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Species: Other
Genetic Insert: Met-tRNA formyltransferase
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111492 Copy
Species: Other
Genetic Insert: Gly-tRNA synthetase A
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111496 Copy
Species: Other
Genetic Insert: Ser-tRNA synthetase
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111487 Copy
Species: Other
Genetic Insert: Phe-tRNA synthetase A
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111488 Copy
Species: Other
Genetic Insert: Pro-tRNA synthetase
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111486 Copy
Species: Other
Genetic Insert: translational elongation factor G
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29131146
Comments: Gene comes from E.coli.
Proper citation: RRID:Addgene_111489 Copy
Species: Other
Genetic Insert: APEX2
Vector Backbone Description: Vector Backbone:pMV306; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29708735
Comments: This plasmid is for theophylline-inducible expression of APEX2 in the cytoplasm of mycobacteria. It is a single-copy integrating vector in mycobacteria. The APEX gene was subcloned from pcDNA3-mito-APEX and subsequently mutated by site-directed mutagenesis to generate the optimized variant APEX2.
See also references: Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science. 2013;339(6125):1328-31. PMCID: 3916822.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat Methods. 2015;12(1):51-4. PMCID: 4296904
Proper citation: RRID:Addgene_111700 Copy
Species: Other
Genetic Insert: APEX2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29708735
Comments: This plasmid is for IPTG-inducible expression of APEX2 in the E. coli cytoplasm. The APEX gene was subcloned from pcDNA3-mito-APEX and subsequently mutated by site-directed mutagenesis to generate the optimized variant APEX2.
See also references: Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science. 2013;339(6125):1328-31. PMCID: 3916822.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat Methods. 2015;12(1):51-4. PMCID: 4296904
Proper citation: RRID:Addgene_111702 Copy
Species: Other
Genetic Insert: APEX2
Vector Backbone Description: Vector Backbone:pRibo BsaHind; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29708735
Comments: This plasmid is for theophylline-inducible expression of APEX2 in the cytoplasm of mycobacteria. It is episomal and multi-copy. The APEX gene was subcloned from pcDNA3-mito-APEX and subsequently mutated by site-directed mutagenesis to generate the optimized variant APEX2.
See also references: Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science. 2013;339(6125):1328-31. PMCID: 3916822.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat Methods. 2015;12(1):51-4. PMCID: 4296904
Proper citation: RRID:Addgene_111698 Copy
Species: Other
Genetic Insert: APEX2
Vector Backbone Description: Vector Backbone:pRibo BsaHind; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29708735
Comments: This plasmid is for theophylline-inducible expression of APEX2 in the periplasm of mycobacteria. It is episomal and multi-copy. The APEX gene was subcloned from pcDNA3-mito-APEX and subsequently mutated by site-directed mutagenesis to generate the optimized variant APEX2.
See also references: Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science. 2013;339(6125):1328-31. PMCID: 3916822.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat Methods. 2015;12(1):51-4. PMCID: 4296904
Proper citation: RRID:Addgene_111699 Copy
Species: Other
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:Unknown; Backbone Size:4000; Vector Backbone:Synthetic; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30460568
Proper citation: RRID:Addgene_112027 Copy
Species: Other
Genetic Insert: TelN
Vector Backbone Description: Vector Backbone:pAA.3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29724956
Proper citation: RRID:Addgene_112011 Copy
Species: Other
Genetic Insert: AtDeadCAS9 D10A H840A without stop codon
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112073 Copy
Species: Other
Genetic Insert: 3xmVenus
Vector Backbone Description: Vector Backbone:pAGM1301; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112074 Copy
Species: Other
Genetic Insert: AtCAS9 D10A
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112078 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pICH41258; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112080 Copy
Species: Other
Genetic Insert: TagRFP
Vector Backbone Description: Vector Backbone:pICH41258; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112081 Copy
Species: Other
Genetic Insert: AtCAS9
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis, however the amino acid sequence is as in the original AtCas9 pDe-CAS9 vector from H. Puchta lab.
Proper citation: RRID:Addgene_112070 Copy
Species: Other
Genetic Insert: iGABASnFR.F102Y.Y137L
Vector Backbone Description: Vector Backbone:pAAV.SynFLEX; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31308547
Comments: Please visit https://www.biorxiv.org/content/early/2018/05/14/322578 for bioRxiv preprint.
Proper citation: RRID:Addgene_112171 Copy
Species: Other
Genetic Insert: iGABASnFR.R205A
Vector Backbone Description: Vector Backbone:pAAV.hSynapsin; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31308547
Comments: Please visit https://www.biorxiv.org/content/early/2018/05/14/322578 for bioRxiv preprint.
Proper citation: RRID:Addgene_112162 Copy
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