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Genetic Insert: pgLAP5
Vector Backbone Description: Backbone Size:8365; Vector Backbone:pEF5/FRT-V5; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_19706 Copy
Genetic Insert: pgLAP4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7638; Vector Backbone:pEF5/FRT-V5; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_19705 Copy
Species: Streptomyces
Genetic Insert: Puro(R)
Vector Backbone Description: Vector Backbone:pGLTR-X-GFP; Vector Types:Mammalian Expression, Lentiviral, RNAi, GATEWAY destionation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24841113
Comments: There are some minor discrepancies between the depositor's assembled sequence and Addgene's quality control sequence. These differences should not affect the function of the plasmid.
Proper citation: RRID:Addgene_58246 Copy
Species: GATEWAY DEST cassette
Genetic Insert: attR1-Cm(R)-CcdB-attR2
Vector Backbone Description: Backbone Marker:Adrian Trasher, UCL, London, UK; Vector Backbone:pHR-SIN-CSGW; Vector Types:Mammalian Expression, Lentiviral, RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24841113
Comments: There are some minor discrepancies between the depositor's assembled sequence and Addgene's quality control sequence. These differences should not affect the function of the plasmid.
Proper citation: RRID:Addgene_58244 Copy
Vector Backbone Description: Backbone Size:6645; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58543 Copy
Vector Backbone Description: Backbone Size:7030; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58546 Copy
Vector Backbone Description: Backbone Size:7715; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58554 Copy
Vector Backbone Description: Backbone Size:7470; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58553 Copy
Vector Backbone Description: Backbone Size:7928; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58555 Copy
Vector Backbone Description: Backbone Size:8538; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58558 Copy
Vector Backbone Description: Backbone Size:8757; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58559 Copy
Vector Backbone Description: Backbone Size:8317; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58551 Copy
Vector Backbone Description: Backbone Marker:PMID 9733856 ; Vector Backbone:HIV-CS-CG; Vector Types:Mammalian Expression, Lentiviral, DamID; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17038565
Comments: The V5 epitope tag serves as a linker between Dam and your favorite protein.
The lentiviral vectors can be used in two ways. When transfected as a conventional plasmid, the strong viral promoter drives high expression of the Dam(fusion) proteins. This should not be used for DamID experiments, but it is useful for checking of the proteins by Western blotting or immunofluorescence microscopy. When used as lentivirus, the strong promoter is deleted upon integration of the virus, and expression of the Dam(fusion) is now driven by the weak pIND-derived heatshock promoter.
Proper citation: RRID:Addgene_59209 Copy
Vector Backbone Description: Backbone Size:7105; Vector Backbone:pDEST-0GWA; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23471584
Proper citation: RRID:Addgene_60555 Copy
Vector Backbone Description: Backbone Marker:Geneart; Vector Backbone:pAMP (Genart); Vector Types:cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24551083
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites
Proper citation: RRID:Addgene_54372 Copy
Vector Backbone Description: Backbone Marker:Geneart; Vector Backbone:pAMP (Genart); Vector Types:cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24551083
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites
Proper citation: RRID:Addgene_54371 Copy
Vector Backbone Description: Backbone Marker:Geneart; Vector Backbone:pAMP (Genart); Vector Types:cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24551083
Comments: binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites
Proper citation: RRID:Addgene_54370 Copy
Vector Backbone Description: Backbone Marker:Imre Berger; Backbone Size:7720; Vector Backbone:pFBDM; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26043233
Comments: Constructed by Christopher Weinert, Grütter lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49051 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5802; Vector Backbone:pGL4; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25177895
Proper citation: RRID:Addgene_49351 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5862; Vector Backbone:pGL4; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25177895
Comments: Please note that the plasmid sequence contains an IS1 element, and the total size of the plasmid appears to be 7.5-8kb. This difference does not affect the function of the plasmid as described in the publication.
Proper citation: RRID:Addgene_49350 Copy
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