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Species: Caenorhabditis elegans
Genetic Insert: abu-1 promoter and coding region
Vector Backbone Description: Backbone Marker:Available at Addgene (#1494); Backbone Size:4487; Vector Backbone:pPD95_75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12186849
Comments: Addgene sequencing results show there is a S408P substitution in amino acid sequence in the coding region.
abu-1::abu-1::gfp C. elegans reporter gene. The promoter and coding region of abu-1 was ligated in frame with GFP to produce abu1::abu-1::gfp
Proper citation: RRID:Addgene_21825 Copy
Species: Caenorhabditis elegans
Genetic Insert: Otub1 ce
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4751; Vector Backbone:pProEX HTa; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22367539
Proper citation: RRID:Addgene_37196 Copy
Species: Caenorhabditis elegans
Genetic Insert: npp-16
Vector Backbone Description: Backbone Marker:Caldwell(?); Backbone Size:2790; Vector Backbone:pL4440gw; Vector Types:Worm Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20335358
Proper citation: RRID:Addgene_37495 Copy
Species: Caenorhabditis elegans
Genetic Insert: FBF-2::fbf-2 3’UTR
Vector Backbone Description: Backbone Marker:custom; Backbone Size:6700; Vector Backbone:pEV1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20335358
Proper citation: RRID:Addgene_37498 Copy
Species: Caenorhabditis elegans
Genetic Insert: rap-1::mGFP
Vector Backbone Description: Vector Backbone:pCR8/GW/TOPO; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:40748210
Proper citation: RRID:Addgene_245765 Copy
Species: Caenorhabditis elegans
Genetic Insert: rap-1::mCherry
Vector Backbone Description: Vector Backbone:pCR8/GW/TOPO; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:40748210
Proper citation: RRID:Addgene_245764 Copy
Species: Caenorhabditis elegans
Genetic Insert: tiam-1::mGFP
Vector Backbone Description: Vector Backbone:pDEST; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40748210
Proper citation: RRID:Addgene_245769 Copy
Species: Caenorhabditis elegans
Genetic Insert: rap-1::mGFP
Vector Backbone Description: Vector Backbone:pDEST; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40748210
Proper citation: RRID:Addgene_245768 Copy
Species: Caenorhabditis elegans
Genetic Insert: rap-1(G12V)::mGFP
Vector Backbone Description: Vector Backbone:pCR8/GW/TOPO; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:40748210
Proper citation: RRID:Addgene_245772 Copy
Species: Caenorhabditis elegans
Genetic Insert: rac-2::mGFP
Vector Backbone Description: Vector Backbone:pDEST; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40748210
Proper citation: RRID:Addgene_245776 Copy
Species: Caenorhabditis elegans
Genetic Insert: rac-2::mGFP
Vector Backbone Description: Vector Backbone:pCR8/GW/TOPO; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:40748210
Proper citation: RRID:Addgene_245773 Copy
Species: Caenorhabditis elegans
Genetic Insert: cyp-33e1
Vector Backbone Description: Backbone Size:4134; Vector Backbone:pCS2+8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39504959
Proper citation: RRID:Addgene_227156 Copy
Species: Caenorhabditis elegans
Genetic Insert: cyp-33e1
Vector Backbone Description: Backbone Size:4134; Vector Backbone:pCS2+8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39504959
Proper citation: RRID:Addgene_227157 Copy
Species: Caenorhabditis elegans
Genetic Insert: cyp-33e1
Vector Backbone Description: Backbone Size:4134; Vector Backbone:pCS2+8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39504959
Proper citation: RRID:Addgene_227151 Copy
Species: Caenorhabditis elegans
Genetic Insert: IDH-1
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pTXB3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40943163
Proper citation: RRID:Addgene_224142 Copy
Species: Caenorhabditis elegans
Genetic Insert: cGAL[cGAL(DBD)-linker-QFAD]-tbb-3'UTR
Vector Backbone Description: Vector Backbone:pNM3469; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39841730
Comments: The E. coli strain DH5α bacterial clone with this construct grows slowly, resulting in noticeably small numbers of colonies following overnight incubation. The DNA construct is likely toxic to the E. coli strain DH5α, resulting in unexpected random insertion or deletion. If this plasmid is transformed into DH5α competent cells. It is advisable to routinely sequence of the section of cGAL(DBD)-linker-QFAD-tbb-2 3'UTR following each fresh miniprep from a new bacterial culture to confirm absence of any mutations. To overcome this issue, the deposited bacterial stock of this plasmid is with NEB® Turbo competent E. coli, which tolerates this construct better and produces the plasmid with the correct sequence.
Proper citation: RRID:Addgene_229867 Copy
Species: Caenorhabditis elegans
Genetic Insert: EFF-1
Vector Backbone Description: Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35794124
Proper citation: RRID:Addgene_240504 Copy
Species: Caenorhabditis elegans
Genetic Insert: pSKI
Vector Backbone Description: Backbone Size:2598; Vector Backbone:pPD95_75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40973646
Comments: Please visit https://doi.org/10.1101/2024.12.06.627295 for bioRxiv preprint.
Proper citation: RRID:Addgene_232484 Copy
Species: Caenorhabditis elegans
Genetic Insert: mir50-5
Vector Backbone Description: Backbone Marker:Bartel lab (Addgene plasmid# 46646); Backbone Size:8038; Vector Backbone:pcDNA3.2 V5-DEST mir-1-1 reporter; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23415231
Proper citation: RRID:Addgene_46714 Copy
Species: Caenorhabditis elegans
Genetic Insert: mir50-3
Vector Backbone Description: Backbone Marker:Bartel lab (Addgene plasmid# 46646); Backbone Size:8038; Vector Backbone:pcDNA3.2 V5-DEST mir-1-1 reporter; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23415231
Proper citation: RRID:Addgene_46712 Copy
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