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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pMM586
 
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RRID:Addgene_133592 dCas9 Saccharomyces cerevisiae Chloramphenicol PMID:31719930 Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:05:37 0
pMM582
 
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RRID:Addgene_133590 Linus Saccharomyces cerevisiae Chloramphenicol PMID:31719930 Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:05:37 0
pNW-sfGFP(SPS6)
 
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RRID:Addgene_133814 sfGFP(SPS6) Other Chloramphenicol PMID:29195004 Backbone Marker:Frenzel etal. 2018; Vector Backbone:pNW-Ppta-3TER; Vector Types:Bacterial Expression, E.coli/Bacillus spp. shuttle vector; Bacterial Resistance:Chloramphenicol 2026-08-15 01:05:40 0
pAD-sfGFP(Sp)-Pman
 
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1+ mentions
RRID:Addgene_133833 sfGFP(Sp) Synthetic Chloramphenicol PMID:29195004 Vector Backbone:pAD43-25; Vector Types:Bacterial Expression, E.coli/Bacillus spp. shuttle vector; Bacterial Resistance:Chloramphenicol 2026-08-15 01:05:39 1
pAD-mKate(KPS12)
 
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RRID:Addgene_133831 mKate(KPS12) Synthetic Chloramphenicol PMID:29195004 Backbone Marker:Dunn and Handelsman, 1999; Vector Backbone:pAD43-25; Vector Types:Bacterial Expression, E.coli/Bacillus spp. shuttle vector; Bacterial Resistance:Chloramphenicol 2026-08-15 01:05:39 0
pAD-SPS6-Pman
 
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RRID:Addgene_133906 sfGFP(SPS6) Synthetic Chloramphenicol PMID:29195004 Backbone Marker:Dunn and Handelsman, 1999; Vector Backbone:pAD43-25; Vector Types:Bacterial Expression, E.coli/Bacillus spp. shuttle vector; Bacterial Resistance:Chloramphenicol 2026-08-15 01:05:40 0
pBAC-lacZ
 
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1+ mentions
RRID:Addgene_13422 Chloramphenicol PMID:17406455 The complete assembled sequence for this plasmid may not be accurate. Addgene suggests sequence verifying the construct before starting your experiments. Bacterial cell-based two-hybrid (B2H) reporter vector. Use 12.5 ug/ml chloramphenicol for growth. The pBAC-lacZ plasmid is a mini-F' that can be replicated in standard E. coli strains, but because it is maintained as a single copy episome, it gives low DNA yields. pBAC-lacZ also contains a second, higher copy number origin of replication (oriV) that is only active in the presence of a trans-acting factor encoded by the trfA gene. Transformax EPI300 cells express trfA from an inducible promoter that we have found is inducible with arabinose. When the trfA gene is induced in Transformax EPI300 cells, the copy number of pBAC-lacZ plasmid is increased in the cells and reasonable yields of plasmid can be obtained using a standard miniprep procedure. Further notes on propagating this plasmid: grow an overnight culture (without arabinose) and then the next morning subculture 1:10 into medium that has a final concentration of arabinose of 1 mM. You then grow this culture for 5 to 6 hours at 37 C and then harvest the DNA. Backbone Size:11151; Vector Backbone:pBAC-lacZ; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol 2026-08-15 01:05:42 5
pSiMPlc_N + pSiMPlc_C
 
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RRID:Addgene_134312 cmR_gp41-1 N-intein + cmR_gp41-1 C-intein Synthetic Chloramphenicol PMID:31672972 pSiMPlc_N should be cotransformed with pSiMPlc_C (or vice versa) in E. Coli to get a reconstituted functional CAT thereby conferring resistance against chloramphenicol. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252). Please see the Sequences link for the individual plasmid sequences. The sample from Addgene will have both the plasmids pSiMPlc_N + pSiMPlc_C in it for the interested antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones. Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:05:44 0
pMP190
 
Resource Report
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RRID:Addgene_185922 Chloramphenicol PMID:24276795 The plasmid also confers streptomycin resistance. Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Spaink; Backbone Size:15000; Vector Backbone:pMP184; Vector Types:Bacterial Expression, IncQ; Bacterial Resistance:Chloramphenicol 2026-08-15 01:25:05 0
MK01
 
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1+ mentions
RRID:Addgene_195090 Replaced lacI gene with chloramphenicol acetyltransferase (cat) selection cassette in the parent strain BW27783. n/a Chloramphenicol PMID:24382032 MK01 strain was derived by knocking out the endogenous lacI gene with chloramphenicol acetyltransferase (cat) selection cassette in the parent strain BW27783. The parent strain encodes araC gene but carries a deletion for the arabinose metabolizing genes, ∆(araH-araF). The all-or-none response of araBAD promoter (PBAD) response is overcome by inserting a copy of the low-affinity high-capacity transporter, araE, under the control of an arabinose-independent promoter. Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:Chloramphenicol 2026-08-15 01:25:06 2
XA103+pRCPam
 
