The plasmid functions as described in the associated publication. Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin fragment contains bp# 533-1058 of X67128 2026-08-15 01:15:25 0
pCRII-Topo Plexin D1 in situ probe
 
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RRID:Addgene_45621 Plexin D1 in situ probe Mus musculus Ampicillin PMID:16157277 The Plexin D1 fragment was amplified by RT-PCR using the following primer pair: CAGGAAATGAACGCACACC TGAGGGACACAGACAACTGC; For in vitro transcription, use the HindIII restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and SP6 promoter for antisense probe generation. Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin fragment contains bp# 5751-6406 of NM_026376.3 2026-08-15 01:15:25 0
pCRII-Topo Cux2 in situ probe
 
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RRID:Addgene_45623 Cux2 in situ probe Mus musculus Ampicillin PMID:16157277 The Cux2 fragment was amplified by RT-PCR using the following primer pair: TCAGTCAACAGCTCCATTCG GACAGCGAGAAAGTCCTTGG For in vitro transcription, use the HindIII restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and SP6 promoter for antisense probe generation. Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin fragment contains nt 1069-1694 on NM_007804 2026-08-15 01:15:25 0
pCRII-Topo Crim1 in situ probe
 
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RRID:Addgene_45600 Crim1 in situ probe Mus musculus Ampicillin PMID:15664173 Fragment was amplified using the following primers: TCTCAAGACTGTTGGTTGCTG TGAACAACCAATGATAGCACAG For in vitro transcription, use the NotI restriction digest and SP6 promoter for sense probe generation and the HindIII restriction digest and T7 promoter for antisense probe generation. Please note that Addgene's sequencing results match bp#4062-4502 of NM_015800.3, not bp# 4708-5148 as indicated on plasmid map. The plasmid functions as described in the associated publication. Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin insert contains bp#4062-4502 of NM_015800.3 2026-08-15 01:15:25 0
pCRII-Topo Diap3 in situ probe
 
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RRID:Addgene_45602 Diap3 in situ probe Mus musculus Ampicillin PMID:15664173 Fragment was amplified using the following primers: AGCAGTTCGCTGTTGTGATG GCACGTTTCTCCTTTTCTGC For in vitro transcription, use the SpeI restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and SP6 promoter for antisense probe generation. Please note that Addgene's sequencing results match bp# 1589-2321 of BC028920, not bp# 1610-2321 as indicated on plasmid map. The plasmid functions as described in the associated publication. Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin fragment contains bp# 1589-2321 of BC028920 2026-08-15 01:15:25 1
FLAG-VAMP7
 
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RRID:Addgene_45913 vesicle-associated membrane protein 7 Mus musculus Ampicillin PMID:23217709 Backbone Size:6400; Vector Backbone:CMV10 FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:27 1
pCRII-Topo Grp
 
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RRID:Addgene_45871 GRP in situ probe Mus musculus Ampicillin For in vitro transcription, use the NotI restriction digest and Sp6 promoter for sense probe generation and the HindIII restriction digest and T7 promoter for antisense probe generation. Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin nt 66-504 on NM_175012 2026-08-15 01:15:27 0
pCRII-Topo Inhba
 
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RRID:Addgene_45868 Inhba in situ probe Mus musculus Ampicillin PMID:19793993 The Inhba fragment was amplified by RT-PCR using the following primer pair: GCGATCAGAAAGCTTCATGTG AGACTGGCACCACTCTCCTG For in vitro transcription, use the Spe1 restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and Sp6 promoter for antisense probe generation. Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin nt 428-933 from Inhba mRNA (NM_008380.1) 2026-08-15 01:15:27 0
pEGFP eIF2beta C-term (aa 89-331)
 
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RRID:Addgene_45901 eIF2S2 aa89-331 Mus musculus Kanamycin PMID:11959995 PCR amplified using EcoRI/BamHI containing primers. G321D mutation is also present. Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin aa 89-331 2026-08-15 01:15:27 0

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