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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pGL3-Tet1 promoter A Resource Report Resource Website 1+ mentions |
RRID:Addgene_63881 | Tet1 promoter fragment A | Mus musculus | Ampicillin | PMID:25582196 | Backbone Size:4807; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:06 | 1 | ||
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pGL3-Tet1 promoter B Resource Report Resource Website |
RRID:Addgene_63882 | Tet1 promoter fragment B | Mus musculus | Ampicillin | PMID:25582196 | Backbone Size:4807; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:06 | 0 | ||
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pGL3-SV40P-Tet1 enhancer E Resource Report Resource Website |
RRID:Addgene_63880 | Tet1 enhancer fragment E | Mus musculus | Ampicillin | PMID:25582196 | Backbone Size:5005; Vector Backbone:pGL3-Promoter; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:06 | 0 | ||
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pST50Trc1-HISDHFR Resource Report Resource Website 1+ mentions |
RRID:Addgene_63935 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using XbaI/BamHI | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 1 | |||
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pST50Trc1-STRDHFR Resource Report Resource Website |
RRID:Addgene_63937 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using XbaI/BamHI | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 | |||
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pST50Trc1-FLAGDHFR Resource Report Resource Website |
RRID:Addgene_63938 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using XbaI/BamHI | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 | |||
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Luc-Nanog-3'UTR Resource Report Resource Website 1+ mentions |
RRID:Addgene_63893 | Nanog homeobox | Mus musculus | Ampicillin | PMID:22020437 | Addgene's quality control sequence with EBV-rev primer shows a single bp mismatch with the available reference sequence. | Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-Control; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 1 | |
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Luc-Nanog-3'UTRmut Resource Report Resource Website |
RRID:Addgene_63894 | Nanog homeobox | Mus musculus | Ampicillin | PMID:22020437 | Mir-34a binding site was mutated to remove the seed region | Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-Control; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:06 | 0 | |
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pComb3XSS Resource Report Resource Website 10+ mentions |
RRID:Addgene_63890 | SS Stuffer | Synthetic | Ampicillin | PMID:10986398 | pComb3X is the newest of the pComb vectors. Improvements over pComb3 include increased stability and introduction of an asymmetric SfiI cassette for directional cloning of full Fab, scFv, peptide and other protein for phage display. 6xHis and HA tags allow for purification and detection. An amber stop codon was introduced to turn-off expression of the pIII fusion protein by switching to a non-supressor strain of E. coli allowing production of soluble protein without subcloning. Alternatively, the gene for phage protein pIII can be removed by SpeI/NheI digest. pComb3XSS is recommended for preparation of vector for library cloning. The “SS” refers to the double stuffer, a 1200bp stuffer in the Fab light chain cloning region bounded by SacI and XbaI restriction sites and a 300bp stuffer in Fab heavy chain cloning region bound by XhoI and SpeI restriction sites. Also, the 1600bp double stuffer (both stuffers plus the leader sequence between the Fab light chain and heavy chain cloning regions) can be removed by SfiI digest so that non-Fab genes of interest can be cloned. Also available on Addgene: pComb3XTT and pComb3XLambda are only needed at templates for the construction of chimeric Fab libraries as described in Phage Display: A Laboratory Manual. pComb3XTT can also be used as an Fab expression control. Select References: Barbas, C. F., III; Burton, D. R.; Scott, J.K., Silverman, G.J. Eds. (2001) Phage Display: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York Rader, C; Popkov, M.; Neves, J.A.; Barbas III, C.F. (2002) Integrin _v_3 Targeted Therapy of Kaposi’s Sarcoma with an In Vitro Evolved Antibody. FASEB, 16(14):2000-2. Berry, J.D.; Rutherford, J.; Silverman, G.J.; Kaul, R.; Elia, M.; Gobuty, S.; Fuller, R.; Plummer, F.A.; & Barbas III, C.F. (2003) Development of Functional Human Monoclonal Single-Chain Variable Fragment Antibody Against HIV-1 From Human Cervical B cells. Hybridoma and Hybridomics, 22(2):, 97-108. Jendreyko N, Popkov M, Beerli RR, Chung J, McGavern DB, Rader C, Barbas CF 3rd. (2003) Intra-diabodies: Bispecific, tetravalent antibodies for the simultaneous functional knockout of two cell surface receptors. J Biol Chem., 278(48):, 47812-9. Steinberger, P.; Sutton, J.K.; Rader, C.; Elia, M.; and Barbas