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RRID:Addgene_195857 Amber Chromogenic Protein on pRCPam plasmid Acropora millepora Chloramphenicol PMID:38107993 Primer sequences for confirmation of suppressor mutation: Sense PCR primer (5'-->3') - TTGAGGTAATGCTTGAGATGG Antisense PCR primer (5'-->3') - TCCTGCCAGCGTTTATTG Sequencing primer (5'-->3') - CACAGCGCGTCTTTGTTTAC Vector Backbone:n/a; Vector Types:This is a strain carrying pRCPam; Bacterial Resistance:Chloramphenicol Gln62X in amber chromogenic protein 2026-08-15 01:25:07 0
XA101+pRCPam
 
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RRID:Addgene_195856 Amber Chromogenic Protein on pRCPam plasmid Acropora millepora Chloramphenicol PMID:38107993 Primer sequences for confirmation of suppressor mutation: Sense PCR primer (5'-->3') - CTGTCAGATCCATAGCGAATC Antisense PCR primer (5'-->3') - CTCTGACCTGACTGAACAATAC Sequencing primer (5'-->3') - GCTATCCGGCAGCTTCTTAAT Vector Backbone:n/a; Vector Types:This is a strain carrying pRCPam; Bacterial Resistance:Chloramphenicol Gln62X in amber chromogenic protein 2026-08-15 01:25:10 0
CSH130+pRCPam
 
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RRID:Addgene_195859 Amber Chromogenic Protein on pRCPam plasmid Acropora millepora Chloramphenicol PMID:38107993 Vector Backbone:n/a; Vector Types:This is a strain carrying pRCPam; Bacterial Resistance:Chloramphenicol Gln62X in amber chromogenic protein 2026-08-15 01:25:10 0
XA106+pRCPam
 
Resource Report
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RRID:Addgene_195858 Amber Chromogenic Protein on pRCPam plasmid Acropora millepora Chloramphenicol PMID:38107993 Primer sequences for confirmation of suppressor mutation: Sense PCR primer (5'-->3') - TCCTTCTCTGGTGTTGTTTG Antisense PCR primer (5'-->3') - TTGGCTGCTCTGACTTTG Sequencing primer (5'-->3') - CGGTTGACCTTGAGAGAGTTAAT Vector Backbone:n/a; Vector Types:This is a strain carrying pRCPam; Bacterial Resistance:Chloramphenicol Gln62X in amber chromogenic protein 2026-08-15 01:25:07 0
CSH130+pRCP
 
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RRID:Addgene_195860 Chromogenic Protein on pRCP plasmid Acropora millepora Chloramphenicol PMID:38107993 Vector Backbone:n/a; Vector Types:This is a strain carrying pRCP; Bacterial Resistance:Chloramphenicol Gln62X in amber chromogenic protein 2026-08-15 01:25:07 0
pRCP
 
Resource Report
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1+ mentions
RRID:Addgene_195862 Chromogenic Protein Acropora millepora Chloramphenicol PMID:38107993 Backbone Marker:Reference for backbone - PMID: 10723548; Vector Backbone:pDHC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:25:10 1
p15A-OptoCre-knt-P*-R
 
Resource Report
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RRID:Addgene_189850 P*-loxP-TT-loxP-R-knt Chloramphenicol PMID:36823420 Please visit https://www.biorxiv.org/content/10.1101/2022.06.10.495621v1 for bioRxiv preprint. Backbone Size:2067; Vector Backbone:pBbA-c; Vector Types:Bacterial Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:25:09 0
p15A-OptoCre-knt-P-R*
 
Resource Report
Resource Website
RRID:Addgene_189852 P-loxP-TT-loxP-R*-knt Chloramphenicol PMID:36823420 Please visit https://www.biorxiv.org/content/10.1101/2022.06.10.495621v1 for bioRxiv preprint. Backbone Size:2067; Vector Backbone:pBbA-c; Vector Types:Bacterial Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:25:09 0
pAS164
 
Resource Report
Resource Website
RRID:Addgene_194487 92-1 Op (Part 2) Synthetic Chloramphenicol PMID:36367334 Backbone Size:1658; Vector Backbone:pYKT001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:25:09 0
p15A-OptoCre-knt-P-R
 
Resource Report
Resource Website
RRID:Addgene_189849 P-loxP-TT-loxP-R-knt Chloramphenicol PMID:36823420 Please visit https://www.biorxiv.org/content/10.1101/2022.06.10.495621v1 for bioRxiv preprint. Backbone Size:2261; Vector Backbone:pBbA-c; Vector Types:Bacterial Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:25:09 0

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