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Species: Homo sapiens
Genetic Insert: CARD8
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34019797
Proper citation: RRID:Addgene_169968 Copy
Species: Homo sapiens
Genetic Insert: CARD8
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34019797
Proper citation: RRID:Addgene_169981 Copy
Species: Homo sapiens
Genetic Insert: CARD8
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34019797
Proper citation: RRID:Addgene_169975 Copy
Species: Homo sapiens
Genetic Insert: CARD8
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34019797
Proper citation: RRID:Addgene_169971 Copy
Species: Synthetic
Genetic Insert: bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
Vector Backbone Description: Backbone Size:5238; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33927418
Comments: This is a bacterial codon-optimized SpCas9 (H840A) sequence within pET-28b backbone (Addgene #73018) fused to bipartite NLS, a 33-amino acid linker and the engineered M-MLV RT from the parental pCMV-PE2 plasmid (Addgene #132775). To this construct, a short GS linker and a 6xHis-tag are added to C terminus.
Proper citation: RRID:Addgene_170103 Copy
Genetic Insert: T7-RNA polymerase
Vector Backbone Description: Backbone Size:3262; Vector Backbone:pET28a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34985758
Proper citation: RRID:Addgene_170101 Copy
Species: Homo sapiens
Genetic Insert: ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35044296
Proper citation: RRID:Addgene_170136 Copy
Species: E. coli
Genetic Insert: E. coli type I-E Cas5
Vector Backbone Description: Backbone Size:5221; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34985758
Comments: If used in a publication, please acknowledge that the plasmid pEcCas5 was generated by U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility. The associated work was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.
Proper citation: RRID:Addgene_170090 Copy
Species: Mus musculus
Genetic Insert: nCas9(H840A)-M-MLV
Vector Backbone Description: Backbone Marker:Qi-Jun Chen; Vector Backbone:pHUE411 (modified from pCambia); Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33767395
Proper citation: RRID:Addgene_170131 Copy
Species: E. coli
Genetic Insert: E. coli type I-E Cse2
Vector Backbone Description: Backbone Size:5230; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34985758
Comments: If used in a publication, please acknowledge that the plasmid pEcCse2 was generated by U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility. The associated work was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.
Proper citation: RRID:Addgene_170094 Copy
Species: Homo sapiens
Genetic Insert: Circular 200,100 guide RNA with 8bp bulge loops
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35145312
Proper citation: RRID:Addgene_170121 Copy
Genetic Insert: RESCUE-DDC-λN-NES
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35044296
Proper citation: RRID:Addgene_170154 Copy
Species: Homo sapiens
Genetic Insert: BoxB-RAB7A(ACG)-MS2
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35044296
Proper citation: RRID:Addgene_170155 Copy
Species: Homo sapiens
Genetic Insert: NES-MCP-RESCUE-DDN
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35044296
Proper citation: RRID:Addgene_170153 Copy
Species: Homo sapiens
Genetic Insert: EGFP-RSKL
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_170165 Copy
Species: Homo sapiens
Genetic Insert: EGFP-SKL
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_170166 Copy
Species: Bradyrhizobium sp. (strain ORS 278)
Genetic Insert: NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
Vector Backbone Description: Backbone Marker:Originally derived from pEGFP-N1 backbone after several modifications in our lab.; Backbone Size:3427; Vector Backbone:pQP-Select; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34162879
Comments: To design iLight, a full-length bacterial phytochrome IsPadC from Idiomarina sp. A28L was used as an original template. First, diguanylyl cyclase effector in IsPadC was removed, then following mutations were introduced: I68F, H80Q, A86T, R90S S242C, E274K, R295H I360V, and L464V. After that, iLight was human codon optimized for mammalian expression.
Proper citation: RRID:Addgene_170273 Copy
Species: Mus musculus
Genetic Insert: nuclear export factor 1, CAST allele
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7002; Vector Backbone:Modified pGBKT7; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_170267 Copy
Species: Synthetic
Genetic Insert: momeric Cherry
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7002; Vector Backbone:Modified pGBKT7; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_170264 Copy
Species: Synthetic
Genetic Insert: momeric Grape 3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7002; Vector Backbone:Modified pGBKT7; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_170265 Copy
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