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Species: Synthetic
Genetic Insert: ccdB toxin gene and SpCas9 target site (EGFP site 1 NGG PAM)
Vector Backbone Description: Vector Backbone:p11-LacY-wtx1 (Addgene Plasmid #69056); Vector Types:selection plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36203014
Proper citation: RRID:Addgene_181746 Copy
Species: Synthetic
Genetic Insert: human/zebrafish codon optimized SpCas9
Vector Backbone Description: Vector Backbone:BPK764 (Addgene Plasmid #65767); Vector Types:Bacterial Expression, in vitro transcription; T7 promoter; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36203014
Proper citation: RRID:Addgene_181745 Copy
Species: Synthetic
Genetic Insert: SuperNova (monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5604; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34762472
Comments: As descried above, SN was developed by Dr. Takeharu Nagai (Osaka University).
https://www.addgene.org/53234/
Proper citation: RRID:Addgene_181741 Copy
Species: Synthetic
Genetic Insert: nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36593413
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.01.07.475005v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_181791 Copy
Species: Synthetic
Genetic Insert: nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36593413
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.01.07.475005v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_181792 Copy
Species: Synthetic
Genetic Insert: 7xFOP
Vector Backbone Description: Backbone Size:6957; Vector Backbone:pC0037; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31296651
Comments: From:
Veeman MT, Slusarski DC, Kaykas A, Louie SH, Moon RT. Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements. Curr Biol. 2003 Apr 15;13(8):680-5. doi: 10.1016/s0960-9822(03)00240-9. PMID: 12699626.
Proper citation: RRID:Addgene_181955 Copy
Species: Synthetic
Genetic Insert: KanR, R6K origin of replication
Vector Backbone Description: Vector Backbone:pUC19, R6K origin of replication; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36593413
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.01.07.475005v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_181794 Copy
Species: Synthetic
Genetic Insert: Rep2 and Ref2
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35281741
Proper citation: RRID:Addgene_182055 Copy
Species: Synthetic
Genetic Insert: Rep1 and Ref1
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35281741
Proper citation: RRID:Addgene_182054 Copy
Species: Synthetic
Genetic Insert: Rep6 and Ref6
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35281741
Proper citation: RRID:Addgene_182059 Copy
Species: Synthetic
Genetic Insert: Alb_A277c_attB pegRNA
Vector Backbone Description: Vector Backbone:pU6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34887556
Comments: The parent vector used for cloning pegRNA constructs via Golden Gate has been deposited to Addgene previously. The name of the parent vector is pU6-pegRNA-GG-acceptor and the Addgene number is 132777.
Proper citation: RRID:Addgene_182145 Copy
Species: Synthetic
Genetic Insert: attP-GA-EGFP-EF1α-PuroR-T2A-TagBFP
Vector Backbone Description: Vector Backbone:pEF1α; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34887556
Proper citation: RRID:Addgene_182140 Copy
Species: Synthetic
Genetic Insert: IDS2-A4b-attP-fwd pegRNA
Vector Backbone Description: Vector Backbone:pU6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34887556
Comments: The parent vector used for cloning pegRNA constructs via Golden Gate has been deposited to Addgene previously. The name of the parent vector is pU6-pegRNA-GG-acceptor and the Addgene number is 132777.
Proper citation: RRID:Addgene_182149 Copy
Species: Synthetic
Genetic Insert: CCR5-B584b-attB pegRNA
Vector Backbone Description: Vector Backbone:pU6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34887556
Comments: The parent vector used for cloning pegRNA constructs via Golden Gate has been deposited to Addgene previously. The name of the parent vector is pU6-pegRNA-GG-acceptor and the Addgene number is 132777.
Proper citation: RRID:Addgene_182148 Copy
Species: Synthetic
Genetic Insert: IDS2-B7b-attP-fwd pegRNA
Vector Backbone Description: Vector Backbone:pU6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34887556
Comments: The parent vector used for cloning pegRNA constructs via Golden Gate has been deposited to Addgene previously. The name of the parent vector is pU6-pegRNA-GG-acceptor and the Addgene number is 132777.
Proper citation: RRID:Addgene_182150 Copy
Species: Synthetic
Genetic Insert: MiniE-DBCO
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32786411
Proper citation: RRID:Addgene_181985 Copy
Species: Synthetic
Genetic Insert: attP-EGFP-EF1α-PuroR-T2A-TagBFP
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34887556
Proper citation: RRID:Addgene_182139 Copy
Species: Synthetic
Genetic Insert: cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36303019
Comments: Please visit https://doi.org/10.1101/2021.08.27.457999 for bioRxiv preprint.
Proper citation: RRID:Addgene_182282 Copy
Species: Synthetic
Genetic Insert: cAMPFIRE-M (cAMP sensor)
Vector Backbone Description: Backbone Size:5922; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36303019
Comments: Please visit https://doi.org/10.1101/2021.08.27.457999 for bioRxiv preprint.
Proper citation: RRID:Addgene_182284 Copy
Species: Synthetic
Genetic Insert: cAMPFIRE-L (cAMP sensor)
Vector Backbone Description: Backbone Size:5922; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36303019
Comments: Please visit https://doi.org/10.1101/2021.08.27.457999 for bioRxiv preprint.
Proper citation: RRID:Addgene_182283 Copy
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