Plasmids   Select Another Resource Report Type

http://www.addgene.org/37119

Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:K. Deisseroth Lab; Backbone Size:5613; Vector Backbone:pAAV-Ef1a-DIO-hChR2(H134R)-mCherry-WPRE-pA; Vector Types:AAV, Cre/Lox, Cre-Off; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22866029

Proper citation: RRID:Addgene_37119 Copy   

•   Source: Addgene

http://www.addgene.org/37341

Species: Synthetic
Genetic Insert: NLS YFP
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6000; Vector Backbone:pQC; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21397594

Proper citation: RRID:Addgene_37341 Copy   

•   Source: Addgene

http://www.addgene.org/37343

Species: Synthetic
Genetic Insert: membrane Venus
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6000; Vector Backbone:pQC; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21397594

Proper citation: RRID:Addgene_37343 Copy   

•   Source: Addgene

http://www.addgene.org/37334

Species: Synthetic
Genetic Insert: NLS CFP
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6000; Vector Backbone:pQC; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21397594

Proper citation: RRID:Addgene_37334 Copy   

•   Source: Addgene

http://www.addgene.org/37336

Species: Synthetic
Genetic Insert: membrane CFP
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6000; Vector Backbone:pQC; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21397594

Proper citation: RRID:Addgene_37336 Copy   

•   Source: Addgene

http://www.addgene.org/37354

Species: Synthetic
Genetic Insert: NLS-mCherry
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6000; Vector Backbone:pQC; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21397594

Proper citation: RRID:Addgene_37354 Copy   

•   Source: Addgene

http://www.addgene.org/37351

Species: Synthetic
Genetic Insert: membrane TdTomato
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6000; Vector Backbone:pQC; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21397594
Comments: *palmitoylation sequence: 5'-atgctgtgctgtatgagaagaaccaaacaggttgaaaagaatgatgaggaccaaaagatc-3'

Proper citation: RRID:Addgene_37351 Copy   

•   Source: Addgene

http://www.addgene.org/44463

Species: Synthetic
Genetic Insert: QQR ZFN
Vector Backbone Description: Backbone Size:13812; Vector Backbone:pHEX2; Vector Types:Plant Transformation; Bacterial Resistance:Spectinomycin
Comments: QQR ZFN reference: Even-Faitelson L, Samach A, Melamed-Bessudo C, Avivi-Ragolsky N, Levy AA (2011) Localized egg-cell expression of effector proteins for targeted modification of the Arabidopsis genome. The Plant Journal 68 (5):929-937. doi:10.1111/j.1365-313X.2011.04741.x Please refer to the references provided below for matters relating to ownership of pHEX2 for anything other than academic use. pHEX2 reference: Hellens R, Allan A, Friel E, Bolitho K, Grafton K, Templeton M, Karunairetnam S, Gleave A, Laing W (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1 (1):13. doi:10.1186/1746-4811-1-13 pART27 (used to derive pHEX2) reference: Gleave AP (1992) A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20:1203-1207

Proper citation: RRID:Addgene_44463 Copy   

•   Source: Addgene

http://www.addgene.org/44464

Species: Synthetic
Genetic Insert: AtCRU3 T494 TALENs
Vector Backbone Description: Backbone Size:13812; Vector Backbone:pHEX2; Vector Types:Plant Transformation; Bacterial Resistance:Spectinomycin
Comments: pZHY013 reference: Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, Voytas DF (2012) TALENs enable efficient plant genome engineering. Plant Physiology. doi:10.1104/pp.112.205179 Please refer to the references provided below for matters relating to ownership of pHEX2 for anything other than academic use. pHEX2 reference: Hellens R, Allan A, Friel E, Bolitho K, Grafton K, Templeton M, Karunairetnam S, Gleave A, Laing W (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1 (1):13. doi:10.1186/1746-4811-1-13 pART27 (used to derive pHEX2) reference: Gleave AP (1992) A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20:1203-1207

Proper citation: RRID:Addgene_44464 Copy   

•   Source: Addgene

http://www.addgene.org/44441

Species: Synthetic
Genetic Insert: Horseradish peroxidase fused to the transmembrane domain of PDGFR
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23371551
Comments: This construct has the Ig k-chain leader sequence on the N-terminus, which directs the protein to the secretory pathway. HRP faces the extracellular side of the plasma membrane.

