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Species: Rattus norvegicus
Genetic Insert: PSD-95-mCherry
Vector Backbone Description: Backbone Size:9500; Vector Backbone:FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26853302
Comments: This plasmid can be used with the low-efficiency Cre in Plasmid 101242: pSin wPGK-Cre ( https://www.addgene.org/101242/ ).
Proper citation: RRID:Addgene_73919 Copy
Species: Rattus norvegicus
Genetic Insert: Teal-gephyrin
Vector Backbone Description: Vector Backbone:FU-dioYFP-W; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26853302
Comments: This plasmid can be used with the low-efficiency Cre in Plasmid 101242: pSin wPGK-Cre ( https://www.addgene.org/101242/ ).
Proper citation: RRID:Addgene_73918 Copy
Species: Rattus norvegicus
Genetic Insert: Psd95 shRNA
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16815335
Comments: Map: FHUG+W-HinDIII-SalI-XbaI-(p95b)-KpnI-SacII-PspOMI-SmaI-BamHI-(GFP)-BglII-BsrBI-EcoRV-HinDIII-ClaI
Also associated with Liu, et al. J Neurophysiol. 2014 Feb;111(3):648-58. doi: 10.1152/jn.00262.2013 PMID 24225540.
Psd95 shRNA expressed under control of H1 promoter. Psd95 coding sequence modified to GGATATTCAAGCGCATAAG make it resistant to the shRNA. Psd95-EGFP fusion protein expressed under control of UbC promoter.
The publication by Schluter et al linked above describes the creation of the beta isoform of Psd95 in detail
Proper citation: RRID:Addgene_74014 Copy
Species: Rattus norvegicus
Genetic Insert: SAP97 beta isoform
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16815335
Comments: Map: FUGW-SalI-XbaI-BamHI-(GFP+)-BspEI-BglII-XhoI-SacI-HinDIII-BstBI-EcoRI-(p97 I3)-EcoRI-EcoRV-HinDIII-ClaI-FUGW
Proper citation: RRID:Addgene_74018 Copy
Species: Rattus norvegicus
Genetic Insert: Psd95
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16815335
Comments: Map: FUGW-HinDIII-SalI-XbaI-BamHI-SmaI-KpnI-EcoRI-XbaI-(p95)
PSD95 is isoform alpha.
Proper citation: RRID:Addgene_74016 Copy
Species: Rattus norvegicus
Genetic Insert: Psd95
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18215622
Comments: Map: FHUGW-(pUb)-HinDIII-SalI-XbaI-(P95PDZ)-BsrGI (in EGFP)-NheI-XhoI-EcoRV-HinDIII-ClaI
Proper citation: RRID:Addgene_74022 Copy
Species: Rattus norvegicus
Genetic Insert: Psd95
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18215622
Comments: Map: FUGW-HinDIII-SalI-XbaI-BamHI-SmaI-KpnI-EcoRI-XbaI-(P95E17R)
Proper citation: RRID:Addgene_74029 Copy
Species: Rattus norvegicus
Genetic Insert: Psd95
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18215622
Comments: Map: FUGW-HinDIII-SalI-XbaI-BamHI-SmaI-KpnI-EcoRI-XbaI-(P95Q15A)
Proper citation: RRID:Addgene_74028 Copy
Species: Rattus norvegicus
Genetic Insert: Psd95
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9941; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19081375
Comments: Addgene did not confirm the presence of the S73D mutation.
Map: FHUGW-(pUb)-HinDIII-SalI-XbaI-(p95S73D)
Proper citation: RRID:Addgene_74040 Copy
Species: Rattus norvegicus
Genetic Insert: Rem2
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21485012
Comments: Please note that the sequence of the myc epitope tag in this plasmid is MQKLISEEDLL, which differs from the standard EQKLISEEDLL.
Proper citation: RRID:Addgene_51596 Copy
Species: Rattus norvegicus
Genetic Insert: shRNA2
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSuper; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24036351
Proper citation: RRID:Addgene_51598 Copy
Species: Rattus norvegicus
Genetic Insert: alpha MHC
Vector Backbone Description: Vector Backbone:pGL3-Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_51934 Copy
Species: Rattus norvegicus
Genetic Insert: MIBP1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22294689
Proper citation: RRID:Addgene_51871 Copy
Species: Rattus norvegicus
Genetic Insert: MIBP1
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11872163
Comments: Makino, R., Akiyama, K., Yasuda, J., Mashiyama, S., Honda, S., Sekiya, T., & Hayashi, K. (1994). Cloning and characterization of a c-myc intron binding protein (MIBP1). Nucleic acids research, 22(25), 5679-5685.
Proper citation: RRID:Addgene_51873 Copy
Species: Rattus norvegicus
Genetic Insert: CLC3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10915634
Comments: Insert represents a portion of the 5’UTR and the full open reading frame of ClC-3 beginning at nucleotide 251 according to GenBank Accession number D17521.
Proper citation: RRID:Addgene_52422 Copy
Species: Rattus norvegicus
Genetic Insert: PSD-95
Vector Backbone Description: Backbone Marker:Evrogen; Backbone Size:4733; Vector Backbone:pTagRFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: HindIII and EcoRI were used to cut PSD-95 from PSD-95-pGW1 (received from David Bredt) and ligate it into pTagRFP-N.
Proper citation: RRID:Addgene_52671 Copy
Species: Rattus norvegicus
Genetic Insert: ACTN3
Vector Backbone Description: Backbone Marker:Hell lab, Addgene plasmid #52675; Vector Backbone:pSIL-EGFP; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19028690
Comments: EGFP is a transfection marker, not a fusion.
RNAi targeted at 455–473 from the start sequence of rat α-actinin-3 (the sense chain was 5’-aacgagaagctgatggaggttcaagagacctccatcagcttctcgtttttttt-3’. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3’ end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer™ vector for expression of the respective sequences under the U6 promotor.
Proper citation: RRID:Addgene_52678 Copy
Species: Rattus norvegicus
Genetic Insert: SNAP-25B
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23536066
Proper citation: RRID:Addgene_53235 Copy
Species: Rattus norvegicus
Genetic Insert: VAMP2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23536066
Proper citation: RRID:Addgene_53237 Copy
Species: Rattus norvegicus
Genetic Insert: VAMP2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23536066
Proper citation: RRID:Addgene_53238 Copy
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