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Genetic Insert: ffDronpa-F
Vector Backbone Description: Backbone Size:2860; Vector Backbone:pRSETb; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28561832
Proper citation: RRID:Addgene_101193 Copy
Species: Synthetic
Genetic Insert: LOV2 and STIM1 cytosolic fragment
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26646180
Proper citation: RRID:Addgene_101245 Copy
Species: Mus musculus
Genetic Insert: Rock2
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26368311
Proper citation: RRID:Addgene_101296 Copy
Species: Mus musculus
Genetic Insert: alpha-Catenin
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28329679
Comments: The TS module with mTFP1-F40-venusA206K was adapted from Vinculin TS (a kind gift from Martin Schwartz [Grashoff et al., 2010]). The TSmod was cloned in to pEGFP-N1 vector at Xho1 and Not1 restriction sites. For aE-Cat-TS, the mouse N-terminal (1_697 aa) of alpha-catenin was inserted before mTFP1 by in- fusion cloning (Takara Bioscience, Clontech) at the Xho1 restriction site
Proper citation: RRID:Addgene_101298 Copy
Species: Mus musculus
Genetic Insert: alpha-Catenin
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28329679
Comments: The C-terminal fragment (698-906 aa) of alpha-catenin was cloned after VenusA206K at the Not1 restriction site
Proper citation: RRID:Addgene_101299 Copy
Species: Other
Genetic Insert: NL4-3 envFS
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101333 Copy
Species: Other
Genetic Insert: NL4-3 envFS
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101334 Copy
Species: Other
Genetic Insert: NL4-3
Vector Backbone Description: Vector Backbone:pNL4-4; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Comments: The last methionine in Gag (M423) was left as it was outside the ApaI site used to clone in the mutations. However, C-terminus of Gag is functionally inert, and Pol slips 27 nucleotides away and is unlikely to produce a stable protein product.
Proper citation: RRID:Addgene_101342 Copy
Species: Synthetic
Genetic Insert: ASAP2s
Vector Backbone Description: Backbone Size:4562; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28749338
Comments: The next-generation JEDI-2P sensors are available at: https://www.addgene.org/browse/article/28223314/
Proper citation: RRID:Addgene_101276 Copy
Species: Mesoplasma florum
Genetic Insert: oriC2 of M. florum strain L1 (dnaA/dnaN intergenic region)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28115382
Proper citation: RRID:Addgene_101310 Copy
Species: Mus musculus
Genetic Insert: E-cadherin
Vector Backbone Description: Backbone Marker:J. Bear (UNC Chapel Hill, USA); Vector Backbone:LentiLox pLL5.0(backbone pLL3.7); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24413434
Proper citation: RRID:Addgene_101278 Copy
Species: Mesoplasma florum
Genetic Insert: oriC3 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28115382
Proper citation: RRID:Addgene_101311 Copy
Species: Homo sapiens
Genetic Insert: Utrophin
Vector Backbone Description: Vector Backbone:pCS2.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24413434
Proper citation: RRID:Addgene_101279 Copy
Species: Other
Genetic Insert: NL4-3 vpr
Vector Backbone Description: Vector Backbone:pALPS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101329 Copy
Species: Other
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pALPS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101323 Copy
Species: KSHV (viral)
Genetic Insert: LANApi TATA box mutation in pBluescript-LANApi,K14 (pDD2000).
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:7150; Vector Backbone:pBluescript II (KS+); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22740392
Comments: Addgene's sequencing found Y108H and G360D mutations in CBG68luc. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_101372 Copy
Species: KSHV (viral)
Genetic Insert: RBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000).
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:7150; Vector Backbone:pBluescript II (KS+); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22740392
Comments: Addgene's sequencing found V466A and R510C mutations in CBG68luc and a V298A mutation in CBRluc. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_101373 Copy
Species: KSHV (viral)
Genetic Insert: Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), 611-843 REV
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:7150; Vector Backbone:pBluescript II (KS+); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22740392
Proper citation: RRID:Addgene_101377 Copy
Species: Homo sapiens
Genetic Insert: BSX
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33257861
Comments: Harvard generated by gene synthesis
Proper citation: RRID:Addgene_101410 Copy
Species: KSHV (viral)
Genetic Insert: Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), RRE
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:7150; Vector Backbone:pBluescript II (KS+); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22740392
Proper citation: RRID:Addgene_101378 Copy
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