III, C. F. (2000) Generation and Characterization of a Recombinant Human CCR5-specific Antibody: A Phage Display Approach for Rabbit Antibody Humanization. J. Biol. Chem., 275,, 36073-36078. Goncalves, J.; Kilva, F.; Freitas-Vieira, A.; Santa-Marta, M.; Malho, R.; Yang, X.; Gabuzda, D.; and Barbas III, C.F. (2002) Functional Neutralization of HIV-1 Vif Protein by Intracellular Immunization Inhibits Reverse Transcription and Viral Replication. J. Biol. Chem., 277(35):32036-45. Popkov, M.; Mage, R.G.; Alexander, C.B.; Thundivalappil, S.; Barbas III, C.F.; Rader, C. (2003) Rabbit immune repertoires as sources for therapeutic monoclonal antibodies: The impact of Kappa Allotype-correlated variation in cysteine content on antibody libraries selected by phage display. J. Mol. Biol., 325:325-335. Chung, J.; Rader, C.; Popkov, M.; Hur, Y.-M.; Kim, H.-K.; Lee, Y.-J.; & Barbas III, C. F. (2004) Integrin _IIb_3 specific synthetic human monoclonal antibodies and HCDR3 peptides that potently inhibit platelet aggregation,FASEB 18(2):361-3. Popkov, M.; Jendreyko, N.; Gonzalez-Sapienza, G.; Mage, R.G.; Rader, C.; Barbas III, C.F. (2004) Human/mouse cross-reactive anti-VEGF receptor 2 recombinant antibodies selected from animmune b9 allotype rabbit antibody library, J. Immunol. Methods, 288(1-2):149-164. Popkov, M.; Rader, C.; Barbas III, C.F. (2004) Isolation of human prostate cancer reactive antibodies using phage display technology, J. Immunol. Methods, 291:137-151. Tanaka, F.; Fuller, R.; Barbas III, C.F. (2005) Development of Small Designer Aldolase Enzymes: Catalytic Activity, Folding, and Substrate Specificity. Biochemistry, 44:7583-7592. This plasmid has been found to be prone to multimerization. Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. See our article on Plasmid Dimers and Multimers for more information (https://blog.addgene.org/plasmids-101-dimers-and-multimers) | Backbone Size:3300; Vector Backbone:pComb3H; Vector Types:Bacterial Expression, phage display; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 18 | |
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pST50Trc1-HPCNDHFR Resource Report Resource Website |
RRID:Addgene_63944 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using XbaI/BamHI | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 | |||
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pST50Trc1-FLAGNDHFR Resource Report Resource Website |
RRID:Addgene_63943 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using XbaI/BamHI | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 | |||
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pST50Trc1-HPCHISNDHFR Resource Report Resource Website |
RRID:Addgene_63948 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using XbaI/BamHI | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 | |||
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pST50Trc1-DHFRHIS Resource Report Resource Website |
RRID:Addgene_63949 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using XbaI/BamHI | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 | |||
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pST50Trc2-STRDHFR Resource Report Resource Website |
RRID:Addgene_63955 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using EcoRI/HindIII | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 | |||
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pST50Trc2-HISNDHFR Resource Report Resource Website |
RRID:Addgene_63958 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using EcoRI/HindIII | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 | |||
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pST50Trc1-DHFRCYD Resource Report Resource Website |
RRID:Addgene_63952 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using XbaI/BamHI | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 | |||
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pST50Trc1-DHFRSTR Resource Report Resource Website |
RRID:Addgene_63950 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using XbaI/BamHI | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 | |||
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pST50Trc2-DHFRFLAG Resource Report Resource Website |
RRID:Addgene_63969 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using EcoRI/HindIII | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 | |||
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pST50Trc2-FLAGHISNDHFR Resource Report Resource Website |
RRID:Addgene_63965 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using EcoRI/HindIII | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 | |||
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pST50Trc3-HPCDHFR Resource Report Resource Website |
RRID:Addgene_63975 | Ampicillin | PMID:15766881 | Replace DHFR with GOI using BamHI/NgoMIV; Clone out cassette using SacI/KpnI | Vector Backbone:pST50Trc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 0 |
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