Proper citation: RRID:Addgene_44441 Copy   

•   Source: Addgene

http://www.addgene.org/44552

Species: Synthetic
Genetic Insert: PTETREG promoter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4456; Vector Backbone:pRS403; Vector Types:Yeast Expression, Synthetic Biology, Expression regulator/reporter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22511863
Comments: This plasmids was created as follows. First, the PTETREG promoter consisting of two tetO2 sites upstream of the minimal PCYC1 promoter was amplified from the pBB247 plasmid (Becskei, Séraphin, and Serrano, EMBO 2001, PMID: 11350942), and inserted into the pDN-G1GZmh plasmid between the AflII and BamHI sites instead of the PGAL1-D12 promoter resulting in the pDN-T2dGZmh plasmid. In order to facilitate the planned integration of the reporter plasmid into the his3Δ200 locus of the YPH500 strain, a small region bearing homology to the his3Δ200 locus was constructed by PCR and inserted in front of the HIS3 gene between the AhdI and AfeI sites of the pDN-T2dGZmh plasmid, resulting in the pDN-T2dGZmlh plasmid. Next, a second ADH1 terminator was removed from the pDN-T2dGZmlh plasmid, resulting in the final reporter pDN-T2dGZmxh plasmid bearing the yEGFP::zeoR fluorescent reporter gene under the control of the PTETREG rtTA-MF inducible promoter.

Proper citation: RRID:Addgene_44552 Copy   

•   Source: Addgene

http://www.addgene.org/44592

Species: Synthetic
Genetic Insert: Citrine-PTS1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10404; Vector Backbone:pMpGWB102; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23421791

Proper citation: RRID:Addgene_44592 Copy   

•   Source: Addgene

http://www.addgene.org/44586

Species: Synthetic
Genetic Insert: Citrine-NLS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3374; Vector Backbone:pDONR207; Vector Types:; Bacterial Resistance:Gentamicin
Defining Citation: PMID:23421791

Proper citation: RRID:Addgene_44586 Copy   

•   Source: Addgene

http://www.addgene.org/44719

Species: Synthetic
Genetic Insert: Cas9-2A-GFP
Vector Backbone Description: Backbone Size:4200; Vector Backbone:pCAG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23561441
Comments: For more information on Musunuru Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Musunuru/

Proper citation: RRID:Addgene_44719 Copy   

•   Source: Addgene

http://www.addgene.org/44795

Species: Synthetic
Genetic Insert: FREQ-Seq Barcode: TAGCCA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23118913
Comments: These plasmids are used to generate bridging primers with Illumina-compatible barcodes for allele frequency sequencing (FREQ-Seq). Similar to RNA-Seq, the FREQ-Seq method can rapidly determine allele frequencies directly from mixed populations with minimal library preparation.

Proper citation: RRID:Addgene_44795 Copy   

•   Source: Addgene

http://www.addgene.org/44794

Species: Synthetic
Genetic Insert: FREQ-Seq Barcode: GCTTAG
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23118913
Comments: These plasmids are used to generate bridging primers with Illumina-compatible barcodes for allele frequency sequencing (FREQ-Seq). Similar to RNA-Seq, the FREQ-Seq method can rapidly determine allele frequencies directly from mixed populations with minimal library preparation.

Proper citation: RRID:Addgene_44794 Copy   

•   Source: Addgene

http://www.addgene.org/44796

Species: Synthetic
Genetic Insert: FREQ-Seq Barcode: TAACTT
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23118913
Comments: These plasmids are used to generate bridging primers with Illumina-compatible barcodes for allele frequency sequencing (FREQ-Seq). Similar to RNA-Seq, the FREQ-Seq method can rapidly determine allele frequencies directly from mixed populations with minimal library preparation.

Proper citation: RRID:Addgene_44796 Copy   

•   Source: Addgene

http://www.addgene.org/44790

Species: Synthetic
Genetic Insert: FREQ-Seq Barcode: CAAATA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23118913
Comments: These plasmids are used to generate bridging primers with Illumina-compatible barcodes for allele frequency sequencing (FREQ-Seq). Similar to RNA-Seq, the FREQ-Seq method can rapidly determine allele frequencies directly from mixed populations with minimal library preparation.

Proper citation: RRID:Addgene_44790 Copy   

•   Source: Addgene

http://www.addgene.org/44781

Species: Synthetic
Genetic Insert: FREQ-Seq Barcode: CGATGC
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23118913
Comments: These plasmids are used to generate bridging primers with Illumina-compatible barcodes for allele frequency sequencing (FREQ-Seq). Similar to RNA-Seq, the FREQ-Seq method can rapidly determine allele frequencies directly from mixed populations with minimal library preparation.

Proper citation: RRID:Addgene_44781 Copy   

•   Source: Addgene

http://www.addgene.org/44784

Species: Synthetic
Genetic Insert: FREQ-Seq Barcode: TTAAGC
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23118913
Comments: These plasmids are used to generate bridging primers with Illumina-compatible barcodes for allele frequency sequencing (FREQ-Seq). Similar to RNA-Seq, the FREQ-Seq method can rapidly determine allele frequencies directly from mixed populations with minimal library preparation.

Proper citation: RRID:Addgene_44784 Copy   

•   Source: Addgene